KIAA0319 (NM_014809) Human Tagged ORF Clone

CAT#: RG214212

KIAA0319 (GFP-tagged) - Human KIAA0319 (KIAA0319), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: KIAA0319
• Synonyms: AAVR; DYLX2; DYX2; NMIG
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG214212 representing NM_014809
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGCCCCCCACAGGTGTGCTCTCTTCATTGCTGCTGCTGGTGACAATTGCAGGTTGTGCCCGTAAGC
  AGTGCAGCGAGGGGAGGACATATTCCAATGCAGTCATTTCACCTAACTTGGAAACCACCAGAATCATGCG
  GGTGTCTCACACCTTCCCTGTCGTAGACTGCACGGCCGCTTGCTGTGACCTGTCCAGCTGTGACCTGGCC
  TGGTGGTTCGAGGGCCGCTGCTACCTGGTGAGCTGCCCCCACAAAGAGAACTGTGAGCCCAAGAAGATGG
  GCCCCATCAGGTCTTATCTCACTTTTGTGCTCCGGCCTGTTCAGAGGCCTGCACAGCTGCTGGACTATGG
  GGACATGATGCTGAACAGGGGCTCCCCCTCGGGGATCTGGGGGGACTCACCTGAGGATATCAGAAAGGAC
  TTGCCCTTTCTAGGCAAAGATTGGGGCCTAGAGGAGATGTCTGAGTACGCAGATGACTACCGGGAGCTGG
  AGAAGGACCTCTTGCAACCCAGTGGCAAGCAGGAGCCCAGAGGGAGTGCCGAGTACACGGACTGGGGCCT
  ACTGCCGGGCAGCGAGGGGGCCTTCAACTCCTCTGTTGGAGACAGTCCTGCGGTGCCAGCGGAGACGCAG
  CAGGACCCTGAGCTCCATTACCTGAATGAGTCGGCTTCAACCCCTGCCCCAAAACTCCCTGAGAGAAGTG
  TGTTGCTTCCCTTGCCGACTACTCCATCTTCAGGAGAGGTGTTGGAGAAAGAAAAGGCTTCTCAGCTCCA
  GGAACAATCCAGCAACAGCTCTGGAAAAGAGGTTCTAATGCCTTCCCATAGTCTTCCTCCGGCAAGCCTG
  GAGCTCAGCTCAGTCACCGTGGAGAAAAGCCCAGTGCTCACAGTCACCCCGGGGAGTACAGAGCACAGCA
  TCCCAACACCTCCCACTAGCGCAGCCCCCTCTGAGTCCACCCCATCTGAGCTACCCATATCTCCTACCAC
  TGCTCCCAGGACAGTGAAAGAACTTACGGTATCGGCTGGAGATAACCTAATTATAACTTTACCCGACAAT
  GAAGTTGAACTGAAGGCCTTTGTTGCGCCAGCGCCACCTGTAGAAACAACCTACAACTATGAATGGAATT
  TAATAAGCCACCCCACAGACTACCAAGGTGAAATAAAACAAGGACACAAGCAAACTCTTAACCTCTCTCA
  ATTGTCCGTCGGACTTTATGTCTTCAAAGTCACTGTTTCTAGTGAAAACGCCTTTGGAGAAGGATTTGTC
  AATGTCACTGTTAAGCCTGCCAGAAGAGTCAACCTGCCACCTGTAGCAGTTGTTTCTCCCCAACTGCAAG
  AGCTCACTTTGCCTTTGACGTCAGCCCTCATTGATGGCAGCCAAAGTACAGATGATACTGAAATAGTGAG
  TTATCATTGGGAAGAAATAAACGGGCCCTTCATAGAAGAGAAGACTTCAGTTGACTCTCCCGTCTTACGC
  TTGTCTAACCTTGATCCTGGTAACTATAGTTTCAGGTTGACTGTTACAGACTCGGACGGAGCCACTAACT
  CTACAACTGCAGCCCTAATAGTGAACAATGCTGTGGACTACCCACCAGTTGCTAATGCAGGACCAAATCA
  CACCATAACTTTGCCCCAAAACTCCATCACTTTGAATGGAAACCAGAGCAGTGACGATCACCAGATTGTC
  CTCTATGAGTGGTCCCTGGGTCCTGGGAGTGAGGGCAAACATGTGGTCATGCAGGGAGTACAGACGCCAT
  ACCTTCATTTATCTGCAATGCAGGAAGGAGATTATACATTTCAGCTGAAGGTGACAGATTCTTCAAGGCA
  ACAGTCTACTGCTGTGGTGACTGTGATTGTCCAGCCTGAAAACAATAGACCTCCAGTGGCTGTGGCCGGC
  CCTGATAAAGAGCTGATCTTCCCAGTGGAAAGTGCTACCCTGGATGGGAGCAGCAGCAGCGATGACCACG
  GCATTGTCTTCTACCACTGGGAGCACGTCAGAGGCCCCAGTGCAGTGGAGATGGAAAATATTGACAAAGC
  AATAGCCACTGTGACTGGTCTCCAGGTGGGGACCTACCACTTCCGTTTGACAGTGAAAGACCAGCAGGGA
  CTGAGCAGCACGTCCACCCTCACTGTGGCTGTGAAGAAGGAAAATAATAGTCCTCCCAGAGCCCGGGCTG
  GTGGCAGACATGTTCTTGTGCTTCCCAATAATTCCATTACTTTGGATGGTTCAAGGTCTACTGATGACCA
  AAGAATTGTGTCCTATCTGTGGATCCGGGATGGCCAGAGTCCAGCAGCTGGAGATGTCATCGATGGCTCT
  GACCACAGTGTGGCTCTGCAGCTTACGAATCTGGTGGAGGGGGTGTACACTTTCCACTTGCGAGTCACCG
  ACAGTCAGGGGGCCTCGGACACAGACACTGCCACTGTGGAAGTGCAGCCAGACCCTAGGAAGAGTGGCCT
  GGTGGAGCTGACCCTGCAGGTTGGTGTTGGGCAGCTGACAGAGCAGCGGAAGGACACCCTTGTGAGGCAG
  CTGGCTGTGCTGCTGAACGTGCTGGACTCGGACATTAAGGTCCAGAAGATTCGGGCCCACTCGGATCTCA
  GCACCGTGATTGTGTTTTATGTACAGAGCAGGCCGCCTTTCAAGGTTCTCAAAGCTGCTGAAGTGGCCCG
  AAATCTGCACATGCGGCTCTCAAAGGAGAAGGCTGACTTCTTGCTTTTCAAGGTCTTGAGGGTTGATACA
  GCAGGTTGCCTTCTGAAGTGTTCTGGCCATGGTCACTGCGACCCCCTCACAAAGCGCTGCATTTGCTCTC
  ACTTATGGATGGAGAACCTTATACAGCGTTATATCTGGGATGGAGAGAGCAACTGTGAGTGGAGTATATT
  CTATGTGACAGTGTTGGCTTTTACTCTTATTGTGCTAACAGGAGGTTTCACTTGGCTTTGCATCTGCTGC
  TGCAAAAGACAAAAAAGGACTAAAATCAGGAAAAAAACAAAGTACACCATCCTGGATAACATGGATGAAC
  AGGAAAGAATGGAACTGAGGCCCAAATATGGTATCAAGCACCGAAGCACAGAGCACAACTCCAGCCTGAT
  GGTATCCGAGTCTGAGTTTGACAGTGACCAGGACACAATCTTCAGCCGAGAAAAGATGGAGAGAGGGAAT
  CCAAAGGTTTCCATGAATGGTTCCATCAGAAATGGAGCTTCCTTCAGTTATTGCTCAAAGGACAGA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG214212 representing NM_014809
  Red=Cloning site Green=Tags(s)
  
