THBS3 (NM_007112) Human Tagged ORF Clone

CAT#: RG214968

THBS3 (GFP-tagged) - Human thrombospondin 3 (THBS3)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: THBS3
• Synonyms: TSP3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG214968 representing NM_007112
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGACGCAGGAACTTCGGGGGGCCCTGGCTCTTCTCCTCCTTTGCTTTTTCACATCTGCCAGTCAGG
  ATCTGCAGGTAATTGACCTGCTGACTGTGGGTGAGTCTCGGCAGATGGTAGCTGTGGCAGAGAAGATCCG
  GACAGCCTTGCTCACTGCTGGGGACATCTACCTCTTATCCACCTTCCGCCTGCCCCCCAAGCAGGGTGGT
  GTCCTCTTTGGCCTCTATTCTCGCCAAGACAACACTCGATGGCTGGAGGCCTCTGTTGTAGGCAAGATCA
  ACAAAGTACTGGTGCGATACCAGCGGGAGGATGGCAAAGTCCACGCCGTGAACCTACAGCAAGCGGGCCT
  GGCTGATGGGCGCACACACACAGTTCTCCTGCGACTCCGAGGTCCCTCCAGACCCAGCCCTGCCCTACAT
  CTCTACGTGGACTGCAAACTGGGTGACCAACATGCAGGCCTTCCAGCACTGGCCCCCATTCCTCCAGCGG
  AGGTCGATGGGCTGGAGATTAGGACTGGACAGAAGGCGTATTTGAGGATGCAGGGCTTTGTGGAATCTAT
  GAAAATTATTCTGGGTGGGTCCATGGCCCGGGTAGGAGCCCTGAGTGAGTGTCCATTCCAAGGGGACGAG
  TCCATCCACAGTGCAGTGACCAATGCACTGCACTCCATTCTAGGGGAGCAGACCAAGGCGCTGGTCACCC
  AACTCACCCTCTTCAACCAGATCCTGGTGGAGCTGCGGGATGATATACGAGACCAGGTGAAGGAAATGTC
  CCTGATCCGAAACACCATTATGGAGTGTCAGGTGTGCGGCTTCCATGAGCAGCGTTCCCACTGCAGCCCC
  AATCCCTGCTTCCGAGGTGTGGACTGCATGGAAGTGTACGAGTACCCAGGCTACCGCTGTGGGCCCTGCC
  CCCCTGGCCTGCAGGGCAACGGCACCCACTGCAGTGACATCAATGAGTGTGCTCACGCTGACCCCTGTTT
  CCCGGGCTCCAGCTGCATCAACACCATGCCCGGCTTCCACTGTGAGGCCTGTCCTCGAGGGTACAAGGGC
  ACACAGGTGTCTGGTGTGGGCATTGACTATGCCCGGGCCAGCAAACAGGTCTGCAATGACATCGATGAAT
  GCAACGATGGCAACAATGGTGGCTGTGACCCAAACTCCATCTGCACCAACACTGTGGGCTCTTTCAAGTG
  TGGTCCCTGCCGCCTGGGTTTCCTGGGCAACCAGAGCCAGGGCTGCCTCCCAGCCCGGACCTGCCACAGC
  CCAGCCCACAGCCCCTGCCACATCCATGCTCACTGTCTCTTTGAACGCAATGGTGCAGTGTCCTGCCAGT
  GTAACGTGGGCTGGGCTGGGAATGGGAACGTGTGTGGGACTGACACAGACATCGATGGCTACCCAGACCA
  AGCACTGCCCTGCATGGACAACAACAAACACTGCAAACAGGACAACTGCCTTTTGACACCCAACTCTGGG
  CAGGAAGATGCTGATAATGATGGTGTGGGGGACCAGTGTGATGATGATGCTGATGGGGATGGGATCAAGA
  ATGTTGAGGACAACTGCCGGCTGTTCCCCAACAAAGACCAGCAGAACTCAGATACAGATTCATTTGGTGA
  TGCCTGTGACAATTGCCCCAACGTTCCCAACAATGACCAGAAGGACACAGATGGCAATGGGGAAGGAGAT
  GCCTGTGACAACGACGTGGATGGGGATGGCATCCCCAATGGATTGGACAATTGCCCTAAAGTCCCCAACC
  CACTACAGACAGACAGGGATGAGGACGGGGTGGGAGATGCTTGCGACAGCTGCCCTGAAATGAGCAATCC
  TACCCAGACAGATGCAGACAGCGACCTGGTGGGGGATGTCTGTGATACTAATGAAGACAGCGATGGGGAT
  GGGCATCAGGACACCAAGGACAACTGCCCACAGCTGCCAAATAGCTCCCAGCTGGACTCTGATAACGATG
  GACTTGGAGATGAGTGTGATGGGGATGATGACAATGATGGCATCCCAGATTATGTGCCTCCTGGTCCCGA
  TAACTGCCGCCTGGTACCCAATCCCAATCAGAAGGACTCAGATGGCAATGGCGTTGGTGATGTGTGTGAG
  GATGACTTTGACAATGATGCTGTGGTCGACCCCCTGGATGTGTGTCCTGAAAGTGCAGAGGTAACGCTTA
  CGGATTTTCGGGCCTATCAGACCGTCGTCCTGGATCCTGAGGGTGATGCTCAGATTGACCCAAACTGGGT
  TGTGCTCAACCAGGGCATGGAAATCGTTCAGACCATGAACAGTGACCCTGGCTTGGCAGTTGGATACACG
  GCCTTCAATGGTGTGGACTTTGAAGGCACCTTCCATGCGAACACAGTGACTGATGATGACTACGCAGGCT
  TTCTCTTCAGTTATCAAGACAGTGGCCGCTTCTACGTAGTCATGTGGAAGCAGACCGAGCAGACCTACTG
  GCAGGCTACACCCTTCCGGGCGGTTGCCCAGCCCGGGCTGCAGCTCAAGGCAGTGACATCAGTGTCTGGC
  CCAGGTGAGCACCTCCGAAATGCCCTGTGGCATACTGGCCACACCCCTGATCAGGTACGACTGCTGTGGA
  CAGACCCACGAAATGTGGGCTGGCGGGACAAGACCTCCTATCGCTGGCAGCTTCTGCACCGGCCTCAAGT
  TGGCTACATTCGGGTGAAGCTCTATGAGGGACCCCAGCTTGTGGCGGATTCTGGGGTGATCATTGACACA
  TCCATGCGAGGGGGGCGTCTTGGTGTATTCTGCTTCTCCCAAGAAAACATAATTTGGTCCAATCTCCAGT
  ATCGATGCAATGACACAGTGCCTGAGGACTTTGAGCCATTCCGGAGGCAGCTGCTCCAGGGAAGGGTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG214968 representing NM_007112
  Red=Cloning site Green=Tags(s)
  
