ABLIM1 (NM_006720) Human Tagged ORF Clone

CAT#: RG215049

ABLIM1 (GFP-tagged) - Human actin binding LIM protein 1 (ABLIM1), transcript variant 4

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ABLIM1
• Synonyms: ABLIM; abLIM-1; LIMAB1; LIMATIN
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG215049 representing NM_006720
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTCACAGAAGGAGAGGAAATGTATCTTCAAGGCTCCACCGTTTGGCATCCCGACTGTAAGCAATCTA
  CGAAGACCGAGGAAAAGCTGCGGCTGCCAAACATTCGGCGTTCTTCATCCGATTTCTTTTATTCCAAAAG
  TCTGATTCGACGCACAGGACGGTCTCCGTCTCTGCAGCCTACCAGGACATCCTCGGAAAGTATTTATTCT
  AGGCCAGGCTCCAGTATTCCTGGCTCACCAGGTCATACTATCTATGCAAAAGTAGACAATGAGATCCTGG
  ATTACAAGGATTTAGCAGCCATTCCGAAGGTCAAGGCAATTTATGACATTGAACGTCCAGATCTTATTAC
  CTATGAGCCTTTCTACACTTCGGGCTATGATGACAAACAGGAGAGACAGAGCCTTGGAGAGTCTCCGAGG
  ACTTTGTCTCCTACTCCATCAGCAGAAGGGTACCAGGATGTTCGGGATCGGATGATCCATCGGTCCACGA
  GCCAGGGCTCCATCAACTCCCCTGTGTACAGCCGCCACAGCTACACTCCAACCACGTCCCGCTCTCCCCA
  GCATTTCCACAGACCTGATCAAGGAATCAACATTTACCGAAAGCCACCCATCTACAAACAGCATGCTGCC
  TTGGCAGCCCAGAGCAAGTCCTCAGAAGATATCATCAAGTTTTCCAAGTTCCCAGCAGCCCAGGCACCAG
  ACCCCAGCGAGACACCAAAGATTGAGACGGACCACTGGCCTGGTCCCCCCTCATTTGCTGTCGTAGGACC
  TGACATGAAACGCAGATCTAGTGGCAGAGAGGAAGATGATGAGGAACTTCTGAGACGTCGGCAGCTTCAA
  GAAGAGCAATTAATGAAGCTTAACTCAGGCCTGGGACAGTTGATCTTGAAAGAAGAGATGGAGAAAGAGA
  GCCGGGAAAGGTCATCTCTGTTAGCCAGTCGCTACGATTCTCCCATCAACTCAGCTTCACATATTCCATC
  ATCTAAAACTGCATCTCTCCCTGGCTATGGAAGAAATGGGCTTCACCGGCCTGTTTCTACCGACTTCGCT
  CAGTATAACAGCTATGGGGATGTCAGCGGGGGAGTGCGAGATTACCAGACACTCCCAGATGGCCACATGC
  CTGCAATGAGAATGGACCGAGGAGTGTCTATGCCCAACATGTTGGAACCAAAGATATTTCCATATGAAAT
  GCTCATGGTGACCAACAGAGGGCGAAACAAAATCCTCAGAGAGGTGGACAGAACCAGGCTGGAGCGCCAC
  TTAGCCCCTGAAGTGTTTCGGGAAATCTTTGGAATGTCCATACAGGAGTTTGACAGGTTACCTCTTTGGA
  GACGCAACGACATGAAGAAAAAAGCAAAACTCTTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG215049 representing NM_006720
  Red=Cloning site Green=Tags(s)
  
  MFTEGEEMYLQGSTVWHPDCKQSTKTEEKLRLPNIRRSSSDFFYSKSLIRRTGRSPSLQPTRTSSESIYS
  RPGSSIPGSPGHTIYAKVDNEILDYKDLAAIPKVKAIYDIERPDLITYEPFYTSGYDDKQERQSLGESPR
  TLSPTPSAEGYQDVRDRMIHRSTSQGSINSPVYSRHSYTPTTSRSPQHFHRPDQGINIYRKPPIYKQHAA
  LAAQSKSSEDIIKFSKFPAAQAPDPSETPKIETDHWPGPPSFAVVGPDMKRRSSGREEDDEELLRRRQLQ
  EEQLMKLNSGLGQLILKEEMEKESRERSSLLASRYDSPINSASHIPSSKTASLPGYGRNGLHRPVSTDFA
  QYNSYGDVSGGVRDYQTLPDGHMPAMRMDRGVSMPNMLEPKIFPYEMLMVTNRGRNKILREVDRTRLERH
  LAPEVFREIFGMSIQEFDRLPLWRRNDMKKKAKLF
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006720
• ORF Size: 1365 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006720.3, NP_006711.3
• RefSeq Size: 6850 bp
• RefSeq ORF: 1368 bp
• Locus ID: 3983
• Cytogenetics: 10q25.3
• Domains: VHP
• Protein Pathways: Axon guidance
• Gene Summary: 'This gene encodes a LIM zinc-binding domain-containing protein that binds to actin filaments and mediates interactions between actin and cytoplasmic targets. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jun 2017]'