GABA B Receptor 1 (GABBR1) (NM_021904) Human Tagged ORF Clone

CAT#: RG215515

GABBR1 (GFP-tagged) - Human gamma-aminobutyric acid (GABA) B receptor, 1 (GABBR1), transcript variant 3

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: GABBR1
• Synonyms: GABABR1; GABBR1-3; GB1; GPRC3A
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG215515 representing NM_021904
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTGCTGCTGCTGCTACTGGCGCCACTCTTCCTCCGCCCCCCGGGCGCGGGCGGGGCGCATACCCCCA
  ACGCCACCTCAGAAGGTTGCCAGATCATACACCCGCCCTGGGAAGGGGGCATCAGGTACCGGGGCCTGAC
  TCGGGACCAGGTGAAGGCTATCAACTTCCTGCCAGTGGACTATGAGATTGAGTATGTGTGCCGGGGGGAG
  CGCGAGGTGGTGGGGCCCAAGGTCCGCAAGTGCCTGGCCAACGGCTCCTGGACAGATATGGACACACCCA
  GCCGCTGTGTGAATCGAACGCCACACTCAGAACGGCGCGCAGTGTACATCGGGGCACTGTTTCCCATGAG
  CGGGGGCTGGCCAGGGGGCCAGGCCTGCCAGCCCGCGGTGGAGATGGCGCTGGAGGACGTGAATAGCCGC
  AGGGACATCCTGCCGGACTATGAGCTCAAGCTCATCCACCACGACAGCAAGTGTGATCCAGGCCAAGCCA
  CCAAGTACCTATATGAGCTGCTCTACAACGACCCTATCAAGATCATCCTTATGCCTGGCTGCAGCTCTGT
  CTCCACGCTGGTGGCTGAGGCTGCTAGGATGTGGAACCTCATTGTGCTTTCCTATGGCTCCAGCTCACCA
  GCCCTGTCAAACCGGCAGCGTTTCCCCACTTTCTTCCGAACGCACCCATCAGCCACACTCCACAACCCTA
  CCCGCGTGAAACTCTTTGAAAAGTGGGGCTGGAAGAAGATTGCTACCATCCAGCAGACCACTGAGGTCTT
  CACTTCGACTCTGGACGACCTGGAGGAACGAGTGAAGGAGGCTGGAATTGAGATTACTTTCCGCCAGAGT
  TTCTTCTCAGATCCAGCTGTGCCCGTCAAAAACCTGAAGCGCCAGGATGCCCGAATCATCGTGGGACTTT
  TCTATGAGACTGAAGCCCGGAAAGTTTTTTGTGAGGTGTACAAGGAGCGTCTCTTTGGGAAGAAGTACGT
  CTGGTTCCTCATTGGGTGGTATGCTGACAATTGGTTCAAGATCTACGACCCTTCTATCAACTGCACAGTG
  GATGAGATGACTGAGGCGGTGGAGGGCCACATCACAACTGAGATTGTCATGCTGAATCCTGCCAATACCC
  GCAGCATTTCCAACATGACATCCCAGGAATTTGTGGAGAAACTAACCAAGCGACTGAAAAGACACCCTGA
  GGAGACAGGAGGCTTCCAGGAGGCACCGCTGGCCTATGATGCCATCTGGGCCTTGGCACTGGCCCTGAAC
  AAGACATCTGGAGGAGGCGGCCGTTCTGGTGTGCGCCTGGAGGACTTCAACTACAACAACCAGACCATTA
  CCGACCAAATCTACCGGGCAATGAACTCTTCGTCCTTTGAGGGTGTCTCTGGCCATGTGGTGTTTGATGC
  CAGCGGCTCTCGGATGGCATGGACGCTTATCGAGCAGCTTCAGGGTGGCAGCTACAAGAAGATTGGCTAC
  TATGACAGCACCAAGGATGATCTTTCCTGGTCCAAAACAGATAAATGGATTGGAGGGTCCCCCCCAGCTG
  ACCAGACCCTGGTCATCAAGACATTCCGCTTCCTGTCACAGAAACTCTTTATCTCCGTCTCAGTTCTCTC
  CAGCCTGGGCATTGTCCTAGCTGTTGTCTGTCTGTCCTTTAACATCTACAACTCACATGTCCGTTATATC
  CAGAACTCACAGCCCAACCTGAACAACCTGACTGCTGTGGGCTGCTCACTGGCTTTAGCTGCTGTCTTCC
  CCCTGGGGCTCGATGGTTACCACATTGGGAGGAACCAGTTTCCTTTCGTCTGCCAGGCCCGCCTCTGGCT
  CCTGGGCCTGGGCTTTAGTCTGGGCTACGGTTCCATGTTCACCAAGATTTGGTGGGTCCACACGGTCTTC
  ACAAAGAAGGAAGAAAAGAAGGAGTGGAGGAAGACTCTGGAACCCTGGAAGCTGTATGCCACAGTGGGCC
  TGCTGGTGGGCATGGATGTCCTCACTCTCGCCATCTGGCAGATCGTGGACCCTCTGCACCGGACCATTGA
  GACATTTGCCAAGGAGGAACCTAAGGAAGATATTGACGTCTCTATTCTGCCCCAGCTGGAGCATTGCAGC
  TCCAGGAAGATGAATACATGGCTTGGCATTTTCTATGGTTACAAGGGGCTGCTGCTGCTGCTGGGAATCT
  TCCTTGCTTATGAGACCAAGAGTGTGTCCACTGAGAAGATCAATGATCACCGGGCTGTGGGCATGGCTAT
  CTACAATGTGGCAGTCCTGTGCCTCATCACTGCTCCTGTCACCATGATTCTGTCCAGCCAGCAGGATGCA
  GCCTTTGCCTTTGCCTCTCTTGCCATAGTTTTCTCCTCCTATATCACTCTTGTTGTGCTCTTTGTGCCCA
  AGATGCGCAGGCTGATCACCCGAGGGGAATGGCAGTCGGAGGCGCAGGACACCATGAAGACAGGGTCATC
  GACCAACAACAACGAGGAGGAGAAGTCCCGGCTGTTGGAGAAGGAGAACCGTGAACTGGAAAAGATCATT
  GCTGAGAAAGAGGAGCGTGTCTCTGAACTGCGCCATCAACTCCAGTCTCGGCAGCAGCTCCGCTCCCGGC
  GCCACCCACCGACACCCCCAGAACCCTCTGGGGGCCTGCCCAGGGGACCCCCTGAGCCCCCCGACCGGCT
  TAGCTGTGATGGGAGTCGAGTGCATTTGCTTTATAAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG215515 representing NM_021904
  Red=Cloning site Green=Tags(s)
  
