CA7 (NM_005182) Human Tagged ORF Clone

CAT#: RG215024

CA7 (GFP-tagged) - Human carbonic anhydrase VII (CA7), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: CA7
• Synonyms: CA-VII; CAVII
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG215024 representing NM_005182
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCGGCCACCACGGCTGGGGCTACGGCCAGGACGACGGCCCCTCGCATTGGCACAAGCTGTATCCCA
  TTGCCCAGGGAGATCGCCAATCACCCATCAATATCATCTCCAGCCAGGCTGTGTACTCTCCCAGCCTGCA
  ACCACTGGAGCTTTCCTATGAGGCCTGCATGTCCCTCAGCATCACCAACAATGGCCACTCTGTCCAGGTA
  GACTTCAATGACAGCGATGACCGAACCGTGGTGACTGGGGGCCCCCTGGAAGGGCCCTACCGCCTCAAGC
  AGTTTCACTTCCACTGGGGCAAGAAGCACGATGTGGGTTCTGAGCACACGGTGGACGGCAAGTCCTTCCC
  CAGCGAGCTGCATCTGGTTCACTGGAATGCCAAGAAGTACAGCACTTTTGGGGAGGCGGCCTCAGCACCT
  GATGGCCTGGCTGTGGTTGGTGTTTTTTTGGAGACAGGAGACGAGCACCCCAGCATGAATCGTCTGACAG
  ATGCGCTCTACATGGTCCGGTTCAAGGGCACCAAAGCCCAGTTCAGCTGCTTCAACCCCAAGTGCCTCCT
  GCCTGCCAGCCGGCACTACTGGACCTACCCGGGCTCTCTGACGACTCCCCCACTCAGTGAGAGTGTCACC
  TGGATTGTGCTCCGGGAGCCCATCTGCATCTCTGAAAGGCAGATGGGGAAGTTCCGGAGCCTGCTTTTTA
  CCTCGGAGGACGATGAGAGGATCCACATGGTGAACAACTTCCGGCCACCACAGCCACTGAAGGGCCGCGT
  GGTAAAGGCCTCCTTCCGGGCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG215024 representing NM_005182
  Red=Cloning site Green=Tags(s)
  
  MTGHHGWGYGQDDGPSHWHKLYPIAQGDRQSPINIISSQAVYSPSLQPLELSYEACMSLSITNNGHSVQV
  DFNDSDDRTVVTGGPLEGPYRLKQFHFHWGKKHDVGSEHTVDGKSFPSELHLVHWNAKKYSTFGEAASAP
  DGLAVVGVFLETGDEHPSMNRLTDALYMVRFKGTKAQFSCFNPKCLLPASRHYWTYPGSLTTPPLSESVT
  WIVLREPICISERQMGKFRSLLFTSEDDERIHMVNNFRPPQPLKGRVVKASFRA
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005182
• ORF Size: 792 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005182.2, NP_005173.1
• RefSeq Size: 1563 bp
• RefSeq ORF: 795 bp
• Locus ID: 766
• Cytogenetics: 16q22.1
• Protein Families: Druggable Genome
• Protein Pathways: Nitrogen metabolism
• Gene Summary: 'Carbonic anhydrases are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. The cytosolic protein encoded by this gene is predominantly expressed in the brain and contributes to bicarbonate driven GABAergic neuron excitation. Alternative splicing in the coding region results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Aug 2018]'