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WDR9 (BRWD1) (NM_033656) Human Tagged ORF Clone
CAT#: RG213637
- TrueORF®
BRWD1 (GFP-tagged) - Human bromodomain and WD repeat domain containing 1 (BRWD1), transcript variant 2
"NM_033656" in other vectors (2)
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Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Symbol | BRWD1 |
| Synonyms | C21orf107; DCAF19; N143; WDR9; WRD9 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG213637 representing NM_033656
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGAGCCGTCGTCCGCCCGACGCCCGGTGCCTCTCATCGAGTCGGAGCTGTACTTCCTTATCGCCC GGTACCTATCGGCGGGCCCGTGTCGGAGAGCGGCCCAGGTGCTGGTGCAGGAGCTGGAGCAGTACCAGTT GTTGCCGAAGAGATTGGACTGGGAGGGCAACGAGCACAACAGGAGCTACGAGGAGTTGGTCTTGTCCAAT AAGCATGTGGCTCCTGATCATCTTTTGCAAATCTGCCAGCGCATCGGTCCTATGTTGGATAAAGAAATTC CACCCAGTATTTCAAGAGTCACTTCTTTACTTGGTGCAGGAAGGCAGTCTTTGCTACGTACAGCAAAAGA CTGCAGGCACACAGTTTGGAAGGGCTCTGCCTTTGCTGCTCTTCATAGAGGAAGACCTCCTGAAATGCCA GTGAATTATGGTTCCCCACCAAATCTTGTGGAGATACATCGAGGAAAACAACTCACAGGGTGTTCCACTT TTAGTACAGCATTTCCAGGAACTATGTATCAGCATATAAAAATGCACAGAAGGATTCTCGGACATCTATC TGCTGTTTACTGTGTAGCATTTGATAGGACAGGACATAGAATCTTTACAGGTTCAGATGACTGTTTGGTA AAGATTTGGTCAACACATAATGGCCGCTTGTTATCTACATTAAGAGGTCATTCTGCAGAAATTTCAGATA TGGCAGTAAACTATGAGAATACAATGATTGCTGCGGGGAGCTGTGATAAAATTATTAGAGTGTGGTGCTT GAGAACTTGTGCCCCAGTTGCTGTGCTCCAAGGACACACAGGATCAATTACATCTTTACAGTTTAGCCCG ATGGCCAAAGGCTCTCAAAGATACATGGTTTCCACTGGTGCTGATGGGACAGTTTGCTTTTGGCAATGGG ATTTAGAATCCTTAAAATTTAGCCCACGTCCCCTGAAGTTCACTGAAAAGCCTAGGCCAGGCGTTCAAAT GCTTTGTTCTTCTTTTAGTGTTGGTGGTATGTTTTTAGCCACAGGTAGTACTGATCATGTAATCAGAATG TATTTTTTGGGTTTTGAAGCACCCGAAAAAATCGCAGAACTTGAAAGCCACACTGATAAAGTAGATAGTA TCCAATTTTGTAACAATGGTGATCGGTTCCTAAGTGGTAGCAGAGATGGAACAGCACGGATTTGGAGATT TGAGCAGTTAGAATGGAGGAGCATTTTATTGGATATGGCTACCAGAATCTCAGGGGACTTATCTTCCGAA GAGGAAAGGTTTATGAAACCTAAAGTAACAATGATAGCTTGGAATCAAAATGATAGCATTGTTGTCACAG CTGTGAATGATCATGTCCTCAAAGTGTGGAATTCTTACACTGGACAACTGCTTCATAACTTAATGGGACA TGCTGATGAAGTATTTGTTCTGGAGACACATCCCTTTGATTCCAGAATTATGTTATCTGCAGGACATGAT GGCAGCATATTTATATGGGATATTACAAAAGGTACCAAGATGAAACATTATTTTAATATGATTGAAGGAC AAGGACATGGAGCTGTGTTTGACTGTAAGTTTTCACAGGATGGACAGCATTTTGCCTGTACAGATTCTCA