PTPRD (NM_001040712) Human Tagged ORF Clone

CAT#: RG214167

PTPRD (GFP-tagged) - Human protein tyrosine phosphatase, receptor type, D (PTPRD), transcript variant 5

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PTPRD
• Synonyms: HPTP; HPTPD; HPTPDELTA; PTPD; RPTPDELTA
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG214167 representing NM_001040712
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGCACGTAGCCAGGCTGCTGCTGCTGCTCCTCACTTTCTTCCTCCGCACGGATGCTGAGACACCTC
  CAAGGTTTACACGAACACCCGTTGATCAGACAGGGGTCTCTGGCGGAGTTGCCTCTTTCATCTGCCAAGC
  TACGGGAGACCCAAGACCTAAAATTGTCTGGAACAAAAAAGGAAAGAAAGTCAGCAATCAGAGATTTGAG
  GTAATAGAGTTTGACGATGGGTCTGGATCAGTTCTCAGAATACAACCCTTACGGACTCCGAGGGATGAGG
  CCATTTATGAATGTGTGGCCTCAAATAATGTGGGAGAAATAAGTGTATCCACCAGACTCACAGTTTTGCG
  GGAAGATCAAATTCCCAGGGGCTTCCCTACCATTGACATGGGCCCACAGTTGAAGGTGGTTGAGCGTACT
  CGCACGGCCACCATGCTTTGTGCAGCCAGTGGTAATCCGGATCCAGAAATCACTTGGTTTAAAGATTTCT
  TACCTGTGGACACAAGCAACAACAATGGTCGTATTAAGCAGTTACGATCAGGTGGTACACCAATAAGAGG
  AGCCCTTCAGATTGAGCAGAGTGAAGAGTCTGACCAAGGAAAATATGAGTGTGTTGCCACCAACAGCGCG
  GGCACTCGCTATTCCGCTCCTGCCAATTTATATGTCAGAGAGCTGCGAGAAGTTCGCCGTGTCCCACCAA
  GATTCTCTATCCCACCCACTAATCATGAAATCATGCCAGGCGGAAGCGTTAATATCACCTGTGTGGCCGT
  GGGGTCACCAATGCCTTATGTAAAGTGGATGTTGGGGGCAGAAGATCTGACACCTGAAGATGATATGCCA
  ATAGGAAGAAATGTGCTAGAACTGAATGATGTAAGACAGTCAGCAAATTACACCTGTGTTGCTATGTCAA
  CACTGGGTGTCATTGAAGCAATAGCACAGATCACTGTCAAAGCCTTACCCAAACCTCCAGGAACTCCTGT
  AGTGACCGAGAGCACAGCTACAAGCATCACACTGACGTGGGACTCTGGGAACCCTGAGCCTGTTTCTTAT
  TACATAATTCAGCATAAACCTAAAAACTCTGAGGAACTTTACAAAGAAATTGATGGGGTGGCGACCACAC
  GCTACAGTGTCGCTGGACTAAGTCCCTACTCGGATTATGAATTCAGGGTTGTTGCTGTCAATAACATTGG
  GCGGGGGCCTCCCAGCGAACCTGTGCTAACACAAACCTCAGAGCAAGCACCATCCAGTGCCCCGAGGGAT
  GTCCAGGCACGAATGTTGAGTTCGACCACCATTTTGGTACAGTGGAAGGAACCTGAAGAGCCAAATGGAC
  AGATCCAAGGATATAGAGTTTATTATACAATGGATCCCACTCAACATGTCAACAACTGGATGAAACACAA
  TGTAGCTGACAGCCAAATCACTACTATTGGCAACTTAGTGCCCCAGAAAACATATTCTGTCAAAGTCCTG
  GCTTTTACCTCAATTGGAGATGGTCCCCTTTCAAGTGACATACAAGTCATCACTCAGACAGGAGTACCAG
  GGCAGCCACTAAACTTCAAAGCAGAACCTGAGTCTGAAACAAGTATTTTGCTCTCTTGGACACCTCCACG
  TTCAGATACCATTGCCAACTATGAACTGGTCTACAAAGATGGGGAGCATGGAGAGGAGCAACGAATTACC
  ATTGAGCCAGGGACATCATATAGGCTGCAAGGACTGAAACCAAACAGCTTATACTATTTCCGTCTGGCTG
  CACGCTCCCCTCAAGGCCTGGGTGCTTCTACTGCAGAAATATCAGCTAGAACCATGCAGTCAATGTTTGC
  AAAAAATTTTCATGTCAAAGCAGTAATGAAGACTTCCGTGTTGCTGTCTTGGGAGATTCCAGAGAATTAT
  AACTCCGCCATGCCTTTCAAAATTCTTTATGATGATGGGAAAATGGTAGAAGAAGTGGATGGCCGAGCCA
  CACAGAAGTTAATTGTCAACCTGAAGCCTGAGAAATCATATTCATTTGTGCTGACAAATCGTGGAAACAG
  TGCTGGTGGGCTGCAGCACAGGGTCACGGCAAAGACTGCACCAGATGTATTACGTACCAAGCCTGCCTTC
