VILL (NM_015873) Human Tagged ORF Clone

CAT#: RG215217

VILL (GFP-tagged) - Human villin-like (VILL)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: VILL
• Synonyms: OTTHUMP00000161384; villin-like; villin-like protein
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG215217 representing NM_015873
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACATCAGCAAGGGCCTCCCAGGCATGCAGGGAGGCCTCCACATATGGATCTCTGAGAACCGGAAGA
  TGGTGCCGGTACCCGAGGGGGCTTACGGGAACTTTTTTGAGGAACACTGCTATGTCATCCTCCACGTCCC
  CCAGAGCCCGAAGGCCACGCAGGGGGCGTCCAGCGACCTGCACTACTGGGTCGGGAAGCAGGCGGGTGCG
  GAAGCGCAGGGCGCTGCGGAGGCCTTCCAGCAGCGCCTACAGGACGAGCTGGGGGGCCAGACCGTGCTGC
  ACCGCGAGGCGCAGGGCCACGAGTCCGACTGCTTCTGCAGCTACTTCCGCCCGGGAATCATCTACAGGAA
  GGGAGGCCTAGCATCTGACCTCAAGCATGTGGAGACCAACTTGTTCAACATCCAGCGACTGCTGCACATC
  AAAGGGAGGAAGCACGTGTCTGCCACTGAGGTGGAGCTCTCCTGGAACAGCTTTAATAAGGGTGACATCT
  TCCTGCTGGACCTAGGCAAGATGATGATTCAGTGGAATGGGCCCAAGACCAGCATTTCTGAGAAGGCTCG
  GGGGCTGGCTTTGACCTACAGCCTCCGGGACAGGGAACGTGGTGGTGGTCGTGCACAGATTGGTGTGGTG
  GATGATGAGGCCAAAGCCCCGGACCTCATGCAGATCATGGAGGCTGTGCTGGGCCGCAGGGTGGGCAGCC
  TGCGTGCCGCCACGCCCAGCAAGGATATCAACCAGCTGCAGAAGGCCAATGTTCGCCTGTACCATGTCTA
  TGAGAAGGGCAAAGACCTGGTGGTCCTGGAGTTGGCGACCCCCCCACTGACCCAGGACCTGCTGCAGGAG
  GAGGACTTCTACATCCTGGACCAGGGTGGCTTCAAGATCTATGTGTGGCAGGGACGCATGTCTAGCCTCC
  AGGAGAGAAAGGCTGCCTTCAGCCGGGCTGTGGGCTTCATCCAGGCCAAGGGCTACCCGACCTACACCAA
  CGTGGAGGTGGTGAACGACGGCGCCGAGTCGGCCGCGTTCAAGCAGCTCTTCCGGACTTGGTCTGAGAAG
  CGGCGCAGGAACCAGAAGCTCGGCGGGAGGGATAAATCGATTCATGTAAAGCTGGACGTGGGCAAGCTGC
  ACACCCAGCCTAAGTTAGCGGCCCAGCTCAGGATGGTGGACGACGGCTCTGGGAAGGTGGAGGTGTGGTG
  CATCCAGGACTTACACAGGCAGCCCGTGGACCCCAAGCGTCATGGACAGCTGTGTGCAGGCAACTGCTAC
  CTTGTGCTCTACACATACCAGAGGCTGGGCCGTGTCCAGTACATCCTGTACCTATGGCAGGGCCACCAGG
  CCACTGCGGATGAGATTGAGGCCCTGAACAGCAACGCTGAGGAACTAGATGTCATGTATGGTGGCGTCCT
  AGTACAGGAGCATGTGACCATGGGCAGCGAGCCCCCCCACTTCCTCGCCATCTTCCAGGGCCAGCTGGTG
  ATCTTCCAGGAGAGAGCTGGGCACCATGGAAAGGGGCAGTCAGCATCCACCACAAGGCTTTTCCAAGTGC
  AAGGCACTGACAGCCACAACACCAGGACCATGGAGGTGCCAGCCCGTGCCTCATCCCTCAACTCCAGTGA
  CATCTTCTTGCTGGTCACAGCCAGCGTCTGCTACCTCTGGTTTGGGAAGGGCTGTAATGGTGATCAGCGT
  GAGATGGCACGGGTGGTGGTCACTGTCATTTCCAGGAAGAATGAGGAAACGGTGCTGGAGGGTCAGGAGC
  CTCCCCACTTCTGGGAGGCCCTGGGAGGCCGGGCCCCCTACCCCAGCAACAAGAGGCTCCCTGAGGAGGT
  CCCCAGCTTCCAGCCACGACTGTTTGAGTGCTCCAGCCACATGGGCTGCCTGGTCCTCGCAGAAGTGGGG
  TTCTTCAGCCAGGAGGACCTGGACAAGTATGACATCATGTTACTGGACACCTGGCAGGAGATCTTCCTGT
  GGCTTGGGGAAGCTGCAAGTGAGTGGAAGGAGGCGGTGGCCTGGGGCCAGGAGTACCTGAAGACTCACCC
  AGCAGGGAGGAGCCCGGCCACACCCATCGTGCTGGTCAAGCAGGGCCATGAGCCTCCCACCTTCATTGGA
  TGGTTCTTCACTTGGGACCCCTACAAGTGGACTAGCCACCCGTCCCACAAGGAAGTGGTGGATGGCAGCC
  CGGCAGCAGCATCAACCATCTCTGAGATAACAGCAGAAGTCAACAACTTGCGGCTATCCAGATGGCCGGG
  CAATGGCAGGGCAGGTGCCGTGGCCCTGCAGGCCCTCAAGGGCTCCCAGGACAGCTCAGAGAATGATCTG
  GTGCGAAGCCCCAAGTCGGCTGGCAGCAGAACCAGCAGCTCCGTCAGCAGCACCAGCGCCACGATCAACG
  GGGGCCTGCGCCGGGAACAACTGATGCACCAGGCTGTTGAGGACCTGCCAGAGGGCGTGGACCCTGCCCG
  CAGGGAGTTCTATCTCTCAGACTCTGACTTCCAAGATATCTTTGGGAAATCCAAGGAGGAATTCTACAGC
  ATGGCCACGTGGAGGCAGCGGCAGGAGAAAAAGCAGCTGGGCTTCTTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG215217 representing NM_015873
  Red=Cloning site Green=Tags(s)
  
