Phospholipase C beta 1 (PLCB1) (NM_182734) Human Tagged ORF Clone

CAT#: RG216150

PLCB1 (GFP-tagged) - Human phospholipase C, beta 1 (phosphoinositide-specific) (PLCB1), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PLCB1
• Synonyms: EIEE12; PI-PLC; PLC-154; PLC-beta-1; PLC-I; PLC154; PLCB1A; PLCB1B
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG216150 representing NM_182734
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGGGGCTCAACCCGGAGTGCACGCCTTGCAACTCAAGCCCGTGTGCGTGTCCGACAGCCTCAAGA
  AGGGCACCAAATTCGTCAAGTGGGATGATGATTCAACTATTGTTACTCCAATTATTTTGAGGACTGACCC
  TCAGGGATTTTTCTTTTACTGGACAGATCAAAACAAGGAGACAGAGCTACTGGATCTCAGCCTTGTCAAA
  GATGCCAGATGTGGGAGACACGCCAAAGCTCCCAAGGACCCCAAATTACGTGAACTTTTGGATGTGGGGA
  ACATCGGGCGCCTGGAGCAGCGCATGATCACAGTGGTGTATGGGCCTGACCTCGTGAACATCTCCCATTT
  GAATCTCGTGGCTTTCCAAGAAGAAGTGGCCAAGGAATGGACAAATGAGGTTTTCAGTTTGGCAACAAAC
  CTGCTGGCCCAAAACATGTCCAGGGATGCATTTCTGGAAAAAGCCTATACTAAACTTAAGCTGCAAGTCA
  CTCCAGAAGGGCGTATTCCTCTCAAAAACATATATCGCTTGTTTTCAGCAGATCGGAAGCGAGTTGAAAC
  TGCTTTAGAGGCTTGTAGTCTTCCATCTTCAAGGAATGATTCAATACCTCAAGAAGATTTCACTCCAGAA
  GTGTACAGAGTTTTCCTCAACAACCTTTGCCCTCGACCTGAAATTGATAACATCTTTTCAGAATTTGGTG
  CAAAAAGCAAACCATATCTTACCGTTGATCAGATGATGGATTTTATCAACCTTAAGCAGCGAGATCCTCG
  GCTTAATGAAATACTTTATCCACCTCTAAAACAAGAGCAAGTCCAAGTATTGATTGAGAAGTATGAACCC
  AACAACAGCCTCGCCAGAAAAGGACAAATATCAGTGGATGGGTTCATGCGCTATCTGAGTGGAGAAGAAA
  ACGGAGTCGTTTCACCTGAGAAACTGGATTTGAATGAAGACATGTCTCAGCCCCTTTCTCACTATTTCAT
  TAATTCCTCGCACAACACCTACCTCACAGCTGGCCAACTGGCTGGAAACTCCTCTGTTGAGATGTATCGC
  CAAGTGCTCCTGTCTGGTTGTCGCTGTGTGGAGCTGGACTGCTGGAAGGGACGGACTGCAGAAGAGGAAC
  CTGTCATCACCCATGGCTTCACCATGACAACTGAAATATCTTTCAAGGAAGTGATAGAAGCAATTGCGGA
  GTGTGCATTTAAGACTTCACCTTTTCCAATTCTCCTTTCGTTTGAGAACCATGTGGATTCCCCAAAGCAG
  CAAGCCAAGATGGCGGAGTACTGCCGACTGATCTTTGGGGATGCCCTTCTCATGGAGCCCCTGGAAAAAT
  ATCCACTGGAATCTGGAGTTCCTCTTCCAAGCCCTATGGATTTAATGTATAAAATTTTGGTGAAAAATAA
  GAAGAAATCACACAAGTCATCAGAAGGAAGCGGCAAAAAGAAGCTCTCAGAACAAGCCTCCAACACCTAC
  AGTGACTCCTCCAGCATGTTCGAGCCCTCATCCCCAGGAGCCGGAGAAGCTGATACGGAAAGTGACGACG
  ACGATGATGATGATGACTGTAAAAAATCTTCAATGGATGAGGGGACTGCTGGAAGTGAGGCTATGGCCAC
  AGAAGAAATGTCTAATCTGGTGAACTATATTCAGCCAGTCAAGTTTGAGTCATTTGAAATTTCAAAAAAA