  ATTGGGAAGACCAACTTGGATGGCATGATTACTGTGCAACTGCCTGAAGTACCTGCAAATGAGAATATAA
  AAGGTTACTACATAATAATTGTGCCTTTGAAGAAATCTCGCGGGAAATTTATCAAGCCATGGGAGAGTCC
  AGATGAAATGGAATTAGATGAGCTGCTTAAGGAGATATCTAGGAAGCGCAGAAGCATCCGTTATGGGAGA
  GAAGTTGAATTAAAGCCATATATTGCCGCTCACTTTGATGTCCTTCCCACTGAGTTCACCCTGGGGGATG
  ACAAGCATTATGGTGGATTTACAAACAAGCAACTCCAAAGTGGTCAAGAATATGTCTTCTTTGTGTTAGC
  AGTAATGGAACATGCAGAGTCTAAGATGTATGCAACCAGCCCTTACTCCGACCCCGTGGTGTCAATGGAT
  CTGGATCCGCAGCCAATCACGGATGAAGAAGAAGGCTTGATCTGGGTTGTAGGTCCTGTCCTTGCAGTGG
  TCTTTATCATCTGCATTGTCATTGCTATTCTTCTTTATAAAAGTAAACCCGACAGGAAGAGGGCAGAGTC
  CGACTCTAGAAAAAGCAGCATACCGAACAATAAGGAGATCCCTTCACACCACCCAACAGACCCTGTAGAA
  CTGAGGCGCCTTAACTTTCAAACACCGGGTATGGCTAGCCATCCTCCAATACCCATCTTGGAACTTGCAG
  ACCACATTGAAAGATTGAAAGCAAATGACAACTTGAAGTTTTCCCAGGAATATGAGTCAATTGACCCTGG
  CCAGCAGTTCACTTGGGAACATTCAAACTTGGAAGTAAACAAACCAAAGAATAGATACGCGAATGTAATC
  GCATATGATCATTCCCGGGTTCTCCTATCAGCTATAGAAGGGATCCCAGGAAGTGACTATGTGAATGCCA
  ACTACATAGATGGGTATAGGAAGCAAAATGCCTATATTGCAACACAGGGATCTCTCCCCGAAACATTTGG
  GGACTTTTGGAGAATGATATGGGAACAACGGAGTGCCACAGTTGTCATGATGACAAAACTAGAAGAAAGA
  TCAAGGGTGAAGTGTGACCAGTATTGGCCTAGCAGAGGCACAGAAACCCACGGACTCGTTCAAGTAACGC
  TGCTTGATACTGTGGAGCTGGCCACATATTGTGTTCGAACATTTGCACTTTACAAGAATGGTTCAAGTGA
  GAAGAGAGAAGTGAGACAATTCCAGTTCACCGCCTGGCCTGATCATGGTGTTCCAGAACACCCTACACCT
  TTTCTAGCTTTCTTACGTAGAGTCAAAACCTGTAACCCTCCCGATGCTGGTCCGATGGTTGTGCACTGCA
  GTGCGGGAGTTGGCCGGACTGGTTGCTTCATCGTCATAGATGCCATGTTAGAAAGAATAAAGCATGAAAA
  AACTGTAGATATTTATGGCCATGTAACTTTAATGAGAGCCCAGAGGAACTATATGGTTCAAACAGAAGAC
  CAATACATCTTTATCCATGATGCACTGTTAGAAGCAGTGACTTGTGGAAATACCGAAGTGCCAGCTAGAA
  ACTTGTATGCCTACATTCAGAAGCTGACACAAATAGAAACGGGAGAGAATGTCACAGGAATGGAGCTCGA
  ATTTAAGCGTCTAGCCAGCTCAAAAGCTCACACCTCAAGGTTTATCAGTGCCAATCTTCCATGTAATAAA
  TTCAAAAATCGCCTTGTTAATATTATGCCATATGAATCCACAAGGGTATGCCTGCAGCCTATCCGTGGAG
  TAGAAGGATCTGATTACATCAATGCCAGTTTTATTGATGGATACAGACAACAGAAAGCCTACATCGCTAC
  CCAGGGGCCCTTGGCAGAGACCACTGAAGACTTCTGGCGGATGCTCTGGGAACACAATTCCACCATAGTT
  GTGATGCTCACCAAGCTGCGTGAAATGGGCAGAGAGAAATGTCACCAATACTGGCCAGCAGAACGGTCTG
  CAAGATACCAGTACTTTGTTGTAGATCCCATGGCTGAGTACAACATGCCACAGTATATCCTAAGGGAATT
  CAAGGTCACAGATGCCAGGGACGGCCAGTCCCGAACAGTAAGGCAGTTCCAGTTCACTGACTGGCCAGAG
  CAAGGAGTGCCAAAGTCCGGAGAAGGATTTATTGACTTCATCGGCCAAGTCCATAAAACAAAAGAACAGT
  TTGGCCAAGATGGACCCATTTCAGTCCATTGCAGCGCGGGCGTTGGAAGAACTGGAGTCTTCATAACGCT
  AAGCATTGTTTTGGAAAGAATGAGATATGAAGGAGTTGTAGATATCTTCCAGACTGTCAAAATGTTAAGA
  ACACAACGACCAGCTATGGTACAGACAGAGGATCAATATCAGTTTTCCTATCGTGCCGCACTAGAGTACC
  TGGGCAGCTTTGACCACTATGCAACG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG214167 representing NM_001040712