  MLLLLLLAPLFLRPPGAGGAHTPNATSEGCQIIHPPWEGGIRYRGLTRDQVKAINFLPVDYEIEYVCRGE
  REVVGPKVRKCLANGSWTDMDTPSRCVNRTPHSERRAVYIGALFPMSGGWPGGQACQPAVEMALEDVNSR
  RDILPDYELKLIHHDSKCDPGQATKYLYELLYNDPIKIILMPGCSSVSTLVAEAARMWNLIVLSYGSSSP
  ALSNRQRFPTFFRTHPSATLHNPTRVKLFEKWGWKKIATIQQTTEVFTSTLDDLEERVKEAGIEITFRQS
  FFSDPAVPVKNLKRQDARIIVGLFYETEARKVFCEVYKERLFGKKYVWFLIGWYADNWFKIYDPSINCTV
  DEMTEAVEGHITTEIVMLNPANTRSISNMTSQEFVEKLTKRLKRHPEETGGFQEAPLAYDAIWALALALN
  KTSGGGGRSGVRLEDFNYNNQTITDQIYRAMNSSSFEGVSGHVVFDASGSRMAWTLIEQLQGGSYKKIGY
  YDSTKDDLSWSKTDKWIGGSPPADQTLVIKTFRFLSQKLFISVSVLSSLGIVLAVVCLSFNIYNSHVRYI
  QNSQPNLNNLTAVGCSLALAAVFPLGLDGYHIGRNQFPFVCQARLWLLGLGFSLGYGSMFTKIWWVHTVF
  TKKEEKKEWRKTLEPWKLYATVGLLVGMDVLTLAIWQIVDPLHRTIETFAKEEPKEDIDVSILPQLEHCS
  SRKMNTWLGIFYGYKGLLLLLGIFLAYETKSVSTEKINDHRAVGMAIYNVAVLCLITAPVTMILSSQQDA
  AFAFASLAIVFSSYITLVVLFVPKMRRLITRGEWQSEAQDTMKTGSSTNNNEEEKSRLLEKENRELEKII
  AEKEERVSELRHQLQSRQQLRSRRHPPTPPEPSGGLPRGPPEPPDRLSCDGSRVHLLYK
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_021904
• ORF Size: 2697 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021904.1, NP_068704.1
• RefSeq Size: 4260 bp
• RefSeq ORF: 2700 bp
• Locus ID: 2550
• Cytogenetics: 6p22.1
• Domains: 7tm_3, ANF_receptor
• Protein Families: Druggable Genome, GPCR, Secreted Protein, Transmembrane
• Protein Pathways: Neuroactive ligand-receptor interaction
• Gene Summary: 'This gene encodes a receptor for gamma-aminobutyric acid (GABA), which is the main inhibitory neurotransmitter in the mammalian central nervous system. This receptor functions as a heterodimer with GABA(B) receptor 2. Defects in this gene may underlie brain disorders such as schizophrenia and epilepsy. Alternative splicing generates multiple transcript variants, but the full-length nature of some of these variants has not been determined. [provided by RefSeq, Jan 2016]'