TGGGCACCTTCTGATATTTGGTTTTGGATGCAGCAAACCATATGAAAAGATTCCTGATCAGATGTTCTTC CATACTGACTATCGACCACTTATTAGAGATTCTAATAATTATGTCTTAGATGAGCAAACTCAGCAGGCTC CTCATCTTATGCCTCCACCATTCTTGGTAGATGTAGATGGAAATCCTCATCCAACCAAGTATCAGAGATT AGTACCAGGCCGAGAAAATTCTGCAGATGAACATTTGATTCCACAGCTGGGCTATGTGGCAACAAGTGAT GGAGAGGTGATTGAACAAATTATAAGCCTGCAAACCAATGATAATGATGAACGCAGCCCAGAATCGAGTA TTCTTGATGGAATGATAAGACAGTTGCAGCAGCAGCAAGATCAGAGAATGGGAGCAGATCAGGATACTAT TCCAAGAGGACTTTCAAATGGTGAAGAAACACCCCGGAGAGGTTTTAGAAGGCTGAGCTTAGACATTCAG TCCCCTCCAAATATTGGTCTGCGTCGTAGTGGACAAGTTGAAGGTGTTCGTCAGATGCATCAAAACGCTC CACGCAGTCAGATTGCTACAGAACGTGACCTGCAGGCTTGGAAACGAAGAGTGGTTGTACCAGAGGTACC ACTAGGCATATTTAGGAAGCTGGAAGACTTCCGATTAGAGAAAGGTGAAGAGGAAAGAAATCTTTATATA ATAGGAAGAAAAAGAAAGACTCTTCAGCTCTCACATAAGTCGGATTCAGTGGTTTTGGTATCACAGTCTA GACAAAGGACATGTAGGCGTAAATATCCAAATTATGGTAGAAGAAATCGTAGCTGGCGTGAGTTATCTTC TGGAAATGAGTCTTCAAGCTCTGTAAGACATGAGACTTCCTGTGATCAGAGTGAAGGTTCTGGTTCTTCA GAAGAGGATGAATGGAGAAGTGACAGAAAAAGTGAGAGTTACAGCGAAAGTTCAAGTGACTCTTCATCTA GATATTCCGATTGGACAGCTGATGCGGGCATCAATTTGCAGCCTCCTTTAAGAACATCATGTCGTCGACG AATTACTCGATTTTGTAGTAGTTCAGAAGATGAAATATCTACTGAGAATTTATCTCCTCCAAAAAGAAGA CGAAAGAGAAAGAAAGAAAATAAGCCTAAGAAGGAGAATTTGCGGAGGATGACTCCAGCAGAGCTTGCAA ATATGGAGCATTTATATGAATTTCACCCTCCAGTTTGGATTACTGACACCACACTTAGAAAATCTCCTTT TGTTCCTCAAATGGGTGATGAGGTAATATATTTTCGACAGGGTCATGAAGCTTATATTGAGGCTGTAAGA AGAAATAATATTTATGAACTGAACCCTAATAAGGAGCCATGGAGAAAAATGGATCTTAGGGATCAAGAAT TGGTTAAAATAGTTGGAATACGATATGAAGTTGGGCCCCCTACACTCTGTTGCCTAAAACTAGCATTTAT AGATCCAGCAACTGGAAAACTTATGGACAAATCTTTCTCTATTAGATATCATGATATGCCAGATGTTATT GACTTTCTTGTATTGCGTCAATTTTATGATGAAGCAAGACAGAGGAATTGGCAGTCTTGTGACAGATTCC GCTCTATTATTGATGATGCTTGGTGGTTTGGAACAGTGTTAAGTCAAGAGCCATATCAACCACAGTATCC TGATAGTCATTTCCAGTGTTATATTGTTAGGTGGGATAATACTGAAATTGAAAAACTTAGCCCATGGGAC ATGGAACCAATTCCTGATAATGTTGATCCACCTGAAGAATTAGGAGCTAGTATTTCTGTCACAACAGATG AGCTAGAGAAATTGCTCTATAAACCACAAGCTGGTGAATGGGGTCAGAAATCAAGAGATGAAGAATGTGA TAGAATTATCAGTGGTATAGATCAACTTTTGAATCTTGATATAGCAGCAGCTTTTGCAGGCCCTGTTGAT TTGTGTACATACCCGAAGTACTGTACTGTAGTAGCTTATCCAACCGATCTTTACACAATTCGAATGAGAC TTGTTAATCGATTTTACAGGAGGCTGTCTGCGTTAGTTTGGGAAGTCAGATATATAGAACATAATGCCAG AACATTTAACGAACCTGAGAGTGTAATTGCAAGATCAGCTAAAAAGATAACTGACCAACTTTTAAAATTT