CREM (NM_183060) Human Tagged ORF Clone
CAT#: RG216170
- TrueORF®
CREM (GFP-tagged) - Human cAMP responsive element modulator (CREM), transcript variant 22
"NM_183060" in other vectors (4)
Product Images
![](https://cdn.origene.com/img/defaults-img-expression-plasmids.jpg)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Symbol | CREM |
Synonyms | CREM-2; hCREM-2; ICER |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG216170 representing NM_183060
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACCATGGAAACAGTTGAATCCCAGCATGATGGAAGTATAACAGCTTCTTTGACAGAGAGCAAGTCTG CTCATGTGCAGACTCAGACTGGCCAAAATTCAATCCCTGCTTTAGCTCAGGTAGCAGCAATTGCAGAGAC AGATGAATCTGCAGAATCAGAAGGTGTAATTGATTCTCATAAACGTAGAGAAATCCTTTCACGAAGACCC TCTTATAGGAAAATACTGAATGAACTGTCCTCTGATGTGCCTGGTGTTCCCAAGATTGAAGAAGAGAGAT CAGAGGAAGAAGGAACACCACCTAGTATTGCTACCATGGCAGTACCAACTAGCATATATCAGACTAGCAC GGGGCAATACACTGCCACTGGTGACATGCCAACTTACCAGATCCGAGCTCCTACTGCTGCTTTGCCACAG GGAGTGGTGATGGCTGCATCGCCCGGAAGTTTGCACAGTCCCCAGCAGCTGGCAGAAGAAGCAACACGCA AACGAGAGCTGAGGCTAATGAAAAACAGGGAAGCTGCCAAAGAATGTCGACGTCGAAAGAAAGAATATGT AAAATGTCTGGAGAGCCGAGTTGCAGTGCTGGAAGTCCAGAACAAGAAGCTTATAGAGGAACTTGAAACC TTGAAAGACATTTGTTCTCCCAAAACAGATTAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG216170 representing NM_183060
Red=Cloning site Green=Tags(s) MTMETVESQHDGSITASLTESKSAHVQTQTGQNSIPALAQVAAIAETDESAESEGVIDSHKRREILSRRP SYRKILNELSSDVPGVPKIEEERSEEEGTPPSIATMAVPTSIYQTSTGQYTATGDMPTYQIRAPTAALPQ GVVMAASPGSLHSPQQLAEEATRKRELRLMKNREAAKECRRRKKEYVKCLESRVAVLEVQNKKLIEELET LKDICSPKTDY TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_183060 |
ORF Size | 663 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_183060.2, NP_898883.1 |
RefSeq Size | 1941 bp |
RefSeq ORF | 666 bp |
Locus ID | 1390 |
Cytogenetics | 10p11.21 |
Protein Families | Druggable Genome, Transcription Factors |
Gene Summary | 'This gene encodes a bZIP transcription factor that binds to the cAMP responsive element found in many viral and cellular promoters. It is an important component of cAMP-mediated signal transduction during the spermatogenetic cycle, as well as other complex processes. Alternative promoter and translation initiation site usage allows this gene to exert spatial and temporal specificity to cAMP responsiveness. Multiple alternatively spliced transcript variants encoding several different isoforms have been found for this gene, with some of them functioning as activators and some as repressors of transcription. [provided by RefSeq, Jul 2008]' |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC307554 | CREM (untagged)-Human cAMP responsive element modulator (CREM), transcript variant 22 |
USD 420.00 |
|
RC216170 | CREM (Myc-DDK-tagged)-Human cAMP responsive element modulator (CREM), transcript variant 22 |
USD 420.00 |
|
RC216170L3 | Lenti-ORF clone of CREM (Myc-DDK-tagged)-Human cAMP responsive element modulator (CREM), transcript variant 22 |
USD 620.00 |
|
RC216170L4 | Lenti-ORF clone of CREM (mGFP-tagged)-Human cAMP responsive element modulator (CREM), transcript variant 22 |
USD 620.00 |
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