RO60 (NM_001042369) Human Tagged ORF Clone

CAT#: RG214690

TROVE2 (GFP-tagged) - Human TROVE domain family, member 2 (TROVE2), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: RO60
• Synonyms: RORNP; SSA2; TROVE2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG214690 representing NM_001042369
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGAATCTGTAAACCAAATGCAGCCACTGAATGAGAAGCAGATAGCCAATTCTCAGGATGGATATG
  TATGGCAAGTCACTGACATGAATCGACTACACCGGTTCTTATGTTTCGGTTCTGAAGGTGGGACTTATTA
  TATCAAAGAACAGAAGTTGGGCCTTGAAAATGCTGAAGCTTTAATTAGATTGATTGAAGATGGCAGAGGA
  TGTGAAGTGATACAAGAAATAAAGTCATTTAGTCAAGAAGGCAGAACCACAAAGCAAGAGCCTATGCTCT
  TTGCACTTGCCATTTGTTCCCAGTGCTCCGACATAAGCACAAAACAAGCAGCATTTAAAGCTGTTTCTGA
  AGTTTGTCGCATTCCTACCCATCTCTTTACTTTTATCCAGTTTAAGAAAGATCTGAAGGAAAGCATGAAA
  TGTGGCATGTGGGGTCGTGCCCTCCGGAAGGCTATAGCGGACTGGTACAATGAGAAAGGTGGCATGGCCC
  TTGCTCTGGCAGTTACAAAATATAAACAGAGAAATGGCTGGTCTCACAAAGATCTATTAAGATTGTCACA
  TCTTAAACCTTCCAGTGAAGGACTTGCAATTGTGACCAAATATATTACAAAGGGCTGGAAAGAAGTTCAT
  GAATTGTATAAAGAAAAAGCACTCTCTGTGGAGACTGAAAAATTATTAAAGTATCTGGAGGCTGTAGAGA
  AAGTGAAGCGCACAAGAGATGAGCTAGAAGTCATTCATCTAATAGAAGAACATAGATTAGTTAGAGAACA
  TCTTTTAACAAATCACTTAAAGTCTAAAGAGGTATGGAAGGCTTTGTTACAAGAAATGCCGCTTACTGCA
  TTACTAAGGAATCTAGGAAAGATGACTGCTAATTCAGTACTTGAACCAGGAAATTCAGAAGTATCTTTAG
  TATGTGAAAAACTGTGTAATGAAAAACTATTAAAAAAGGCTCGTATACATCCATTTCATATTTTGATCGC
  ATTAGAAACTTACAAGACAGGTCATGGTCTCAGAGGGAAACTGAAGTGGCGCCCTGATGAAGAAATTTTG
  AAAGCATTGGATGCTGCTTTTTATAAAACATTTAAGACAGTTGAACCAACTGGAAAACGTTTCTTACTAG
  CTGTTGATGTCAGTGCTTCTATGAACCAAAGAGTTTTGGGTAGTATACTCAACGCTAGTACAGTTGCTGC
  AGCAATGTGCATGGTTGTCACACGAACAGAAAAAGATTCTTATGTAGTTGCTTTTTCCGATGAAATGGTA
  CCATGTCCAGTGACTACAGATATGACCTTACAACAGGTTTTAATGGCTATGAGTCAGATCCCAGCAGGTG
  GAACTGATTGCTCTCTTCCAATGATCTGGGCTCAGAAGACAAACACACCTGCTGATGTCTTCATTGTATT
  CACTGATAATGAGACCTTTGCTGGAGGTGTCCATCCTGCTATTGCTCTGAGGGAGTATCGAAAGAAAATG
  GATATTCCAGCTAAATTGATTGTTTGTGGAATGACATCAAATGGTTTCACCATTGCAGACCCAGATGATA
  GAGCCTTGCAAAATACCCTACTAAATAAATCATTT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG214690 representing NM_001042369
  Red=Cloning site Green=Tags(s)
  
  MEESVNQMQPLNEKQIANSQDGYVWQVTDMNRLHRFLCFGSEGGTYYIKEQKLGLENAEALIRLIEDGRG
  CEVIQEIKSFSQEGRTTKQEPMLFALAICSQCSDISTKQAAFKAVSEVCRIPTHLFTFIQFKKDLKESMK
  CGMWGRALRKAIADWYNEKGGMALALAVTKYKQRNGWSHKDLLRLSHLKPSSEGLAIVTKYITKGWKEVH
  ELYKEKALSVETEKLLKYLEAVEKVKRTRDELEVIHLIEEHRLVREHLLTNHLKSKEVWKALLQEMPLTA
  LLRNLGKMTANSVLEPGNSEVSLVCEKLCNEKLLKKARIHPFHILIALETYKTGHGLRGKLKWRPDEEIL
  KALDAAFYKTFKTVEPTGKRFLLAVDVSASMNQRVLGSILNASTVAAAMCMVVTRTEKDSYVVAFSDEMV
  PCPVTTDMTLQQVLMAMSQIPAGGTDCSLPMIWAQKTNTPADVFIVFTDNETFAGGVHPAIALREYRKKM
  DIPAKLIVCGMTSNGFTIADPDDRALQNTLLNKSF
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001042369
• ORF Size: 1575 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001042369.1, NP_001035828.1
• RefSeq Size: 2050 bp
• RefSeq ORF: 1578 bp
• Locus ID: 6738
• Cytogenetics: 1q31.2
• Protein Families: Transcription Factors
• Protein Pathways: Systemic lupus erythematosus
• MW: 59.3 kDa
• Gene Summary: ''