ATCAAGAATCAACACTGTACAAATATCTCAGAACTTTCTAACACATCTGAAAATGATGAGCAAAATGCTG AGGATTTGGATGATAGTGATCTTCCTAAAACATCTTCTGGAAGGAGGAGAGTCCATGATGGGAAAAAAAG CATCAGAGCTACGAACTATGTTGAAAGCAACTGGAAGAAACAGTGTAAGGAACTAGTGAACTTAATTTTT CAGTGTGAAGATTCTGAACCATTTAGACAACCTGTTGATTTGGTTGAATATCCAGACTACAGAGATATTA TAGATACCCCAATGGATTTTGGAACAGTAAGGGAAACTCTAGATGCGGGAAATTATGACAGCCCTTTGGA GTTTTGCAAAGACATCCGGCTGATATTTAGCAATGCAAAAGCGTATACACCAAACAAAAGATCAAAGATT TATAGTATGACCTTGAGATTATCTGCCTTATTTGAAGAAAAAATGAAGAAAATCTCTTCTGATTTTAAAA TTGGTCAAAAATTCAATGAAAAACTTCGAAGAAGCCAGAGGTTCAAGCAACGGCAAAATTGTAAAGGTGA CAGTCAGCCTAACAAAAGTATCAGAAACCTCAAGCCGAAGAGGTTAAAATCTCAGACAAAAATAATTCCT GAGTTGGTAGGTTCTCCTACCCAGTCTACCTCAAGTAGGACAGCTTATCTTGGAACCCACAAGACAAGTG CTGGTATCTCTTCAGGTGTTACTTCTGGTGACTCTTCAGATTCAGCAGAATCATCAGAAAGGAGGAAAAG AAATAGACCTATAACAAATGGTTCTACATTATCTGAAAGTGAAGTGGAAGATTCTTTAGCTACCTCTTTG TCATCGTCAGCTTCCAGTAGTTCTGAGGAAAGCAAAGAGAGTTCCAGAGCTCGTGAATCCTCCTCACGCA GTGGGCTATCCAGAAGCAGCAATCTCAGGGTAACCAGAACTAGAGCTGCTCAAAGAAAAACTGGTCCCGT TTCATTAGCAAATGGATGTGGCAGAAAAGCCACTCGAAAGAGAGTCTATTTAAGTGATTCTGATAACAAT TCATTGGAGACTGGTGAAATTCTAAAAGCCAGAGCTGGAAATAACCGAAAAGTCTTAAGGAAGTGTGCTG CTGTGGCTGCCAATAAAATAAAGCTAATGAGTGATGTAGAAGAGAATTCTAGCTCTGAAAGTGTCTGTTC TGGTCGGAAGCTGCCTCACCGCAATGCTTCTGCTGTAGCTAGAAAAAAGTTATTACATAATTCTGAAGAT GAACAGAGCTTAAAGTCAGAAATTGAAGAAGAGGAGCTAAAAGATGAAAATCAACTATTACCAGTGTCCA GTTCTCACACTGCCCAGAGCAATGTTGATGAATCTGAAAACAGAGACTCAGAGTCAGAAAGTGATTTGCG GGTAGCCCGGAAAAATTGGCATGCTAATGGTTACAAGTCCCATACTCCAGCACCTTCAAAGACAAAATTT CTTAAAATAGAGTCTTCTGAGGAAGACTCTAAAAGTCATGATTCAGATCATGCATGTAACAGAACTGCTG GCCCATCAACGTCTGTGCAGAAACTTAAGGCAGAGAGCATCTCAGAGGAAGCAGATTCTGAACCAGGAAG ATCTGGTGGTAGGAAATACAATACATTTCACAAGAATGCGAGTTTCTTTAAAAAAACCAAGATTCTGAGT GACTCAGAAGACTCTGAATCTGAAGAGCAAGATAGAGAAGATGGGAAATGTCATAAAATGGAAATGAACC CAATTTCAGGAAATCTGAACTGTGACCCTATTGCTATGTCCCAGTGTTCCTCAGATCATGGATGTGAAAC TGATTTAGATTCAGATGATGACAAAATAGAAAAACCAAACAATTTTATGAAAGATTCTGCATCACAAGAC AATGGACTAAGCAGAAAAATTTCCAGGAAAAGGGTCTGTTCCAGTGACTCAGACAGTAGTTTACAGGTGG TTAAGAAATCATCAAAAGCCAGAACAGGTCTCCTGAGGATTACTCGAAGATGTGCAGCTACGGCTGCCAA TAAGATCAAGCTCATGAGTGATGTAGAAGATGTCAGTTTAGAAAATGTGCACACTAGAAGCAAAAATGGA AGGAAAAAACCTCTCCATCTTGCTTGTACTACAGCTAAGAAGAAATTGAGTGATTGTGAAGGAAGTGTAC