BTF (BCLAF1) (NM_001077441) Human Tagged ORF Clone

CAT#: RG215897

BCLAF1 (GFP-tagged) - Human BCL2-associated transcription factor 1 (BCLAF1), transcript variant 3

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: BCLAF1
• Synonyms: bK211L9.1; BTF
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG215897 representing NM_001077441
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGTCGCTCCAATTCTAGATCACATTCTTCAAGGTCAAAGTCTAGATCACAGTCTAGTTCTCGATCAA
  GATCAAGATCTCATTCTAGAAAGAAGCGATACAGTTCTAGGTCTCGTTCCAGAACATATTCAAGGTCTCG
  TAGTAGAGATCGTATGTATTCTAGAGATTATCGTCGCGATTACAGAAATAATAGAGGAATGAGACGACCT
  TATGGGTACAGAGGAAGGGGTAGAGGGTATTATCAAGGAGGAGGAGGTAGATATCATCGAGGTGGTTATA
  GACCTGTCTGGAATAGAAGGCACTCTAGGAGTCCTAGACGAGGTCGTTCACGTTCCAGGAGTCCAAAAAG
  AAGATCCGTTTCTTCTCAAAGATCCAGAAGCAGATCTCGCCGGTCATATAGATCTTCTAGGTCTCCAAGA
  TCATCCTCTTCTCGTTCTTCATCCCCATATAGCAAATCTCCTGTTTCTAAAAGACGAGGGTCTCAGGAAA
  AACAAACCAAAAAAGCTGAAGGGGAACCCCAAGAAGAGAGTCCGTTGAAAAGTAAATCACAGGAGGAACC
  GAAAGATACATTTGAACATGACCCATCTGAGTCTATCGATGAATTTAATAAGTCATCAGCCACATCCGGT
  GATATTTGGCCTGGCCTTTCAGCTTATGATAATAGTCCTAGATCACCCCATAGTCCTTCACCTATTGCTA
  CACCACCTAGTCAGAGTTCATCTTGCTCTGATGCTCCCATGCTCAGTACAGTTCACTCTGCAAAAAATAC
  TCCTTCTCAGCATTCACATTCCATTCAGCATAGTCCTGAAAGGTCTGGGTCTGGTTCTGTTGGAAATGGA
  TCTAGTCGATACAGTCCTTCTCAGAATAGTCCAATTCATCACATCCCTTCACGAAGAAGTCCTGCAAAGA
  CAATCGCACCACAGAATGCTCCAAGAGATGAGTCTAGGGGCCGTTCCTCGTTTTATCCTGATGGTGGAGA
  TCAGGAAACTGCAAAGACTGGGAAGTTCTTAAAAAGTCCCCCTCTACACAAGAATCTGGATGCACGAGAA
  AAGTCTACCTTCAGAGAGGAAAGCCCACTTAGGATCAAAATGATAGCGAGTGATTCTCACCGTCCTGAAG
  TCAAACTCAAAATGGCACCTGTTCCTCTTGATGATTCTAACAGACCTGCTTCCTTGACTAAAGACAGGCT
  GCTTGCTAGTACACTTGTCCATTCTGTCAAGAAGGAGCAAGAATTCCGATCCATCTTTGACCACATTAAG
  TTGCCACAGGCCAGCAAAAGCACTTCAGAGTCATTTATTCAACACATTGTGTCCTTGGTTCATCATGTTA
  AAGAGCAATACTTCAAGTCAGCTGCAATGACCCTAAACGAGCGGTTCACTTCGTATCAGAAAGCCACTGA
  AGAACATAGTACTCGGCAAAAGAGCCCTGAAATACACAGGAGAATTGACATCTCACCAAGTACCCTGAGG
  AAGCATACCCGTTTAGCAGGGGAAGAGAGAGTTTTTAAAGAAGAAAATCAAAAGGGAGATAAAAAATTAA
  GGTGTGACTCTGCTGACCTTCGGCATGACATTGATCGCCGTAGAAAAGAAAGAAGTAAAGAACGGGGAGA
  TTCCAAGGGCTCCAGGGAATCCAGTGGATCAAGAAAGCAGGAAAAAACTCCAAAAGATTACAAGGAATAC
  AAATCTTACAAAGATGACAGTAAACATAAAAGAGAGCAAGATCATTCTCGATCTTCATCCTCTTCAGCAT
  CACCTTCTTCTCCCAGTTCTCGAGAAGAAAAGGAGAGTAAGAAGGAAAGAGAAGAAGAATTTAAAACTCA
  CCATGAAATGAAAGAATACTCAGGCTTTGCAGGAGTTAGCCGACCACGAGGAACCTTTTTTCGAATTAGA
  GGCAGAGGAAGAGCCAGAGGAGTTTTTGCTGGGACAAATACTGGTCCAAACAACTCAAATACTACTTTTC
  AAAAGAGACCGAAGGAAGAGGAATGGGATCCAGAATATACCCCAAAGAGCAAGAAGTACTTCTTGCATGA
  CGACAGAGATGATGGTGTGGATTATTGGGCCAAAAGAGGAAGAGGTCGTGGTACTTTTCAACGTGGCAGA
  GGGCGCTTTAACTTCAAAAAATCAGGTAGCAGTCCTAAATGGACTCATGACAAATACCAAGGGGATGGGA
  TTGTTGAAGATGAAGAAGAGACCATGGAAAATAATGAAGAAAAGAAGGACAGACGCAAGGAAGAAAAGGA
  A
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG215897 representing NM_001077441
  Red=Cloning site Green=Tags(s)
  
