ARHGEF4 (NM_015320) Human Tagged ORF Clone

CAT#: RG215591

ARHGEF4 (GFP-tagged) - Human Rho guanine nucleotide exchange factor (GEF) 4 (ARHGEF4), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ARHGEF4
• Synonyms: ASEF; ASEF1; GEF4; SMIM39; STM6
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG215591 representing NM_015320
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGTCTCCACTACCCCGGGAGGGGTAGCGCCATCTCCATGGTTTCTCTTGGAAGCTACAGCTACGTGG
  ACAGCAGTTCAGGGGACCCTGAAAGACCCAAGATTCCCAAGGGCCAGACCAGTTTCCTGCTTTCTCTGCA
  GACGCTAAACCAAGATGAGCAGAAGGAAGAGAGCAGGGAAGGAGGCCAGGGTCCGCGCGGCTTGGGCACA
  GTGCCCTGGCTCAGGGACCTTCCTGGGAGTGAGAACCACATGCCCTGGGAAGAACCAGCAGGTGAGAAGC
  CCAGTTGCTCTCACAGTCAGAAGGCATTCCACATGGAGCCTGCCCAGAAGCCCTGCTTCACCACTGACAT
  GGTGACATGGGCCCTCCTCTGCATCTCTGCAGAGACTGTGCGTGGGGAGGCTCCTTCACAGCCTAGGGGC
  ATCCCTCACCGCTCGCCCGTCAGTGTGGATGACCTGTGGCTGGAGAAGACACAGAGAAAGAAGTTGCAGA
  AGCAGGCCCACATCGAAAGGAGGCTGCACATAGGGGCAGTGCACAAAGATGGAGTCAAGTGCTGGAGAAA
  GACGATCATTACCTCTCCAGAGTCTTTGAATCTCCCTAGAAGAAGCCATCCACTCTCCCAGAGTGCTCCA
  ACGGGACTGAACCACATGGGCTGGCCAGAGCACACACCAGGCACTGCCATGCCTGATGGAGCTCTGGACA
  CAGCTGTCTGCGCTGACGAAGTGGGGAGCGAGGAGGACCTGTATGATGACCTGCACAGCTCCAGCCACCA
  CTACAGCCACCCTGGAGGGGGTGGGGAGCAGCTGGCTATCAATGAGCTCATCAGCGATGGCAGTGTGGTC
  TGCGCTGAAGCACTCTGGGACCATGTCACCATGGACGACCAGGAGCTGGGCTTCAAAGCTGGGGACGTCA
  TCGAAGTGATGGATGCCACCAACAGAGAGTGGTGGTGGGGCCGGGTCGCCGATGGCGAGGGCTGGTTTCC
  AGCCAGCTTCGTTCGGCTGAGGGTGAATCAGGACGAGCCCGCGGATGACGACGCCCCTCTGGCCGGGAAC
  AGCGGAGCGGAGGACGGCGGGGCGGAGGCGCAGAGCAGCAAGGACCAGATGCGGACCAACGTCATCAACG
  AGATCCTCAGCACTGAGCGGGACTACATCAAGCACCTGCGCGACATCTGCGAGGGCTACGTCCGGCAGTG
  CCGCAAGCGCGCAGACATGTTCAGCGAGGAGCAGCTGCGTACCATCTTCGGGAACATCGAGGACATCTAC
  CGCTGCCAGAAGGCCTTCGTGAAGGCCCTGGAGCAGAGGTTCAACCGCGAGCGCCCACACCTGAGCGAGC
  TGGGTGCCTGCTTCCTGGAGCATCAAGCCGACTTCCAGATCTACTCGGAGTACTGCAATAACCACCCCAA
  CGCCTGCGTGGAGCTCTCCCGGCTCACCAAGCTCAGCAAGTACGTGTACTTCTTCGAGGCCTGCCGGCTG
  CTGCAGAAGATGATTGACATCTCCCTGGATGGCTTCCTGCTGACTCCGGTGCAGAAGATCTGCAAGTACC
  CTCTGCAGCTGGCCGAGCTGCTCAAATACACGCACCCCCAGCACAGGGACTTCAAGGATGTTGAAGCCGC
  CTTGCATGCCATGAAGAACGTGGCCCAGCTCATCAACGAGCGGAAGCGGAGACTTGAGAACATCGACAAG
  ATTGCTCAGTGGCAGAGCTCCATAGAGGACTGGGAGGGAGAAGATCTCTTGGTCAGGAGCTCAGAACTCA
  TCTACTCGGGGGAGCTGACTCGAGTTACACAGCCTCAAGCCAAAAGCCAGCAGCGAATGTTCTTTCTCTT
  TGACCACCAGCTCATCTACTGTAAGAAGGACCTGCTCCGCCGCGACGTGTTGTACTACAAGGGCCGGCTG
  GACATGGACGGCCTGGAGGTGGTGGACCTGGAGGACGGGAAGGACAGAGACCTCCATGTGAGCATCAAGA
  ACGCCTTCCGGCTGCACCGTGGCGCCACAGGGGACAGCCACCTGCTGTGCACCAGGAAGCCCGAGCAGAA
  GCAGCGCTGGCTCAAGGCCTTTGCCAGGGAGAGGGAGCAGGTGCAGCTGGACCAGGAGACAGGCTTCTCC
  ATCACTGAACTGCAGAGGAAGCAGGCCATGCTGAATGCCAGCAAGCAGCAGGTCACAGGGAAGCCCAAAG
  CTGTTGGCCGGCCCTGCTACCTGACGCGCCAGAAGCACCCAGCCCTGCCCAGCAACCGGCCCCAGCAGCA
  GGTCCTGGTGCTGGCGGAGCCCAGGCGCAAGCCATCTACCTTCTGGCACAGCATCAGCCGGCTGGCACCC
  TTCCGCAAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG215591 representing NM_015320
  Red=Cloning site Green=Tags(s)
  
