HIST1H2BA (NM_170610) Human Tagged ORF Clone

CAT#: RG215946

HIST1H2BA (GFP-tagged) - Human histone cluster 1, H2ba (HIST1H2BA)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: HIST1H2BA
• Synonyms: bA317E16.3; H2BFU; hTSH2B; STBP; TH2B; TSH2B; TSH2B.1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG215946 representing NM_170610
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGGAGGTGTCATCTAAAGGTGCTACCATTTCCAAGAAGGGCTTTAAGAAAGCTGTCGTTAAGACCC
  AGAAAAAGGAAGGCAAAAAGCGCAAGAGGACCCGTAAGGAGAGTTATTCTATTTACATTTACAAAGTGCT
  AAAGCAGGTCCATCCGGACACTGGCATCTCTTCGAAAGCTATGAGCATTATGAATTCTTTCGTCACTGAT
  ATCTTTGAGCGTATAGCGAGCGAGGCATCACGTTTGGCTCACTACAGCAAGCGCTCCACCATTTCTTCCA
  GAGAGATTCAGACAGCAGTGCGCTTGCTACTGCCGGGAGAGCTGGCTAAACATGCTGTGTCTGAGGGCAC
  CAAGGCTGTCACTAAGTACACCAGCTCCAAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG215946 representing NM_170610
  Red=Cloning site Green=Tags(s)
  
  MPEVSSKGATISKKGFKKAVVKTQKKEGKKRKRTRKESYSIYIYKVLKQVHPDTGISSKAMSIMNSFVTD
  IFERIASEASRLAHYSKRSTISSREIQTAVRLLLPGELAKHAVSEGTKAVTKYTSSK
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_170610
• ORF Size: 381 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_170610.2, NP_733759.1
• RefSeq Size: 437
• RefSeq ORF: 384
• Locus ID: 255626
• Protein Pathways: Systemic lupus erythematosus
• Gene Summary: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a testis/sperm-specific member of the histone H2B family. Transcripts from this gene contain a palindromic termination element. [provided by RefSeq, Aug 2015]