GPR136 (OPN5) (NM_181744) Human Tagged ORF Clone

CAT#: RG214800

OPN5 (GFP-tagged) - Human opsin 5 (OPN5), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: OPN5
• Synonyms: GPR136; GRP136; PGR12; TMEM13
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG214800 representing NM_181744
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGTTAAATCACACTGCCCTGCCTCAGGACGAGCGCCTGCCCCATTACCTTCGAGATGGGGATCCTT
  TTGCTTCCAAACTTTCTTGGGAAGCGGATTTAGTGGCTGGCTTTTACCTAACAATAATTGGGATTCTGTC
  CACATTTGGAAATGGATATGTCCTTTACATGTCTTCTAGACGAAAGAAGAAGCTGAGACCCGCTGAAATA
  ATGACTATCAATTTAGCAGTCTGTGATCTGGGGATTTCAGTTGTAGGCAAGCCGTTCACCATCATCTCTT
  GCTTTTGTCACCGCTGGGTGTTTGGCTGGATCGGCTGCCGCTGGTATGGATGGGCTGGATTTTTCTTTGG
  CTGTGGAAGCCTTATCACCATGACTGCTGTCAGCCTGGATCGATATTTGAAAATCTGCTATTTATCTTAT
  GGGGTTTGGCTGAAAAGAAAGCACGCCTACATCTGCCTGGCAGCCATCTGGGCCTATGCTTCCTTCTGGA
  CCACCATGCCCTTGGTAGGTCTGGGGGACTACGTACCTGAGCCCTTCGGAACCTCGTGCACCCTGGACTG
  GTGGCTGGCCCAGGCCTCGGTAGGGGGCCAGGTTTTCATCCTGAACATCCTCTTCTTCTGCCTCTTGCTC
  CCAACGGCTGTGATCGTGTTCTCCTACGTAAAGATCATTGCCAAGGTTAAGTCCTCTTCCAAAGAAGTAG
  CTCATTTCGACAGTCGGATCCATAGCAGCCATGTGCTGGAAATGAAACTGACAAAGGTAGCGATGTTGAT
  TTGTGCTGGATTCCTGATTGCCTGGATTCCTTATGCAGTGGTGTCTGTGTGGTCAGCTTTTGGAAGGCCA
  GACTCCATTCCCATACAGCTCTCTGTGGTGCCAACCCTACTTGCAAAATCTGCAGCGATGTACAATCCCA
  TCATTTACCAAGTTATTGATTACAAATTTGCCTGTTGCCAAACTGGTGGTTTGAAAGCAACCAAGAAGAA
  GTCTCTGGAAGGCTTCAGGCTGCACACCGTAACCACAGTCAGGAAGTCTTCTGCTGTGCTGGAAATTCAT
  GAAGAGTGGGAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG214800 representing NM_181744
  Red=Cloning site Green=Tags(s)
  
  MALNHTALPQDERLPHYLRDGDPFASKLSWEADLVAGFYLTIIGILSTFGNGYVLYMSSRRKKKLRPAEI
  MTINLAVCDLGISVVGKPFTIISCFCHRWVFGWIGCRWYGWAGFFFGCGSLITMTAVSLDRYLKICYLSY
  GVWLKRKHAYICLAAIWAYASFWTTMPLVGLGDYVPEPFGTSCTLDWWLAQASVGGQVFILNILFFCLLL
  PTAVIVFSYVKIIAKVKSSSKEVAHFDSRIHSSHVLEMKLTKVAMLICAGFLIAWIPYAVVSVWSAFGRP
  DSIPIQLSVVPTLLAKSAAMYNPIIYQVIDYKFACCQTGGLKATKKKSLEGFRLHTVTTVRKSSAVLEIH
  EEWE
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_181744
• ORF Size: 1062 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_181744.2, NP_859528.1
• RefSeq Size: 3473
• RefSeq ORF: 1065
• Locus ID: 221391
• Protein Families: Druggable Genome, Transmembrane
• Gene Summary: Opsins are members of the guanine nucleotide-binding protein (G protein)-coupled receptor superfamily. This opsin gene is expressed in the eye, brain, testes, and spinal cord. This gene belongs to the seven-exon subfamily of mammalian opsin genes that includes peropsin (RRH) and retinal G protein coupled receptor (RGR). Like these other seven-exon opsin genes, this family member may encode a protein with photoisomerase activity. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2010]