  Red=Cloning site Green=Tags(s)
  
  MVHVARLLLLLLTFFLRTDAETPPRFTRTPVDQTGVSGGVASFICQATGDPRPKIVWNKKGKKVSNQRFE
  VIEFDDGSGSVLRIQPLRTPRDEAIYECVASNNVGEISVSTRLTVLREDQIPRGFPTIDMGPQLKVVERT
  RTATMLCAASGNPDPEITWFKDFLPVDTSNNNGRIKQLRSGGTPIRGALQIEQSEESDQGKYECVATNSA
  GTRYSAPANLYVRELREVRRVPPRFSIPPTNHEIMPGGSVNITCVAVGSPMPYVKWMLGAEDLTPEDDMP
  IGRNVLELNDVRQSANYTCVAMSTLGVIEAIAQITVKALPKPPGTPVVTESTATSITLTWDSGNPEPVSY
  YIIQHKPKNSEELYKEIDGVATTRYSVAGLSPYSDYEFRVVAVNNIGRGPPSEPVLTQTSEQAPSSAPRD
  VQARMLSSTTILVQWKEPEEPNGQIQGYRVYYTMDPTQHVNNWMKHNVADSQITTIGNLVPQKTYSVKVL
  AFTSIGDGPLSSDIQVITQTGVPGQPLNFKAEPESETSILLSWTPPRSDTIANYELVYKDGEHGEEQRIT
  IEPGTSYRLQGLKPNSLYYFRLAARSPQGLGASTAEISARTMQSMFAKNFHVKAVMKTSVLLSWEIPENY
  NSAMPFKILYDDGKMVEEVDGRATQKLIVNLKPEKSYSFVLTNRGNSAGGLQHRVTAKTAPDVLRTKPAF
  IGKTNLDGMITVQLPEVPANENIKGYYIIIVPLKKSRGKFIKPWESPDEMELDELLKEISRKRRSIRYGR
  EVELKPYIAAHFDVLPTEFTLGDDKHYGGFTNKQLQSGQEYVFFVLAVMEHAESKMYATSPYSDPVVSMD
  LDPQPITDEEEGLIWVVGPVLAVVFIICIVIAILLYKSKPDRKRAESDSRKSSIPNNKEIPSHHPTDPVE
  LRRLNFQTPGMASHPPIPILELADHIERLKANDNLKFSQEYESIDPGQQFTWEHSNLEVNKPKNRYANVI
  AYDHSRVLLSAIEGIPGSDYVNANYIDGYRKQNAYIATQGSLPETFGDFWRMIWEQRSATVVMMTKLEER
  SRVKCDQYWPSRGTETHGLVQVTLLDTVELATYCVRTFALYKNGSSEKREVRQFQFTAWPDHGVPEHPTP
  FLAFLRRVKTCNPPDAGPMVVHCSAGVGRTGCFIVIDAMLERIKHEKTVDIYGHVTLMRAQRNYMVQTED
  QYIFIHDALLEAVTCGNTEVPARNLYAYIQKLTQIETGENVTGMELEFKRLASSKAHTSRFISANLPCNK
  FKNRLVNIMPYESTRVCLQPIRGVEGSDYINASFIDGYRQQKAYIATQGPLAETTEDFWRMLWEHNSTIV
  VMLTKLREMGREKCHQYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTVRQFQFTDWPE
  QGVPKSGEGFIDFIGQVHKTKEQFGQDGPISVHCSAGVGRTGVFITLSIVLERMRYEGVVDIFQTVKMLR
  TQRPAMVQTEDQYQFSYRAALEYLGSFDHYAT
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001040712
• ORF Size: 4506 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001040712.2, NP_001035802.1
• RefSeq Size: 8257 bp
• RefSeq ORF: 4509 bp
• Locus ID: 5789
• Cytogenetics: 9p24.1-p23
• Protein Families: Druggable Genome, Phosphatase, Transmembrane
• Gene Summary: 'The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains an extracellular region, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus represents a receptor-type PTP. The extracellular region of this protein is composed of three Ig-like and eight fibronectin type III-like domains. Studies of the similar genes in chicken and fly suggest the role of this PTP is in promoting neurite growth, and regulating neurons axon guidance. Multiple alternatively spliced transcript variants of this gene have been reported. A related pseudogene has been identified on chromosome 5. [provided by RefSeq, Jan 2010]'