ROBO1 (NM_002941) Human Tagged ORF Clone

CAT#: RG213799

ROBO1 (GFP-tagged) - Human roundabout, axon guidance receptor, homolog 1 (Drosophila) (ROBO1), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ROBO1
• Synonyms: DUTT1; SAX3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG213799 representing NM_002941
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAATGGAAACATGTTCCTTTTTTGGTCATGATATCACTCCTCAGCTTATCCCCAAATCACCTGTTTC
  TGGCCCAGCTTATTCCAGACCCTGAAGATGTAGAGAGGGGGAACGACCACGGGACGCCAATCCCCACCTC
  TGATAACGATGACAATTCGCTGGGCTATACAGGCTCCCGTCTTCGTCAGGAAGATTTTCCACCTCGCATT
  GTTGAACACCCTTCAGACCTGATTGTCTCAAAAGGAGAACCTGCAACTTTGAACTGCAAAGCTGAAGGCC
  GCCCCACACCCACTATTGAATGGTACAAAGGGGGAGAGAGAGTGGAGACAGACAAAGATGACCCTCGCTC
  ACACCGAATGTTGCTGCCGAGTGGATCTTTATTTTTCTTACGTATAGTACATGGACGGAAAAGTAGACCT
  GATGAAGGAGTCTATGTCTGTGTAGCAAGGAATTACCTTGGAGAGGCTGTGAGCCACAATGCATCGCTGG
  AAGTAGCCATACTTCGGGATGACTTCAGACAAAACCCTTCGGATGTCATGGTTGCAGTAGGAGAGCCTGC
  AGTAATGGAATGCCAACCTCCACGAGGCCATCCTGAGCCCACCATTTCATGGAAGAAAGATGGCTCTCCA
  CTGGATGATAAAGATGAAAGAATAACTATACGAGGAGGAAAGCTCATGATCACTTACACCCGTAAAAGTG
  ACGCTGGCAAATATGTTTGTGTTGGTACCAATATGGTTGGGGAACGTGAGAGTGAAGTAGCCGAGCTGAC
  TGTCTTAGAGAGACCATCATTTGTGAAGAGACCCAGTAACTTGGCAGTAACTGTGGATGACAGTGCAGAA
  TTTAAATGTGAGGCCCGAGGTGACCCTGTACCTACAGTACGATGGAGGAAAGATGATGGAGAGCTGCCCA
  AATCCAGATATGAAATCCGAGATGATCATACCTTGAAAATTAGGAAGGTGACAGCTGGTGACATGGGTTC
  ATACACTTGTGTTGCAGAAAATATGGTGGGCAAAGCTGAAGCATCTGCTACTCTGACTGTTCAAGAACCT
  CCACATTTTGTTGTGAAACCCCGTGACCAGGTTGTTGCTTTGGGACGGACTGTAACTTTTCAGTGTGAAG
  CAACCGGAAATCCTCAACCAGCTATTTTCTGGAGGAGAGAAGGGAGTCAGAATCTACTTTTCTCATATCA
  ACCACCACAGTCATCCAGCCGATTTTCAGTCTCCCAGACTGGCGACCTCACAATTACTAATGTCCAGCGA
  TCTGATGTTGGTTATTACATCTGCCAGACTTTAAATGTTGCTGGAAGCATCATCACAAAGGCATATTTGG
  AAGTTACAGATGTGATTGCAGATCGGCCTCCCCCAGTTATTCGACAAGGTCCTGTGAATCAGACTGTAGC
  CGTGGATGGCACTTTCGTCCTCAGCTGTGTGGCCACAGGCAGTCCAGTGCCCACCATTCTGTGGAGAAAG
  GATGGAGTCCTCGTTTCAACCCAAGACTCTCGAATCAAACAGTTGGAGAATGGAGTACTGCAGATCCGAT
  ATGCTAAGCTGGGTGATACTGGTCGGTACACCTGCATTGCATCAACCCCCAGTGGTGAAGCAACATGGAG
  TGCTTACATTGAAGTTCAAGAATTTGGAGTTCCAGTTCAGCCTCCAAGACCTACTGACCCAAATTTAATC