  AGAAATAAAAGTTTTGAAATGTCTTCCTTCGTGGAAACCAAAGGACTTGAACAACTCACCAAGTCTCCAG
  TGGAATTTGTAGAATATAACAAAATGCAGCTTAGCAGGATATATCCAAAAGGAACACGTGTGGATTCATC
  CAACTATATGCCTCAGCTCTTCTGGAATGCAGGTTGTCAGATGGTGGCACTTAATTTCCAGACAATGGAC
  CTGGCTATGCAAATAAATATGGGGATGTATGAATACAACGGGAAGAGTGGCTACAGATTGAAGCCAGAGT
  TCATGAGGAGGCCTGACAAGCATTTTGATCCATTTACTGAAGGCATCGTAGATGGGATAGTGGCAAACAC
  TTTGTCTGTTAAGATTATTTCAGGTCAGTTTCTTTCTGATAAGAAAGTTGGGACTTACGTGGAAGTAGAT
  ATGTTTGGTTTGCCTGTGGATACAAGGAGGAAGGCATTTAAGACCAAAACATCCCAGGGAAATGCTGTGA
  ATCCTGTCTGGGAAGAAGAACCTATTGTGTTCAAAAAGGTGGTTCTTCCTACTCTGGCCTGTTTGAGAAT
  AGCAGTTTATGAAGAAGGAGGTAAATTCATTGGCCACCGTATCTTGCCAGTGCAAGCCATTCGGCCAGGC
  TATCACTATATCTGTCTAAGGAATGAAAGGAACCAGCCTCTGACGCTGCCTGCTGTCTTTGTCTACATAG
  AAGTGAAAGACTATGTGCCAGACACATATGCAGATGTCATCGAAGCTTTATCAAACCCAATCCGATATGT
  GAACCTGATGGAACAGAGAGCTAAGCAATTGGCTGCTTTGACACTGGAAGATGAAGAAGAAGTAAAGAAA
  GAGGCTGATCCTGGAGAAACACCATCAGAGGCTCCAAGTGAAGCGAGAACGACTCCAGCAGAAAATGGGG
  TGAATCACACTACAACCCTGACACCCAAGCCACCCTCCCAGGCTCTCCACAGCCAGCCAGCTCCAGGTTC
  TGTAAAGGCACCTGCCAAAACAGAAGATCTTATTCAGAGTGTCTTAACAGAAGTGGAAGCACAGACCATC
  GAAGAACTAAAGCAACAGAAATCGTTTGTGAAACTTCAAAAGAAACACTACAAAGAAATGAAAGACCTGG
  TTAAGAGACACCACAAGAAAACCACTGACCTTATCAAAGAACACACTACCAAGTATAATGAAATTCAGAA
  TGACTACTTGAGAAGGAGAGCCGCTTTGGAAAAGTCCGCCAAAAAGGACAGTAAGAAAAAATCGGAACCC
  AGCAGCCCTGATCATGGTTCATCAACGATTGAGCAAGACCTCGCTGCCCTGGATGCTGAAATGACCCAAA
  AGTTAATAGACTTGAAGGACAAACAACAGCAGCAGCTGCTTAATCTTCGGCAAGAACAGTATTATAGTGA
  AAAATACCAGAAGCGAGAACATATTAAACTGCTTATTCAAAAGTTGACGGATGTCGCAGAAGAGTGTCAG
  AACAATCAGTTAAAGAAGCTCAAAGAAATCTGTGAGAAAGAAAAGAAAGAATTAAAGAAGAAAATGGATA
  AAAAGAGGCAGGAGAAGATAACAGAAGCTAAATCCAAAGACAAAAGTCAGATGGAAGAGGAGAAGACAGA
  GATGATCCGGTTATATATCCAGGAAGTGGTGCAGTATATCAAGAGGCTAGAAGAAGCGCAAAGTAAACGG
  CAAGAAAAACTCGTAGAGAAACACAAGGAAATACGTCAGCAGATCCTGGATGAAAAGCCCAAGCTGCAGG
  TGGAGCTGGAGCAAGAATACCAAGACAAATTCAAAAGACTGCCCCTCGAGATTTTGGAATTCGTGCAGGA
  AGCCATGAAAGGAAAGATCAGTGAAGACAGCAATCACGGTTCTGCCCCTCTCTCCCTGTCCTCAGACCCT
  GGAAAAGTGAACCACAAGACTCCCTCCAGTGAGGAGCTGGGAGGAGACATCCCAGGAAAAGAATTTGATA
  CTCCTCTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG216150 representing NM_182734
  Red=Cloning site Green=Tags(s)
  
  MAGAQPGVHALQLKPVCVSDSLKKGTKFVKWDDDSTIVTPIILRTDPQGFFFYWTDQNKETELLDLSLVK
