Beta TRCP (BTRC) (NM_003939) Human Tagged ORF Clone

CAT#: RG214799

BTRC (GFP-tagged) - Human beta-transducin repeat containing (BTRC), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: BTRC
• Synonyms: BETA-TRCP; betaTrCP; bTrCP; bTrCP1; FBW1A; FBXW1; FBXW1A; FWD1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG214799 representing NM_003939
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACCCGGCCGAGGCGGTGCTGCAAGAGAAGGCACTCAAGTTTATGAATTCCTCAGAGAGAGAAGACT
  GTAATAATGGCGAACCCCCTAGGAAGATAATACCAGAGAAGAATTCACTTAGACAGACATACAACAGCTG
  TGCCAGACTCTGCTTAAACCAAGAAACAGTATGTTTAGCAAGCACTGCTATGAAGACTGAGAATTGTGTG
  GCCAAAACAAAACTTGCCAATGGCACTTCCAGTATGATTGTGCCCAAGCAACGGAAACTCTCAGCAAGCT
  ATGAAAAGGAAAAGGAACTGTGTGTCAAATACTTTGAGCAGTGGTCAGAGTCAGATCAAGTGGAATTTGT
  GGAACATCTTATATCCCAAATGTGTCATTACCAACATGGGCACATAAACTCGTATCTTAAACCTATGTTG
  CAGAGAGATTTCATAACTGCTCTGCCAGCTCGGGGATTGGATCATATTGCTGAGAACATTCTGTCATACC
  TGGATGCCAAATCACTATGTGCTGCTGAACTTGTGTGCAAGGAATGGTACCGAGTGACCTCTGATGGCAT
  GCTGTGGAAGAAGCTTATCGAGAGAATGGTCAGGACAGATTCTCTGTGGAGAGGCCTGGCAGAACGAAGA
  GGATGGGGACAGTATTTATTCAAAAACAAACCTCCTGACGGGAATGCTCCTCCCAACTCTTTTTATAGAG
  CACTTTATCCTAAAATTATACAAGACATTGAGACAATAGAATCTAATTGGAGATGTGGAAGACATAGTTT
  ACAGAGAATTCACTGCCGAAGTGAAACAAGCAAAGGAGTTTACTGTTTACAGTATGATGATCAGAAAATA
  GTAAGCGGCCTTCGAGACAACACAATCAAGATCTGGGATAAAAACACATTGGAATGCAAGCGAATTCTCA
  CAGGCCATACAGGTTCAGTCCTCTGTCTCCAGTATGATGAGAGAGTGATCATAACAGGATCATCGGATTC
  CACGGTCAGAGTGTGGGATGTAAATACAGGTGAAATGCTAAACACGTTGATTCACCATTGTGAAGCAGTT
  CTGCACTTGCGTTTCAATAATGGCATGATGGTGACCTGCTCCAAAGATCGTTCCATTGCTGTATGGGATA
  TGGCCTCCCCAACTGACATTACCCTCCGGAGGGTGCTGGTCGGACACCGAGCTGCTGTCAATGTTGTAGA
  CTTTGATGACAAGTACATTGTTTCTGCATCTGGGGATAGAACTATAAAGGTATGGAACACAAGTACTTGT
  GAATTTGTAAGGACCTTAAATGGACACAAACGAGGCATTGCCTGTTTGCAGTACAGGGACAGGCTGGTAG
  TGAGTGGCTCATCTGACAACACTATCAGATTATGGGACATAGAATGTGGTGCATGTTTACGAGTGTTAGA
  AGGCCATGAGGAATTGGTGCGTTGTATTCGATTTGATAACAAGAGGATAGTCAGTGGGGCCTATGATGGA
  AAAATTAAAGTGTGGGATCTTGTGGCTGCTTTGGACCCCCGTGCTCCTGCAGGGACACTCTGTCTACGGA
  CCCTTGTGGAGCATTCCGGAAGAGTTTTTCGACTACAGTTTGATGAATTCCAGATTGTCAGTAGTTCACA
  TGATGACACAATCCTCATCTGGGACTTCCTAAATGATCCAGCTGCCCAAGCTGAACCCCCCCGTTCCCCT
  TCTCGAACATACACCTACATCTCCAGA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG214799 representing NM_003939
  Red=Cloning site Green=Tags(s)
  
  MDPAEAVLQEKALKFMNSSEREDCNNGEPPRKIIPEKNSLRQTYNSCARLCLNQETVCLASTAMKTENCV
  AKTKLANGTSSMIVPKQRKLSASYEKEKELCVKYFEQWSESDQVEFVEHLISQMCHYQHGHINSYLKPML
  QRDFITALPARGLDHIAENILSYLDAKSLCAAELVCKEWYRVTSDGMLWKKLIERMVRTDSLWRGLAERR
  GWGQYLFKNKPPDGNAPPNSFYRALYPKIIQDIETIESNWRCGRHSLQRIHCRSETSKGVYCLQYDDQKI
  VSGLRDNTIKIWDKNTLECKRILTGHTGSVLCLQYDERVIITGSSDSTVRVWDVNTGEMLNTLIHHCEAV
  LHLRFNNGMMVTCSKDRSIAVWDMASPTDITLRRVLVGHRAAVNVVDFDDKYIVSASGDRTIKVWNTSTC
  EFVRTLNGHKRGIACLQYRDRLVVSGSSDNTIRLWDIECGACLRVLEGHEELVRCIRFDNKRIVSGAYDG
  KIKVWDLVAALDPRAPAGTLCLRTLVEHSGRVFRLQFDEFQIVSSSHDDTILIWDFLNDPAAQAEPPRSP
  SRTYTYISR
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003939
• ORF Size: 1707 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_003939.2, NP_003930.1
• RefSeq Size: 6038
• RefSeq ORF: 1710
• Locus ID: 8945
• Domains: WD40, F-box
• Protein Families: Druggable Genome
• Protein Pathways: Hedgehog signaling pathway, Oocyte meiosis, Ubiquitin mediated proteolysis, Wnt signaling pathway
• Gene Summary: This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbws class; in addition to an F-box, this protein contains multiple WD-40 repeats. The encoded protein mediates degradation of CD4 via its interaction with HIV-1 Vpu. It has also been shown to ubiquitinate phosphorylated NFKBIA (nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha), targeting it for degradation and thus activating nuclear factor kappa-B. Alternatively spliced transcript variants have been described. A related pseudogene exists in chromosome 6. [provided by RefSeq, Mar 2012]