  CCTAGTGCCCCATCAAAACCTGAAGTGACAGATGTCAGCAGAAATACAGTCACATTATCGTGGCAACCAA
  ATTTGAATTCAGGAGCAACTCCAACATCTTATATTATAGAAGCCTTCAGCCATGCATCTGGTAGCAGCTG
  GCAGACCGTAGCAGAGAATGTGAAAACAGAAACATCTGCCATTAAAGGACTCAAACCTAATGCAATTTAC
  CTTTTCCTTGTGAGGGCAGCTAATGCATATGGAATTAGTGATCCAAGCCAAATATCAGATCCAGTGAAAA
  CACAAGATGTCCTACCAACAAGTCAGGGGGTGGACCACAAGCAGGTCCAGAGAGAGCTGGGAAATGCTGT
  TCTGCACCTCCACAACCCCACCGTCCTTTCTTCCTCTTCCATCGAAGTGCACTGGACAGTAGATCAACAG
  TCTCAGTATATACAAGGATATAAAATTCTCTATCGGCCATCTGGAGCCAACCACGGAGAATCAGACTGGT
  TAGTTTTTGAAGTGAGGACGCCAGCCAAAAACAGTGTGGTAATCCCTGATCTCAGAAAGGGAGTCAACTA
  TGAAATTAAGGCTCGCCCTTTTTTTAATGAATTTCAAGGAGCAGATAGTGAAATCAAGTTTGCCAAAACC
  CTGGAAGAAGCACCCAGTGCCCCACCCCAAGGTGTAACTGTATCCAAGAATGATGGAAACGGAACTGCAA
  TTCTAGTTAGTTGGCAGCCACCTCCAGAAGACACTCAAAATGGAATGGTCCAAGAGTATAAGGTTTGGTG
  TCTGGGCAATGAAACTCGATACCACATCAACAAAACAGTGGATGGTTCCACCTTTTCCGTGGTCATTCCC
  TTTCTTGTTCCTGGAATCCGATACAGTGTGGAAGTGGCAGCCAGCACTGGGGCTGGGTCTGGGGTAAAGA
  GTGAGCCTCAGTTCATCCAGCTGGATGCCCATGGAAACCCTGTGTCACCTGAGGACCAAGTCAGCCTCGC
  TCAGCAGATTTCAGATGTGGTGAAGCAGCCGGCCTTCATAGCAGGTATTGGAGCAGCCTGTTGGATCATC
  CTCATGGTCTTCAGCATCTGGCTTTATCGACACCGCAAGAAGAGAAACGGACTTACTAGTACCTACGCGG
  GTATCAGAAAAGTAACTTACCAGAGAGGAGGCGAAGCTGTCAGCAGTGGAGGGAGGCCTGGACTTCTCAA
  CATCAGTGAACCTGCCGCGCAGCCATGGCTGGCAGACACGTGGCCTAATACTGGCAACAACCACAATGAC
  TGCTCCATCAGCTGCTGCACGGCAGGCAATGGAAACAGCGACAGCAACCTCACTACCTACAGTCGCCCAG
  CTGATTGTATAGCAAATTATAACAACCAACTGGATAACAAACAAACAAATCTGATGCTCCCTGAGTCAAC
  TGTTTATGGTGATGTGGACCTTAGTAACAAAATCAATGAGATGAAAACCTTCAATAGCCCAAATCTGAAG
  GATGGGCGTTTTGTCAATCCATCAGGGCAGCCTACTCCTTACGCCACCACTCAGCTCATCCAGTCAAACC
  TCAGCAACAACATGAACAATGGCAGCGGGGACTCTGGCGAGAAGCACTGGAAACCACTGGGACAGCAGAA
  ACAAGAAGTGGCACCAGTTCAGTACAACATCGTGGAGCAAAACAAGCTGAACAAAGATTATCGAGCAAAT
  GACACAGTTCCTCCAACTATCCCATACAACCAATCATACGACCAGAACACAGGAGGATCCTACAACAGCT
  CAGACCGGGGCAGTAGTACATCTGGGAGTCAGGGGCACAAGAAAGGGGCAAGAACACCCAAGGTACCAAA
  ACAGGGTGGCATGAACTGGGCAGACCTGCTTCCTCCTCCCCCAGCACATCCTCCTCCACACAGCAATAGC
  GAAGAGTACAACATTTCTGTAGATGAAAGCTATGACCAAGAAATGCCATGTCCCGTGCCACCAGCAAGGA
  TGTATTTGCAACAAGATGAATTAGAAGAGGAGGAAGATGAACGAGGCCCCACTCCCCCTGTTCGGGGAGC