ATTGTGAAGTACCAAGTGAACAGTATGCCTGTGAAGGCAAGCCACCTGATCCTGACTCCGAAGGTAGTAC AAAAGTGCTTAGTCAGGCTCTAAATGGAGACTCAGACTCTGAAGATATGTTGAATTCAGAACACAAGCAC AGGCATACCAATATTCACAAAATAGATGCACCTTCTAAAAGAAAAAGTTCCTCTGTTACATCTTCAGGAG AAGATTCAAAAAGTCATATTCCAGGGAGTGAGACTGATAGGACATTTTCTTCAGAGTCAACCTTGGCACA AAAAGCTACTGCAGAGAATAATTTTGAAGTGGAACTGAATTATGGGCTGCGCAGGTGGAATGGCAGAAGA CTCAGGACCTATGGAAAGGCTCCTTTTAGTAAGACAAAAGTGATTCATGATTCACAGGAAACAGCAGAGA AGGAAGTAAAAAGGAAGAGATCGCATCCTGAATTGGAAAATGTGAAAATCTCTGAAACAACTGGGAATTC AAAGTTTAGACCTGATACTAGTTCCAAATCATCAGATTTGGGATCTGTAACTGAATCAGATATTGACTGT ACTGATAATACAAAAACCAAAAGGAGGAAAACGAAAGGAAAAGCAAAAGTAGTTAGAAAAGGTAAAACTT TTACAGCTAACATATCTAAAACTGTGAGACGTCAAAGACAAAGCAAACGCCCTAGGTTAAGTGTGGATGA TAATGACTGGGAGGATTTGGACTATGCAAAATCTAAAAGAGTTCTTCGACGTTCAAAAATAAAAACGAGA AATCAGGGTAGAAGGACTGTGAGATACCATGATGGGGATGATGACAGAAGTTTAGAAAATGTGTTAGATT TCAATGGTTGCACCTTA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG213637 representing NM_033656
Red=Cloning site Green=Tags(s) MAEPSSARRPVPLIESELYFLIARYLSAGPCRRAAQVLVQELEQYQLLPKRLDWEGNEHNRSYEELVLSN KHVAPDHLLQICQRIGPMLDKEIPPSISRVTSLLGAGRQSLLRTAKDCRHTVWKGSAFAALHRGRPPEMP VNYGSPPNLVEIHRGKQLTGCSTFSTAFPGTMYQHIKMHRRILGHLSAVYCVAFDRTGHRIFTGSDDCLV KIWSTHNGRLLSTLRGHSAEISDMAVNYENTMIAAGSCDKIIRVWCLRTCAPVAVLQGHTGSITSLQFSP MAKGSQRYMVSTGADGTVCFWQWDLESLKFSPRPLKFTEKPRPGVQMLCSSFSVGGMFLATGSTDHVIRM YFLGFEAPEKIAELESHTDKVDSIQFCNNGDRFLSGSRDGTARIWRFEQLEWRSILLDMATRISGDLSSE EERFMKPKVTMIAWNQNDSIVVTAVNDHVLKVWNSYTGQLLHNLMGHADEVFVLETHPFDSRIMLSAGHD GSIFIWDITKGTKMKHYFNMIEGQGHGAVFDCKFSQDGQHFACTDSHGHLLIFGFGCSKPYEKIPDQMFF HTDYRPLIRDSNNYVLDEQTQQAPHLMPPPFLVDVDGNPHPTKYQRLVPGRENSADEHLIPQLGYVATSD GEVIEQIISLQTNDNDERSPESSILDGMIRQLQQQQDQRMGADQDTIPRGLSNGEETPRRGFRRLSLDIQ SPPNIGLRRSGQVEGVRQMHQNAPRSQIATERDLQAWKRRVVVPEVPLGIFRKLEDFRLEKGEEERNLYI IGRKRKTLQLSHKSDSVVLVSQSRQRTCRRKYPNYGRRNRSWRELSSGNESSSSVRHETSCDQSEGSGSS EEDEWRSDRKSESYSESSSDSSSRYSDWTADAGINLQPPLRTSCRRRITRFCSSSEDEISTENLSPPKRR RKRKKENKPKKENLRRMTPAELANMEHLYEFHPPVWITDTTLRKSPFVPQMGDEVIYFRQGHEAYIEAVR RNNIYELNPNKEPWRKMDLRDQELVKIVGIRYEVGPPTLCCLKLAFIDPATGKLMDKSFSIRYHDMPDVI DFLVLRQFYDEARQRNWQSCDRFRSIIDDAWWFGTVLSQEPYQPQYPDSHFQCYIVRWDNTEIEKLSPWD MEPIPDNVDPPEELGASISVTTDELEKLLYKPQAGEWGQKSRDEECDRIISGIDQLLNLDIAAAFAGPVD