  MGLHYPGRGSAISMVSLGSYSYVDSSSGDPERPKIPKGQTSFLLSLQTLNQDEQKEESREGGQGPRGLGT
  VPWLRDLPGSENHMPWEEPAGEKPSCSHSQKAFHMEPAQKPCFTTDMVTWALLCISAETVRGEAPSQPRG
  IPHRSPVSVDDLWLEKTQRKKLQKQAHIERRLHIGAVHKDGVKCWRKTIITSPESLNLPRRSHPLSQSAP
  TGLNHMGWPEHTPGTAMPDGALDTAVCADEVGSEEDLYDDLHSSSHHYSHPGGGGEQLAINELISDGSVV
  CAEALWDHVTMDDQELGFKAGDVIEVMDATNREWWWGRVADGEGWFPASFVRLRVNQDEPADDDAPLAGN
  SGAEDGGAEAQSSKDQMRTNVINEILSTERDYIKHLRDICEGYVRQCRKRADMFSEEQLRTIFGNIEDIY
  RCQKAFVKALEQRFNRERPHLSELGACFLEHQADFQIYSEYCNNHPNACVELSRLTKLSKYVYFFEACRL
  LQKMIDISLDGFLLTPVQKICKYPLQLAELLKYTHPQHRDFKDVEAALHAMKNVAQLINERKRRLENIDK
  IAQWQSSIEDWEGEDLLVRSSELIYSGELTRVTQPQAKSQQRMFFLFDHQLIYCKKDLLRRDVLYYKGRL
  DMDGLEVVDLEDGKDRDLHVSIKNAFRLHRGATGDSHLLCTRKPEQKQRWLKAFAREREQVQLDQETGFS
  ITELQRKQAMLNASKQQVTGKPKAVGRPCYLTRQKHPALPSNRPQQQVLVLAEPRRKPSTFWHSISRLAP
  FRK
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015320
• ORF Size: 2319 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_015320.2, NP_056135.2
• RefSeq Size: 3667
• RefSeq ORF: 2073
• Locus ID: 50649
• Protein Families: Druggable Genome
• Protein Pathways: Regulation of actin cytoskeleton
• Gene Summary: Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The protein encoded by this gene may form complex with G proteins and stimulate Rho-dependent signals. Multiple alternatively spliced transcript variants encoding different isoforms have been found, but the full-length nature of some variants has not been determined. [provided by RefSeq, Jun 2013]