  AGCTTCTTCTCCAGCTGCCGTGTCCTATAGCCATCAGTCCACTGCCACTCTGACTCCCTCCCCACAGGAA
  GAACTCCAGCCCATGTTACAGGATTGTCCAGAGGAGACTGGCCACATGCAGCACCAGCCCGACAGGAGAC
  GGCAGCCTGTGAGTCCTCCTCCACCACCACGGCCGATCTCCCCTCCACATACCTATGGCTACATTTCAGG
  ACCCCTGGTCTCAGATATGGATACGGATGCGCCAGAAGAGGAAGAAGACGAAGCCGACATGGAGGTAGCC
  AAGATGCAAACCAGAAGGCTTTTGTTACGTGGGCTTGAGCAGACACCTGCCTCCAGTGTTGGGGACCTGG
  AGAGCTCTGTCACGGGGTCCATGATCAACGGCTGGGGCTCAGCCTCAGAGGAGGACAACATTTCCAGCGG
  ACGCTCCAGTGTTAGTTCTTCGGACGGCTCCTTTTTCACTGATGCTGACTTTGCCCAGGCAGTCGCAGCA
  GCGGCAGAGTATGCTGGTCTGAAAGTAGCACGACGGCAAATGCAGGATGCTGCTGGCCGTCGACATTTTC
  ATGCGTCTCAGTGCCCTAGGCCCACAAGTCCCGTGTCTACAGACAGCAACATGAGTGCCGCCGTAATGCA
  GAAAACCAGACCAGCCAAGAAACTGAAACACCAGCCAGGACATCTGCGCAGAGAAACCTACACAGATGAT
  CTTCCACCACCTCCTGTGCCGCCACCTGCTATAAAGTCACCTACTGCCCAATCCAAGACACAGCTGGAAG
  TACGACCTGTAGTGGTGCCAAAACTCCCTTCTATGGATGCAAGAACAGACAGATCATCAGACAGAAAAGG
  AAGCAGTTACAAGGGGAGAGAAGTGTTGGATGGAAGACAGGTTGTTGACATGCGAACAAATCCAGGTGAT
  CCCAGAGAAGCACAGGAACAGCAAAATGACGGGAAAGGACGTGGAAACAAGGCAGCAAAACGAGACCTTC
  CACCAGCAAAGACTCATCTCATCCAAGAGGATATTCTACCTTATTGTAGACCTACTTTTCCAACATCAAA
  TAATCCCAGAGATCCCAGTTCCTCAAGCTCAATGTCATCAAGAGGATCAGGAAGCAGACAAAGAGAACAA
  GCAAATGTAGGTCGAAGAAATATTGCAGAAATGCAGGTACTTGGAGGATATGAAAGAGGAGAAGATAATA
  ATGAAGAATTAGAGGAAACTGAAAGC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG213799 representing NM_002941
  Red=Cloning site Green=Tags(s)
  
  MKWKHVPFLVMISLLSLSPNHLFLAQLIPDPEDVERGNDHGTPIPTSDNDDNSLGYTGSRLRQEDFPPRI
  VEHPSDLIVSKGEPATLNCKAEGRPTPTIEWYKGGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRKSRP
  DEGVYVCVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGSP
  LDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNMVGERESEVAELTVLERPSFVKRPSNLAVTVDDSAE
  FKCEARGDPVPTVRWRKDDGELPKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGKAEASATLTVQEP
  PHFVVKPRDQVVALGRTVTFQCEATGNPQPAIFWRREGSQNLLFSYQPPQSSSRFSVSQTGDLTITNVQR
  SDVGYYICQTLNVAGSIITKAYLEVTDVIADRPPPVIRQGPVNQTVAVDGTFVLSCVATGSPVPTILWRK
  DGVLVSTQDSRIKQLENGVLQIRYAKLGDTGRYTCIASTPSGEATWSAYIEVQEFGVPVQPPRPTDPNLI
  PSAPSKPEVTDVSRNTVTLSWQPNLNSGATPTSYIIEAFSHASGSSWQTVAENVKTETSAIKGLKPNAIY
