ETNK2 (NM_018208) Human Tagged ORF Clone

CAT#: RG214974

ETNK2 (GFP-tagged) - Human ethanolamine kinase 2 (ETNK2)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ETNK2
• Synonyms: EKI2; HMFT1716
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG214974 representing NM_018208
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGTGCCCCCTTCGGCCCCTCAGCAGCGCGCGTCCTTTCACCTGAGGAGGCACACGCCTTGCCCGC
  AGTGCTCATGGGGCATGGAGGAGAAGGCGGCGGCCAGCGCCAGCTGCCGGGAGCCGCCGGGCCCCCCGAG
  GGCCGCCGCCGTCGCGTACTTCGGCATTTCCGTGGACCCGGACGACATCCTTCCCGGGGCCCTGCGCCTC
  ATCCAGGAGCTGCGGCCGCATTGGAAACCCGAGCAAGTTCGGACCAAGCGCTTCACGGATGGCATCACCA
  ACAAGCTGGTGGCCTGCTATGTGGAGGAGGACATGCAGGACTGCGTGCTGGTCCGGGTGTATGGGGAGCG
  GACGGAGCTGCTGGTGGACCGGGAGAATGAGGTCAGAAACTTCCAGCTGCTGCGAGCACACAGCTGTGCC
  CCCAAACTCTACTGCACCTTCCAGAATGGGCTGTGCTATGAGTACATGCAGGGTGTGGCCCTGGAGCCTG
  AGCACATCCGTGAGCCCCGGCTTTTCAGGTTAATCGCCTTAGAAATGGCAAAGATTCATACTATCCACGC
  CAACGGCAGCCTGCCCAAGCCCATCCTCTGGCACAAGATGCACAATTATTTCACGCTTGTGAAGAACGAG
  ATCAACCCCAGCCTTTCTGCAGATGTCCCTAAGGTAGAGGTGTTGGAACGGGAGCTGGCCTGGCTGAAGG
  AGCATCTGTCCCAGCTGGAGTCCCCTGTGGTGTTTTGTCACAATGACCTGCTCTGCAAGAATATCATCTA
  TGACAGCATCAAAGGTCACGTGCGGTTCATTGACTATGAATATGCTGGCTACAACTACCAAGCTTTTGAC
  ATTGGCAACCATTTCAATGAGTTTGCAGGCGTGAATGAGGTGGATTACTGCCTGTACCCGGCGCGGGAGA
  CCCAGCTGCAGTGGCTGCACTACTACCTGCAGGCACAAAAGGGGATGGCCGTGACCCCCAGGGAGGTGCA
  AAGGCTCTACGTGCAAGTCAACAAGTTTGCCCTGGGTCCTAGCTGTGTGTCTTCCACAATGACTGCATCC
  CTCCAGTGCTGTAGAGTCGGAAACAGGCATGGGGAGATTGCCAGGCTGACCCTCTCTGGTCTGTTTCCAG
  GCGTCTCACTTCTTCTGGGCTCTCTGGGCCCTCATCCAGAACCAGTACTCCACCATCGACTT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG214974 representing NM_018208
  Red=Cloning site Green=Tags(s)
  
  MAVPPSAPQQRASFHLRRHTPCPQCSWGMEEKAAASASCREPPGPPRAAAVAYFGISVDPDDILPGALRL
  IQELRPHWKPEQVRTKRFTDGITNKLVACYVEEDMQDCVLVRVYGERTELLVDRENEVRNFQLLRAHSCA
  PKLYCTFQNGLCYEYMQGVALEPEHIREPRLFRLIALEMAKIHTIHANGSLPKPILWHKMHNYFTLVKNE
  INPSLSADVPKVEVLERELAWLKEHLSQLESPVVFCHNDLLCKNIIYDSIKGHVRFIDYEYAGYNYQAFD
  IGNHFNEFAGVNEVDYCLYPARETQLQWLHYYLQAQKGMAVTPREVQRLYVQVNKFALGPSCVSSTMTAS
  LQCCRVGNRHGEIARLTLSGLFPGVSLLLGSLGPHPEPVLHHRL
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_018208
• ORF Size: 1182 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_018208.1, NP_060678.1
• RefSeq Size: 2430
• RefSeq ORF: 1161
• Locus ID: 55224
• Domains: Choline_kinase
• Protein Families: Druggable Genome
• Protein Pathways: Glycerophospholipid metabolism, Metabolic pathways
• Gene Summary: The protein encoded by this gene is a member of choline/ethanolamine kinase family which catalyzes the first step of phosphatidylethanolamine (PtdEtn) biosynthesis via the cytidine diphosphate (CDP) ethanolamine pathway. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]