PPM1E (NM_014906) Human Tagged ORF Clone

CAT#: RG214622

PPM1E (GFP-tagged) - Human protein phosphatase, Mg2+/Mn2+ dependent, 1E (PPM1E)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PPM1E
• Synonyms: caMKN; CaMKP-N; POPX1; PP2CH
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG214622 representing NM_014906
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGGCTGCATCCCTGAGGAGAAAACTTACCGGCGCTTCCTGGAGCTATTCCTGGGCGAGTTTCGCG
  GACCGTGCGGCGGCGGCGAGCCGGAGCCGGAACCCGAACCCGAACCCGAACCCGAACCCGAGTCCGAGCC
  CGAGCCCGAACCTGAACTGGTAGAAGCTGAGGCGGCCGAGGCTTCGGTAGAGGAACCCGGGGAGGAGGCG
  GCCACGGTAGCCGCGACGGAGGAGGGGGACCAGGAGCAAGACCCGGAGCCCGAGGAGGAGGCGGCGGTTG
  AGGGTGAGGAGGAGGAGGAGGGCGCGGCGACGGCGGCGGCAGCCCCGGGGCACTCGGCCGTGCCGCCGCC
  GCCGCCCCAGCTGCCGCCTTTGCCCCCGCTCCCGCGACCGCTGTCAGAGCGCATCACCCGCGAGGAGGTG
  GAGGGCGAAAGCCTGGACCTGTGCCTGCAGCAGCTCTACAAATATAATTGCCCTTCCTTTTTGGCTGCTG
  CTTTAGCCAGAGCCACATCAGATGAAGTCCTTCAGAGTGATCTTTCTGCACATTATATCCCAAAGGAAAC
  GGATGGCACAGAAGGGACTGTGGAGATTGAGACAGTGAAATTGGCCCGTTCTGTCTTCAGCAAACTACAC
  GAGATTTGCTGCAGCTGGGTGAAAGACTTCCCCCTCCGCAGGAGACCCCAGCTTTATTATGAGACATCAA
  TCCATGCCATCAAAAACATGCGCAGGAAAATGGAGGACAAACATGTCTGCATTCCTGACTTTAATATGCT
  CTTCAACCTAGAGGACCAGGAAGAACAAGCTTACTTTGCAGTGTTTGATGGCCATGGGGGAGTAGATGCT
  GCTATTTATGCCTCCATTCACCTCCACGTTAACTTAGTCCGCCAGGAGATGTTCCCCCATGATCCTGCTG
  AGGCCCTGTGCAGGGCCTTCCGGGTCACTGATGAGCGGTTTGTGCAGAAAGCAGCCAGGGAGAGCTTAAG
  ATGTGGGACCACAGGAGTGGTGACTTTCATCAGAGGCAACATGCTACATGTGGCCTGGGTGGGTGATTCC
  CAGGTTATGCTTGTGAGAAAGGGCCAAGCTGTTGAACTAATGAAGCCACACAAACCAGACAGAGAGGATG
  AAAAGCAGAGAATTGAGGCCCTTGGAGGTTGCGTAGTCTGGTTTGGTGCCTGGAGGGTGAATGGAAGTCT
  GTCGGTTTCCAGAGCTATTGGAGATGCTGAACATAAGCCATATATCTGTGGGGATGCAGATTCTGCCTCC
  ACTGTTCTGGATGGGACCGAAGACTACCTCATTCTGGCCTGTGATGGCTTCTATGACACCGTGAACCCTG
  ATGAGGCAGTGAAAGTTGTGTCCGACCACCTGAAAGAGAATAATGGAGACAGCAGCATGGTTGCCCACAA
  ATTAGTGGCATCAGCTCGTGATGCTGGGTCAAGTGATAACATCACGGTTATTGTGGTATTCCTGAGGGAC
  ATGAACAAAGCTGTAAATGTTAGTGAGGAATCAGATTGGACAGAGAACTCTTTTCAAGGAGGGCAAGAAG
  ATGGTGGGGATGATAAGGAGAATCATGGAGAGTGCAAACGCCCTTGGCCTCAGCACCAGTGCTCAGCACC
  AGCCGACCTAGGCTATGATGGGCGTGTGGATTCATTCACTGATAGAACTAGCCTGAGCCCAGGGTCCCAA
  ATCAACGTGCTGGAAGACCCAGGCTACCTAGATCTCACACAAATAGAAGCAAGCAAACCTCACAGTGCCC
  AGTTTTTGCTACCAGTTGAGATGTTTGGTCCTGGTGCACCAAAGAAAGCAAATCTTATTAATGAGTTAAT
  GATGGAGAAAAAATCAGTTCAGTCATCATTGCCTGAATGGAGTGGTGCTGGAGAGTTTCCCACTGCTTTC
  AATTTGGGTTCAACAGGGGAGCAGATATACAGAATGCAGAGCTTGTCTCCTGTCTGTTCAGGGTTGGAAA
  ATGAACAGTTCAAATCCCCGGGAAACAGAGTTTCTAGATTGTCTCATTTACGCCACCACTACTCAAAGAA
  GTGGCACAGATTCAGGTTTAATCCAAAGTTTTATTCATTTCTCTCTGCTCAAGAGCCTTCCCACAAAATA
  GGCACTAGCCTGTCCTCACTTACTGGAAGTGGGAAGAGAAATAGGATAAGAAGTTCTCTGCCATGGAGGC
  AAAATAGTTGGAAAGGGTACAGTGAAAACATGAGGAAGCTCAGAAAGACTCATGATATTCCATGCCCAGA
  TCTTCCTTGGAGCTATAAAATAGAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG214622 representing NM_014906
  Red=Cloning site Green=Tags(s)
  