  LFLVRAANAYGISDPSQISDPVKTQDVLPTSQGVDHKQVQRELGNAVLHLHNPTVLSSSSIEVHWTVDQQ
  SQYIQGYKILYRPSGANHGESDWLVFEVRTPAKNSVVIPDLRKGVNYEIKARPFFNEFQGADSEIKFAKT
  LEEAPSAPPQGVTVSKNDGNGTAILVSWQPPPEDTQNGMVQEYKVWCLGNETRYHINKTVDGSTFSVVIP
  FLVPGIRYSVEVAASTGAGSGVKSEPQFIQLDAHGNPVSPEDQVSLAQQISDVVKQPAFIAGIGAACWII
  LMVFSIWLYRHRKKRNGLTSTYAGIRKVTYQRGGEAVSSGGRPGLLNISEPAAQPWLADTWPNTGNNHND
  CSISCCTAGNGNSDSNLTTYSRPADCIANYNNQLDNKQTNLMLPESTVYGDVDLSNKINEMKTFNSPNLK
  DGRFVNPSGQPTPYATTQLIQSNLSNNMNNGSGDSGEKHWKPLGQQKQEVAPVQYNIVEQNKLNKDYRAN
  DTVPPTIPYNQSYDQNTGGSYNSSDRGSSTSGSQGHKKGARTPKVPKQGGMNWADLLPPPPAHPPPHSNS
  EEYNISVDESYDQEMPCPVPPARMYLQQDELEEEEDERGPTPPVRGAASSPAAVSYSHQSTATLTPSPQE
  ELQPMLQDCPEETGHMQHQPDRRRQPVSPPPPPRPISPPHTYGYISGPLVSDMDTDAPEEEEDEADMEVA
  KMQTRRLLLRGLEQTPASSVGDLESSVTGSMINGWGSASEEDNISSGRSSVSSSDGSFFTDADFAQAVAA
  AAEYAGLKVARRQMQDAAGRRHFHASQCPRPTSPVSTDSNMSAAVMQKTRPAKKLKHQPGHLRRETYTDD
  LPPPPVPPPAIKSPTAQSKTQLEVRPVVVPKLPSMDARTDRSSDRKGSSYKGREVLDGRQVVDMRTNPGD
  PREAQEQQNDGKGRGNKAAKRDLPPAKTHLIQEDILPYCRPTFPTSNNPRDPSSSSSMSSRGSGSRQREQ
  ANVGRRNIAEMQVLGGYERGEDNNEELEETES
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002941
• ORF Size: 4926 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002941.2, NP_002932.1
• RefSeq Size: 6629 bp
• RefSeq ORF: 4956 bp
• Locus ID: 6091
• Cytogenetics: 3p12.3
• Domains: ig, IGv, IGc2, IG, FN3
• Protein Families: Druggable Genome
• Protein Pathways: Axon guidance
• Gene Summary: 'Bilateral symmetric nervous systems have special midline structures that establish a partition between the two mirror image halves. Some axons project toward and across the midline in response to long-range chemoattractants emanating from the midline. The product of this gene is a member of the immunoglobulin gene superfamily and encodes an integral membrane protein that functions in axon guidance and neuronal precursor cell migration. This receptor is activated by SLIT-family proteins, resulting in a repulsive effect on glioma cell guidance in the developing brain. A related gene is located at an adjacent region on chromosome 3. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]'