  DARCGRHAKAPKDPKLRELLDVGNIGRLEQRMITVVYGPDLVNISHLNLVAFQEEVAKEWTNEVFSLATN
  LLAQNMSRDAFLEKAYTKLKLQVTPEGRIPLKNIYRLFSADRKRVETALEACSLPSSRNDSIPQEDFTPE
  VYRVFLNNLCPRPEIDNIFSEFGAKSKPYLTVDQMMDFINLKQRDPRLNEILYPPLKQEQVQVLIEKYEP
  NNSLARKGQISVDGFMRYLSGEENGVVSPEKLDLNEDMSQPLSHYFINSSHNTYLTAGQLAGNSSVEMYR
  QVLLSGCRCVELDCWKGRTAEEEPVITHGFTMTTEISFKEVIEAIAECAFKTSPFPILLSFENHVDSPKQ
  QAKMAEYCRLIFGDALLMEPLEKYPLESGVPLPSPMDLMYKILVKNKKKSHKSSEGSGKKKLSEQASNTY
  SDSSSMFEPSSPGAGEADTESDDDDDDDDCKKSSMDEGTAGSEAMATEEMSNLVNYIQPVKFESFEISKK
  RNKSFEMSSFVETKGLEQLTKSPVEFVEYNKMQLSRIYPKGTRVDSSNYMPQLFWNAGCQMVALNFQTMD
  LAMQINMGMYEYNGKSGYRLKPEFMRRPDKHFDPFTEGIVDGIVANTLSVKIISGQFLSDKKVGTYVEVD
  MFGLPVDTRRKAFKTKTSQGNAVNPVWEEEPIVFKKVVLPTLACLRIAVYEEGGKFIGHRILPVQAIRPG
  YHYICLRNERNQPLTLPAVFVYIEVKDYVPDTYADVIEALSNPIRYVNLMEQRAKQLAALTLEDEEEVKK
  EADPGETPSEAPSEARTTPAENGVNHTTTLTPKPPSQALHSQPAPGSVKAPAKTEDLIQSVLTEVEAQTI
  EELKQQKSFVKLQKKHYKEMKDLVKRHHKKTTDLIKEHTTKYNEIQNDYLRRRAALEKSAKKDSKKKSEP
  SSPDHGSSTIEQDLAALDAEMTQKLIDLKDKQQQQLLNLRQEQYYSEKYQKREHIKLLIQKLTDVAEECQ
  NNQLKKLKEICEKEKKELKKKMDKKRQEKITEAKSKDKSQMEEEKTEMIRLYIQEVVQYIKRLEEAQSKR
  QEKLVEKHKEIRQQILDEKPKLQVELEQEYQDKFKRLPLEILEFVQEAMKGKISEDSNHGSAPLSLSSDP
  GKVNHKTPSSEELGGDIPGKEFDTPL
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_182734
• ORF Size: 3519 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_182734.1, NP_877398.1
• RefSeq Size: 6823
• RefSeq ORF: 3522
• Locus ID: 23236
• Protein Families: Druggable Genome
• Protein Pathways: Alzheimer's disease, Calcium signaling pathway, Chemokine signaling pathway, Gap junction, GnRH signaling pathway, Huntington's disease, Inositol phosphate metabolism, Long-term depression, Long-term potentiation, Melanogenesis, Metabolic pathways, Phosphatidylinositol signaling system, Vascular smooth muscle contraction, Wnt signaling pathway
• Gene Summary: The protein encoded by this gene catalyzes the formation of inositol 1,4,5-trisphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. This reaction uses calcium as a cofactor and plays an important role in the intracellular transduction of many extracellular signals. This gene is activated by two G-protein alpha subunits, alpha-q and alpha-11. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]