  MAPPTGVLSSLLLLVTIAGCARKQCSEGRTYSNAVISPNLETTRIMRVSHTFPVVDCTAACCDLSSCDLA
  WWFEGRCYLVSCPHKENCEPKKMGPIRSYLTFVLRPVQRPAQLLDYGDMMLNRGSPSGIWGDSPEDIRKD
  LPFLGKDWGLEEMSEYADDYRELEKDLLQPSGKQEPRGSAEYTDWGLLPGSEGAFNSSVGDSPAVPAETQ
  QDPELHYLNESASTPAPKLPERSVLLPLPTTPSSGEVLEKEKASQLQEQSSNSSGKEVLMPSHSLPPASL
  ELSSVTVEKSPVLTVTPGSTEHSIPTPPTSAAPSESTPSELPISPTTAPRTVKELTVSAGDNLIITLPDN
  EVELKAFVAPAPPVETTYNYEWNLISHPTDYQGEIKQGHKQTLNLSQLSVGLYVFKVTVSSENAFGEGFV
  NVTVKPARRVNLPPVAVVSPQLQELTLPLTSALIDGSQSTDDTEIVSYHWEEINGPFIEEKTSVDSPVLR
  LSNLDPGNYSFRLTVTDSDGATNSTTAALIVNNAVDYPPVANAGPNHTITLPQNSITLNGNQSSDDHQIV
  LYEWSLGPGSEGKHVVMQGVQTPYLHLSAMQEGDYTFQLKVTDSSRQQSTAVVTVIVQPENNRPPVAVAG
  PDKELIFPVESATLDGSSSSDDHGIVFYHWEHVRGPSAVEMENIDKAIATVTGLQVGTYHFRLTVKDQQG
  LSSTSTLTVAVKKENNSPPRARAGGRHVLVLPNNSITLDGSRSTDDQRIVSYLWIRDGQSPAAGDVIDGS
  DHSVALQLTNLVEGVYTFHLRVTDSQGASDTDTATVEVQPDPRKSGLVELTLQVGVGQLTEQRKDTLVRQ
  LAVLLNVLDSDIKVQKIRAHSDLSTVIVFYVQSRPPFKVLKAAEVARNLHMRLSKEKADFLLFKVLRVDT
  AGCLLKCSGHGHCDPLTKRCICSHLWMENLIQRYIWDGESNCEWSIFYVTVLAFTLIVLTGGFTWLCICC
  CKRQKRTKIRKKTKYTILDNMDEQERMELRPKYGIKHRSTEHNSSLMVSESEFDSDQDTIFSREKMERGN
  PKVSMNGSIRNGASFSYCSKDR
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014809
• ORF Size: 3216 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_014809.2, NP_055624.1
• RefSeq Size: 6801
• RefSeq ORF: 3219
• Locus ID: 9856
• Domains: PKD
• Protein Families: Transmembrane
• Gene Summary: This gene encodes a transmembrane protein that contains a large extracellular domain with multiple polycystic kidney disease (PKD) domains. The encoded protein may play a role in the development of the cerebral cortex by regulating neuronal migration and cell adhesion. Single nucleotide polymorphisms in this gene are associated with dyslexia. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2011]