AMELX (NM_001142) Human Tagged ORF Clone
CAT#: RG216306
- TrueORF®
AMELX (GFP-tagged) - Human amelogenin, X-linked (AMELX), transcript variant 1
"NM_001142" in other vectors (4)
Product Images
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Symbol | AMELX |
Synonyms | AI1E; AIH1; ALGN; AMG; AMGL; AMGX |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG216306 representing NM_001142
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGACCTGGATTTTATTTGCCTGCCTCCTGGGAGCAGCTTTTGCCATGCCTCTACCACCTCATCCTG GGCACCCTGGTTATATCAACTTCAGCTATGAGGTGCTTACCCCTTTGAAGTGGTACCAGAGCATAAGGCC ACCGTACCCTTCCTATGGTTACGAGCCCATGGGTGGATGGCTGCACCACCAAATCATCCCCGTGCTGTCC CAACAGCACCCCCCGACTCACACCCTGCAGCCTCATCACCACATCCCAGTGGTGCCAGCTCAGCAGCCCG TGATCCCCCAGCAACCAATGATGCCCGTTCCTGGCCAACACTCCATGACTCCAATCCAACACCACCAGCC AAACCTCCCTCCGCCCGCCCAGCAGCCCTACCAGCCCCAGCCTGTTCAGCCACAGCCTCACCAGCCCATG CAGCCCCAGCCACCTGTGCACCCCATGCAGCCCCTGCCGCCACAGCCACCTCTGCCTCCGATGTTCCCCA TGCAGCCCCTGCCTCCCATGCTTCCTGATCTGACTCTGGAAGCTTGGCCATCAACAGACAAGACCAAGCG GGAGGAAGTGGAT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG216306 representing NM_001142
Red=Cloning site Green=Tags(s) MGTWILFACLLGAAFAMPLPPHPGHPGYINFSYEVLTPLKWYQSIRPPYPSYGYEPMGGWLHHQIIPVLS QQHPPTHTLQPHHHIPVVPAQQPVIPQQPMMPVPGQHSMTPIQHHQPNLPPPAQQPYQPQPVQPQPHQPM QPQPPVHPMQPLPPQPPLPPMFPMQPLPPMLPDLTLEAWPSTDKTKREEVD TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_001142 |
ORF Size | 573 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001142.2, NP_001133.1 |
RefSeq Size | 793 bp |
RefSeq ORF | 576 bp |
Locus ID | 265 |
Cytogenetics | Xp22.2 |
Protein Families | Druggable Genome, Secreted Protein, Transmembrane |
Gene Summary | 'This gene encodes a member of the amelogenin family of extracellular matrix proteins. Amelogenins are involved in biomineralization during tooth enamel development. Mutations in this gene cause X-linked amelogenesis imperfecta. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]' |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC302979 | AMELX (untagged)-Human amelogenin, X-linked (AMELX), transcript variant 1 |
USD 420.00 |
|
RC216306 | AMELX (Myc-DDK-tagged)-Human amelogenin, X-linked (AMELX), transcript variant 1 |
USD 420.00 |
|
RC216306L3 | Lenti-ORF clone of AMELX (Myc-DDK-tagged)-Human amelogenin, X-linked (AMELX), transcript variant 1 |
USD 620.00 |
|
RC216306L4 | Lenti-ORF clone of AMELX (mGFP-tagged)-Human amelogenin, X-linked (AMELX), transcript variant 1 |
USD 620.00 |
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