ACCN4 (ASIC4) (NM_018674) Human Tagged ORF Clone

CAT#: RG215051

GFP-tagged ORF clone of Homo sapiens amiloride-sensitive cation channel 4, pituitary (ACCN4), transcript variant 1 as transfection-ready DNA

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ASIC4
• Synonyms: ACCN4; BNAC4
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG215051 representing NM_018674
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGAGCGGAGCGGCTGGGGCTGCGCGGCGTGGCGGAGCAGCGCTCGCTCCCTCGCTCACTCGCTCGC
  TCGCAGGGACACACGCAGGGGCTGACAGCTGTGCTGGTGCTGATAAGGGAAGCCACAAGGAGACGATCGA
  GGAGAGAGACAAGCGGCAGCAGAGGCAGCAGCGGCAGAGGCAGCACCAGGGCTGCGGAGCTGCTGGGAGT
  GGGAGTGACTCCCCCACCTCGGGCCCCCACCCTGTCCCTGTCCTCTTCCCGCTTGCCCTGAGTTTAGAAG
  AGCAGCCGCTGCCACCACTGCCACTCGGGAGGGCACCAGGGCTGCTGGCTAGGGAGGGACAGGGCAGGGA
  GGCTCTGGCCAGTCCCAGCAGCCGGGGACAGATGCCGATCGAGATTGTGTGCAAAATCAAATTTGCTGAG
  GAGGATGCGAAACCCAAGGAGAAGGAGGCAGGGGATGAGCAGAGCCTCCTCGGGGCTGTTGCCCCTGGAG
  CAGCCCCCCGAGACCTGGCCACCTTTGCCAGCACCAGCACCCTGCATGGACTGGGCCGGGCCTGTGGCCC
  AGGCCCCCACGGACTGCGCAGAACCCTGTGGGCACTGGCCCTACTCACCTCGCTGGCTGCCTTCCTGTAC
  CAGGCGGCTGGCCTGGCCCGGGGCTACCTGACCCGGCCTCACCTGGTGGCAATGGACCCCGCTGCCCCAG
  CCCCAGTGGCGGGCTTCCCGGCTGTCACCCTCTGCAATATCAACCGCTTCCGGCATTCGGCACTCAGCGA
  TGCCGACATCTTCCACCTGGCCAATCTGACAGGGCTGCCCCCCAAAGACCGGGATGGGCACCGTGCGGCT
  GGCCTGCGCTACCCAGAGCCTGACATGGTAGACATCCTCAACCGCACTGGCCACCAGCTCGCCGACATGC
  TTAAGAGCTGCAACTTCAGTGGGCATCACTGCTCCGCCAGCAACTTCTCTGTGGTCTATACTCGCTATGG
  GAAGTGTTACACCTTCAACGCGGACCCGCGGAGCTCGCTGCCCAGCCGGGCAGGGGGCATGGGCAGTGGC
  CTGGAGATCATGCTGGACATCCAGCAGGAGGAGTACCTGCCCATCTGGAGGGAGACAAATGAGACGTCGT
  TTGAGGCAGGTATTCGGGTGCAGATCCACAGCCAGGAGGAGCCGCCCTACATCCACCAGCTGGGGTTCGG
  GGTGTCCCCAGGCTTCCAGACCTTTGTGTCCTGCCAGGAACAGCGGCTGACCTACCTGCCCCAGCCCTGG
  GGCAACTGCCGCGCAGAGAGTGAGCTCAGGGAGCCTGAGCTTCAGGGCTACTCGGCCTACAGTGTGTCTG
  CCTGCCGGCTGCGCTGTGAAAAGGAGGCCGTGCTTCAGCGCTGCCACTGCCGGATGGTGCACATGCCAGA
  CTCCCTGGGTGGGGGCCCTGAGGGCCCGTGCTTCTGCCCCACCCCCTGCAACCTGACACGCTATGGGAAA
  GAGATCTCCATGGTCAGGATCCCCAACAGGGGCTCAGCCCGGTACCTGGCGAGGAAGTACAACCGCAACG
  AGACCTACATACGGGAGAACTTCCTGGTCCTAGATGTCTTCTTTGAGGCCCTGACCTCTGAAGCCATGGA
  GCAGCGAGCAGCCTATGGCCTGTCAGCCCTGCTGGGAGACCTCGGGGGACAGATGGGCCTGTTCATTGGG
  GCCAGCATCCTCACGTTGCTGGAGATCCTCGACTACATCTATGAGGTGTCCTGGGATCGACTGAAGCGGG
  TATGGAGGCGTCCCAAGACCCCCCTGCGGACCTCCACTGGGGGCATCTCCACTTTGGGGCTTCAGGAGCT
  GAAGGAACAGAGTCCCTGCCCGAGCCTGGGCCGAGCGGAGGGTGGGGGGGTCAGCAGTCTGCTCCCCAAT
  CACCACCACCCCCACGGTCCCCCAGGAGGTCTCTTTGAAGATTTTGCTTGC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG215051 representing NM_018674
  Red=Cloning site Green=Tags(s)
  
  MLSGAAGAARRGGAALAPSLTRSLAGTHAGADSCAGADKGSHKETIEERDKRQQRQQRQRQHQGCGAAGS
  GSDSPTSGPHPVPVLFPLALSLEEQPLPPLPLGRAPGLLAREGQGREALASPSSRGQMPIEIVCKIKFAE
  EDAKPKEKEAGDEQSLLGAVAPGAAPRDLATFASTSTLHGLGRACGPGPHGLRRTLWALALLTSLAAFLY
  QAAGLARGYLTRPHLVAMDPAAPAPVAGFPAVTLCNINRFRHSALSDADIFHLANLTGLPPKDRDGHRAA
  GLRYPEPDMVDILNRTGHQLADMLKSCNFSGHHCSASNFSVVYTRYGKCYTFNADPRSSLPSRAGGMGSG
  LEIMLDIQQEEYLPIWRETNETSFEAGIRVQIHSQEEPPYIHQLGFGVSPGFQTFVSCQEQRLTYLPQPW
  GNCRAESELREPELQGYSAYSVSACRLRCEKEAVLQRCHCRMVHMPDSLGGGPEGPCFCPTPCNLTRYGK
  EISMVRIPNRGSARYLARKYNRNETYIRENFLVLDVFFEALTSEAMEQRAAYGLSALLGDLGGQMGLFIG
  ASILTLLEILDYIYEVSWDRLKRVWRRPKTPLRTSTGGISTLGLQELKEQSPCPSLGRAEGGGVSSLLPN
  HHHPHGPPGGLFEDFAC
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_018674
• ORF Size: 1998 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_018674.3, NP_061144.2
• RefSeq Size: 2754
• RefSeq ORF: 2001
• Locus ID: 55515
• Protein Families: Druggable Genome, Ion Channels: Other
• Gene Summary: This gene belongs to the superfamily of acid-sensing ion channels, which are proton-gated, amiloride-sensitive sodium channels. These channels have been implicated in synaptic transmission, pain perception as well as mechanoperception. This gene is predominantly expressed in the pituitary gland, and was considered a candidate for paroxysmal dystonic choreoathetosis (PDC), a movement disorder, however, no correlation was found between mutations in this gene and PDC. [provided by RefSeq, Feb 2012]