  MAGCIPEEKTYRRFLELFLGEFRGPCGGGEPEPEPEPEPEPEPESEPEPEPELVEAEAAEASVEEPGEEA
  ATVAATEEGDQEQDPEPEEEAAVEGEEEEEGAATAAAAPGHSAVPPPPPQLPPLPPLPRPLSERITREEV
  EGESLDLCLQQLYKYNCPSFLAAALARATSDEVLQSDLSAHYIPKETDGTEGTVEIETVKLARSVFSKLH
  EICCSWVKDFPLRRRPQLYYETSIHAIKNMRRKMEDKHVCIPDFNMLFNLEDQEEQAYFAVFDGHGGVDA
  AIYASIHLHVNLVRQEMFPHDPAEALCRAFRVTDERFVQKAARESLRCGTTGVVTFIRGNMLHVAWVGDS
  QVMLVRKGQAVELMKPHKPDREDEKQRIEALGGCVVWFGAWRVNGSLSVSRAIGDAEHKPYICGDADSAS
  TVLDGTEDYLILACDGFYDTVNPDEAVKVVSDHLKENNGDSSMVAHKLVASARDAGSSDNITVIVVFLRD
  MNKAVNVSEESDWTENSFQGGQEDGGDDKENHGECKRPWPQHQCSAPADLGYDGRVDSFTDRTSLSPGSQ
  INVLEDPGYLDLTQIEASKPHSAQFLLPVEMFGPGAPKKANLINELMMEKKSVQSSLPEWSGAGEFPTAF
  NLGSTGEQIYRMQSLSPVCSGLENEQFKSPGNRVSRLSHLRHHYSKKWHRFRFNPKFYSFLSAQEPSHKI
  GTSLSSLTGSGKRNRIRSSLPWRQNSWKGYSENMRKLRKTHDIPCPDLPWSYKIE
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014906
• ORF Size: 2265 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_014906.3, NP_055721.3
• RefSeq Size: 6545
• RefSeq ORF: 2268
• Locus ID: 22843
• Domains: PP2C
• Protein Families: Druggable Genome, Phosphatase
• Gene Summary: This gene encodes a member of the PPM family of serine/threonine-protein phosphatases. The encoded protein is localized to the nucleus and dephosphorylates and inactivates multiple substrates including serine/threonine-protein kinase PAK 1, 5'-AMP-activated protein kinase (AMPK) and the multifunctional calcium/calmodulin-dependent protein kinases. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, May 2012]