  MGRSNSRSHSSRSKSRSQSSSRSRSRSHSRKKRYSSRSRSRTYSRSRSRDRMYSRDYRRDYRNNRGMRRP
  YGYRGRGRGYYQGGGGRYHRGGYRPVWNRRHSRSPRRGRSRSRSPKRRSVSSQRSRSRSRRSYRSSRSPR
  SSSSRSSSPYSKSPVSKRRGSQEKQTKKAEGEPQEESPLKSKSQEEPKDTFEHDPSESIDEFNKSSATSG
  DIWPGLSAYDNSPRSPHSPSPIATPPSQSSSCSDAPMLSTVHSAKNTPSQHSHSIQHSPERSGSGSVGNG
  SSRYSPSQNSPIHHIPSRRSPAKTIAPQNAPRDESRGRSSFYPDGGDQETAKTGKFLKSPPLHKNLDARE
  KSTFREESPLRIKMIASDSHRPEVKLKMAPVPLDDSNRPASLTKDRLLASTLVHSVKKEQEFRSIFDHIK
  LPQASKSTSESFIQHIVSLVHHVKEQYFKSAAMTLNERFTSYQKATEEHSTRQKSPEIHRRIDISPSTLR
  KHTRLAGEERVFKEENQKGDKKLRCDSADLRHDIDRRRKERSKERGDSKGSRESSGSRKQEKTPKDYKEY
  KSYKDDSKHKREQDHSRSSSSSASPSSPSSREEKESKKEREEEFKTHHEMKEYSGFAGVSRPRGTFFRIR
  GRGRARGVFAGTNTGPNNSNTTFQKRPKEEEWDPEYTPKSKKYFLHDDRDDGVDYWAKRGRGRGTFQRGR
  GRFNFKKSGSSPKWTHDKYQGDGIVEDEEETMENNEEKKDRRKEEKE
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001077441
• ORF Size: 2241 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001077441.1, NP_001070909.1
• RefSeq Size: 6758
• RefSeq ORF: 2244
• Locus ID: 9774
• Protein Families: Transcription Factors
• Gene Summary: This gene encodes a transcriptional repressor that interacts with several members of the BCL2 family of proteins. Overexpression of this protein induces apoptosis, which can be suppressed by co-expression of BCL2 proteins. The protein localizes to dot-like structures throughout the nucleus, and redistributes to a zone near the nuclear envelope in cells undergoing apoptosis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]