LCTYPKYCTVVAYPTDLYTIRMRLVNRFYRRLSALVWEVRYIEHNARTFNEPESVIARSAKKITDQLLKF IKNQHCTNISELSNTSENDEQNAEDLDDSDLPKTSSGRRRVHDGKKSIRATNYVESNWKKQCKELVNLIF QCEDSEPFRQPVDLVEYPDYRDIIDTPMDFGTVRETLDAGNYDSPLEFCKDIRLIFSNAKAYTPNKRSKI YSMTLRLSALFEEKMKKISSDFKIGQKFNEKLRRSQRFKQRQNCKGDSQPNKSIRNLKPKRLKSQTKIIP ELVGSPTQSTSSRTAYLGTHKTSAGISSGVTSGDSSDSAESSERRKRNRPITNGSTLSESEVEDSLATSL SSSASSSSEESKESSRARESSSRSGLSRSSNLRVTRTRAAQRKTGPVSLANGCGRKATRKRVYLSDSDNN SLETGEILKARAGNNRKVLRKCAAVAANKIKLMSDVEENSSSESVCSGRKLPHRNASAVARKKLLHNSED EQSLKSEIEEEELKDENQLLPVSSSHTAQSNVDESENRDSESESDLRVARKNWHANGYKSHTPAPSKTKF LKIESSEEDSKSHDSDHACNRTAGPSTSVQKLKAESISEEADSEPGRSGGRKYNTFHKNASFFKKTKILS DSEDSESEEQDREDGKCHKMEMNPISGNLNCDPIAMSQCSSDHGCETDLDSDDDKIEKPNNFMKDSASQD NGLSRKISRKRVCSSDSDSSLQVVKKSSKARTGLLRITRRCAATAANKIKLMSDVEDVSLENVHTRSKNG RKKPLHLACTTAKKKLSDCEGSVHCEVPSEQYACEGKPPDPDSEGSTKVLSQALNGDSDSEDMLNSEHKH RHTNIHKIDAPSKRKSSSVTSSGEDSKSHIPGSETDRTFSSESTLAQKATAENNFEVELNYGLRRWNGRR LRTYGKAPFSKTKVIHDSQETAEKEVKRKRSHPELENVKISETTGNSKFRPDTSSKSSDLGSVTESDIDC TDNTKTKRRKTKGKAKVVRKGKTFTANISKTVRRQRQSKRPRLSVDDNDWEDLDYAKSKRVLRRSKIKTR NQGRRTVRYHDGDDDRSLENVLDFNGCTL TRTRRLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_033656 |
| ORF Size | 6807 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Reference Data | |
| RefSeq | NM_033656.3, NP_387505.1 |
| RefSeq Size | 17906 |
| RefSeq ORF | 6810 |
| Locus ID | 54014 |
| Domains | BROMO, WD40 |
| Gene Summary | This gene encodes a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD) residues which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes including cell cycle progression, signal transduction, apoptosis, and gene regulation. This protein contains 2 bromodomains and multiple WD repeats. This gene is located within the Down syndrome region-2 on chromosome 21. Alternative splicing of this gene generates multiple transcript variants encoding distinct isoforms. In mouse, this gene encodes a nuclear protein that has a polyglutamine-containing region that functions as a transcriptional activation domain which may regulate chromatin remodelling and associates with a component of the SWI/SNF chromatin remodelling complex. [provided by RefSeq, Jun 2011] |
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