  MDISKGLPGMQGGLHIWISENRKMVPVPEGAYGNFFEEHCYVILHVPQSPKATQGASSDLHYWVGKQAGA
  EAQGAAEAFQQRLQDELGGQTVLHREAQGHESDCFCSYFRPGIIYRKGGLASDLKHVETNLFNIQRLLHI
  KGRKHVSATEVELSWNSFNKGDIFLLDLGKMMIQWNGPKTSISEKARGLALTYSLRDRERGGGRAQIGVV
  DDEAKAPDLMQIMEAVLGRRVGSLRAATPSKDINQLQKANVRLYHVYEKGKDLVVLELATPPLTQDLLQE
  EDFYILDQGGFKIYVWQGRMSSLQERKAAFSRAVGFIQAKGYPTYTNVEVVNDGAESAAFKQLFRTWSEK
  RRRNQKLGGRDKSIHVKLDVGKLHTQPKLAAQLRMVDDGSGKVEVWCIQDLHRQPVDPKRHGQLCAGNCY
  LVLYTYQRLGRVQYILYLWQGHQATADEIEALNSNAEELDVMYGGVLVQEHVTMGSEPPHFLAIFQGQLV
  IFQERAGHHGKGQSASTTRLFQVQGTDSHNTRTMEVPARASSLNSSDIFLLVTASVCYLWFGKGCNGDQR
  EMARVVVTVISRKNEETVLEGQEPPHFWEALGGRAPYPSNKRLPEEVPSFQPRLFECSSHMGCLVLAEVG
  FFSQEDLDKYDIMLLDTWQEIFLWLGEAASEWKEAVAWGQEYLKTHPAGRSPATPIVLVKQGHEPPTFIG
  WFFTWDPYKWTSHPSHKEVVDGSPAAASTISEITAEVNNLRLSRWPGNGRAGAVALQALKGSQDSSENDL
  VRSPKSAGSRTSSSVSSTSATINGGLRREQLMHQAVEDLPEGVDPARREFYLSDSDFQDIFGKSKEEFYS
  MATWRQRQEKKQLGFF
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015873
• ORF Size: 2568 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_015873.3, NP_056957.3
• RefSeq Size: 2787
• RefSeq ORF: 2571
• Locus ID: 50853
• Domains: VHP, GEL, Gelsolin
• Gene Summary: The protein encoded by this gene belongs to the villin/gelsolin family. It contains 6 gelsolin-like repeats and a headpiece domain. It may play a role in actin-bundling. [provided by RefSeq, Jul 2008]