  METQELRGALALLLLCFFTSASQDLQVIDLLTVGESRQMVAVAEKIRTALLTAGDIYLLSTFRLPPKQGG
  VLFGLYSRQDNTRWLEASVVGKINKVLVRYQREDGKVHAVNLQQAGLADGRTHTVLLRLRGPSRPSPALH
  LYVDCKLGDQHAGLPALAPIPPAEVDGLEIRTGQKAYLRMQGFVESMKIILGGSMARVGALSECPFQGDE
  SIHSAVTNALHSILGEQTKALVTQLTLFNQILVELRDDIRDQVKEMSLIRNTIMECQVCGFHEQRSHCSP
  NPCFRGVDCMEVYEYPGYRCGPCPPGLQGNGTHCSDINECAHADPCFPGSSCINTMPGFHCEACPRGYKG
  TQVSGVGIDYARASKQVCNDIDECNDGNNGGCDPNSICTNTVGSFKCGPCRLGFLGNQSQGCLPARTCHS
  PAHSPCHIHAHCLFERNGAVSCQCNVGWAGNGNVCGTDTDIDGYPDQALPCMDNNKHCKQDNCLLTPNSG
  QEDADNDGVGDQCDDDADGDGIKNVEDNCRLFPNKDQQNSDTDSFGDACDNCPNVPNNDQKDTDGNGEGD
  ACDNDVDGDGIPNGLDNCPKVPNPLQTDRDEDGVGDACDSCPEMSNPTQTDADSDLVGDVCDTNEDSDGD
  GHQDTKDNCPQLPNSSQLDSDNDGLGDECDGDDDNDGIPDYVPPGPDNCRLVPNPNQKDSDGNGVGDVCE
  DDFDNDAVVDPLDVCPESAEVTLTDFRAYQTVVLDPEGDAQIDPNWVVLNQGMEIVQTMNSDPGLAVGYT
  AFNGVDFEGTFHANTVTDDDYAGFLFSYQDSGRFYVVMWKQTEQTYWQATPFRAVAQPGLQLKAVTSVSG
  PGEHLRNALWHTGHTPDQVRLLWTDPRNVGWRDKTSYRWQLLHRPQVGYIRVKLYEGPQLVADSGVIIDT
  SMRGGRLGVFCFSQENIIWSNLQYRCNDTVPEDFEPFRRQLLQGRV
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007112
• ORF Size: 2868 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007112.3, NP_009043.1
• RefSeq Size: 3176 bp
• RefSeq ORF: 2871 bp
• Locus ID: 7059
• Cytogenetics: 1q22
• Protein Families: Druggable Genome
• Protein Pathways: ECM-receptor interaction, Focal adhesion, TGF-beta signaling pathway
• Gene Summary: 'The protein encoded by this gene belongs to the thrombospondin family. Thrombospondin family members are adhesive glycoproteins that mediate cell-to-cell and cell-to-matrix interactions. This protein forms a pentameric molecule linked by a single disulfide bond. This gene shares a common promoter with metaxin 1. Alternate splicing results in coding and non-coding transcript variants. [provided by RefSeq, Nov 2011]'