SPAG6 (NM_012443) Human Tagged ORF Clone

CAT#: RG216144

SPAG6 (GFP-tagged) - Human sperm associated antigen 6 (SPAG6), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: SPAG6
• Synonyms: CT141; pf16; Repro-SA-1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG216144 representing NM_012443
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTCAGAGGCAGGTGCTGCAAGTGTTCGAGCAATACCAGAAGGCCAGGACCCAGTTCGTGCAGATGG
  TGGCGGAGCTGGCGACTAGACCCCAAAACATCGAGACGCTGCAGAACGCGGGTGTAATGTCTTTGCTGAG
  AACTCTTCTTCTGGACGTGGTCCCAACAATTCAACAGACTGCTGCTTTGGCTCTTGGGAGACTGGCCAAT
  TATAATGATGACCTAGCAGAAGCTGTTGTGAAGTGCGACATTCTTCCACAGCTTGTTTATTCATTGGCAG
  AACAGAATCGCTTCTACAAGAAAGCAGCTGCCTTTGTGTTACGAGCAGTTGGTAAACATTCTCCCCAGCT
  AGCTCAGGCAATAGTCGATTGTGGAGCACTGGATACGCTGGTCATATGCTTGGAAGATTTTGACCCTGGA
  GTCAAGGAGGCTGCAGCCTGGGCACTTAGATATATTGCAAGACATAATGCAGAACTGTCACAAGCTGTGG
  TGGATGCAGGAGCTGTTCCTCTTTTAGTACTCTGTATCCAGGAGCCAGAAATTGCTTTGAAAAGGATTGC
  TGCTTCGGCCCTCAGTGATATTGCAAAGCATTCTCCAGAGTTAGCACAGACAGTAGTGGATGCAGGAGCT
  GTTGCTCATTTAGCCCAGATGATCCTGAACCCTGATGCTAAATTGAAGCATCAGATCCTTTCAGCTCTCA
  GTCAGGTTTCAAAACATTCCGTGGATCTGGCAGAAATGGTTGTTGAAGCAGAGATTTTTCCAGTTGTACT
  TACCTGTCTGAAGGACAAGGATGAATACGTGAAGAAAAATGCTTCTACTTTAATTAGAGAGATTGCAAAA
  CATACACCCGAGCTTTCACAGCTGGTAGTTAACGCAGGAGGGGTTGCTGCCGTGATTGACTGCATTGGGT
  CCTGCAAAGGGAACACACGGCTGCCTGGCATCATGATGCTTGGTTATGTAGCAGCTCATTCTGAGAACCT
  AGCAATGGCAGTCATCATTTCTAAGGGTGTACCCCAGTTGTCAGTCTGCTTGTCAGAAGAACCGGAAGAT
  CATATTAAGGCTGCAGCTGCTTGGGCCTTAGGACAGATTGGAAGACACACTCCTGAACACGCACGGGCTG
  TTGCAGTCACAAATACTTTGCCAGTTCTGCTTTCTTTGTACATGTCAACAGAAAGTTCTGAGGATCTCCA
  AGTAAAAAGTAAAAAAGCCATAAAGAATATCCTGCAAAAATGTACCTACTTACCAGCCCTTGAACCATTT
  CTATATGATGCTCCTCCCAATATTCTGAAACATGTGGTTGGACAGTTCAGTAAGGTGCTGCCGCATGATA
  GCAAAGCTCGACGACTTTTTGTAACAAGTGGTGGCCTTAAAAAAGTTCAAGAGATAAAAGCAGAACCTGG
  TTCTCTCCTTCAAGAATACATCAACAGTATTAACAGTTGTTACCCCGAGGAAATAGTGAGGTATTATTCC
  CCTGGATATTCAGATACACTTCTGCAGAGGGTGGACAGCTATCAACCACTTAATAAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG216144 representing NM_012443
  Red=Cloning site Green=Tags(s)
  
  MSQRQVLQVFEQYQKARTQFVQMVAELATRPQNIETLQNAGVMSLLRTLLLDVVPTIQQTAALALGRLAN
  YNDDLAEAVVKCDILPQLVYSLAEQNRFYKKAAAFVLRAVGKHSPQLAQAIVDCGALDTLVICLEDFDPG
  VKEAAAWALRYIARHNAELSQAVVDAGAVPLLVLCIQEPEIALKRIAASALSDIAKHSPELAQTVVDAGA
  VAHLAQMILNPDAKLKHQILSALSQVSKHSVDLAEMVVEAEIFPVVLTCLKDKDEYVKKNASTLIREIAK
  HTPELSQLVVNAGGVAAVIDCIGSCKGNTRLPGIMMLGYVAAHSENLAMAVIISKGVPQLSVCLSEEPED
  HIKAAAAWALGQIGRHTPEHARAVAVTNTLPVLLSLYMSTESSEDLQVKSKKAIKNILQKCTYLPALEPF
  LYDAPPNILKHVVGQFSKVLPHDSKARRLFVTSGGLKKVQEIKAEPGSLLQEYINSINSCYPEEIVRYYS
  PGYSDTLLQRVDSYQPLNN
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012443
• ORF Size: 1527 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_012443.2, NP_036575.1
• RefSeq Size: 2630
• RefSeq ORF: 1530
• Locus ID: 9576
• Gene Summary: The correlation of anti-sperm antibodies with cases of unexplained infertility implicates a role for these antibodies in blocking fertilization. Improved diagnosis and treatment of immunologic infertility, as well as identification of proteins for targeted contraception, are dependent on the identification and characterization of relevant sperm antigens. The protein expressed by this gene is recognized by anti-sperm antibodies from an infertile man. This protein localizes to the tail of permeabilized human sperm and contains eight contiguous armadillo repeats, a motif known to mediate protein-protein interactions. Studies in mice suggest that this protein is involved in sperm flagellar motility and maintenance of the structural integrity of mature sperm. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]