FBXO43 (NM_001029860) Human Tagged ORF Clone

CAT#: RG216347

FBXO43 (GFP-tagged) - Human F-box protein 43 (FBXO43), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: FBXO43
• Synonyms: EMI2; ERP1; FBX43
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG216347 representing NM_001029860
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTTTTAAAGACAAAGATGAGAGAATTTCTTGTTTGGAAGCCTACGTAACTTTGACATCTAAGAGCT
  CAAGATTTACTGATGAAACAGAGATTTTGAAGATGTCGCAAAGGCACTCAGGTCAAGCTGGCACTGAAGC
  AGGAAATGGGGCGGACTCTCCTCCAATTGTCAACTCCAAGTACTCCACCTTCAGAGATTTTTGTTCCACA
  TCTTCATTTCAAGATAGTGGCTACAATGAGTTAAAATCTTGTAGCTTTGATAATATAGATAAAGAATATC
  TTGGAAAGAAAGAAAAAGGCCCAACATTACTCTATGAGCACCCTGAAACTTCAGGCCTGGGCTTAACACA
  TCCTTTAGAATCTCCCACTCAAAAAAAGAAATGTATCTTGCCTAGAAAGGAAAAGGATAAAACCCCAGAA
  CTTTGTGAAACACCTAAAATCAGTGGGAAAAAATGTTTACCTCGCAGAAGGTTGAATGTATCTTTCGCTC
  TTCTAAAAGGGGACTTTGAATCACAAAATAGTTCTTTAGAAAGTAGTATAAGCCAAGTTATCAACTTAGA
  AAAAAATATTCCAAGCAGTGCTTCAGGTTTTTCCAGGGCAAATAATTTTAGCCCTTTAGTTACTAGCACT
  TTAAAAACAGAAGAAGTGACTTCATGCAGTCAAAAATTGAGGCTTAATTTTTCTCAGCAAAAGACTTCCA
  CAATTGATGATTCCAAAGATGATTGTAGCCTATTTGAAGTTGAATGTATATCTCCAATTCAGGGCAATAA
  TTTTAAAGACTCTATCACACATGACTTTAGTGATAGCAGTTTATGCATTAATGATGAGAATGCATGTCCA
  GAGCTCCTGGGCTCCTCTGTTAGTGGAACAACTTGTGGAACAGATGAGGACATATTTGTGACTCCGATAA
  GTAATCTTGTGGCAAACATTAGATTTAACGCAAGTCAAATACTTTCTCCTTCACCTGAAGTGAGAGGCAG
  TATTTCAACGCCTGAAGACAGTGGTTTTAACTCACTTAGCTTGGAGAAATCAGAAGATTCCCTGTCTGAC
  CAGGAGGGTTCTTTTCAAGAACTACTGCAGAAACATAAGGGGACTCCCAAAGTTGGGGACACCATAAGAA
  AGACAAGACATCTTGGAAGGTCGAGAAGACTGTCCACCCTTCGGGAACAAAGCTCGCAGTCAGAGACAGA
  AGAGGAAAAGCAGATTGTCCACCCTGACTCTGAAAAAAGAGCAGCAGCTGCTTCTGCCATCTCAGAGGGT
  CAGCTGAGTAGTGATGAGAGTGGGGATTTGACCTTTAGCTTAAAGAATTTATCAAAGACCCCAGCCTTGC
  AATTGGTACATGAGCTGTTCATGAAAAGCAAGAGGAAAAGATTACAGGAAAATAGTGGACATGAATTCTT
  AGAGCAAGGGGATGGGGAGAAAATAGCTGTACTGCAGTGTATACTTGCAGGACTGATCGGCAAGAAAATG
  GGTATAGAAAAACTGGACATCTTAACAGAATTAAAATATAGAAATTTAAAGCATATTCTTGCTATGGTTT
  TAGAGTCCTTGACCGCAGAGAGCCTATGCAGTGTTTGGAAAGTAAGCAGAAATTGGCGTGAAATTGTTGT
  TCAAGATAAAAATGCAAATCGGAGGAGGAAATTTTATATCACACAACTGAAAACAGATTCTGAGGGGGCT
  GTATTAAATGTCGAGGATGCTGCCACTCGGCTCCAGCTTTTAAATCGCTCAGCTTTAAGATCTGTGCAGG
  CACAGGCTAGGATACCTGGTTCTCAGAGAGAGCAAGGGTCAACATTATCTCCCTGGGGAGAAGTTTTGAC
  ACCTCTAGCAAGCTCTTCTGTTACTCACTTAAGTAGTAAACAGGAAGAATATGTTAAGGTTGCCAAAACA
  CTTTTTACTGATGAAGCATTAAAACCTTGCCCAAGGTGCCAGTCCCCTGCTAAGTACCAGCCATATAAGA
  AAAGGGGACTGTGTAGCCGAACAGCCTGTGGTTTTGACTTTTGTGTGTTATGTCTGTGTGCTTATCATGG
  GTCTGAAGAATGTAGTAGAGGAGCAGCAAAGCCAAGAAATAGAAAAGATGCTCTCCCAGGAAGTGCCCAG
  AGTAAGCGGAATTTAAAACGCCTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG216347 representing NM_001029860
  Red=Cloning site Green=Tags(s)
  
  MSFKDKDERISCLEAYVTLTSKSSRFTDETEILKMSQRHSGQAGTEAGNGADSPPIVNSKYSTFRDFCST
  SSFQDSGYNELKSCSFDNIDKEYLGKKEKGPTLLYEHPETSGLGLTHPLESPTQKKKCILPRKEKDKTPE
  LCETPKISGKKCLPRRRLNVSFALLKGDFESQNSSLESSISQVINLEKNIPSSASGFSRANNFSPLVTST
  LKTEEVTSCSQKLRLNFSQQKTSTIDDSKDDCSLFEVECISPIQGNNFKDSITHDFSDSSLCINDENACP
  ELLGSSVSGTTCGTDEDIFVTPISNLVANIRFNASQILSPSPEVRGSISTPEDSGFNSLSLEKSEDSLSD
  QEGSFQELLQKHKGTPKVGDTIRKTRHLGRSRRLSTLREQSSQSETEEEKQIVHPDSEKRAAAASAISEG
  QLSSDESGDLTFSLKNLSKTPALQLVHELFMKSKRKRLQENSGHEFLEQGDGEKIAVLQCILAGLIGKKM
  GIEKLDILTELKYRNLKHILAMVLESLTAESLCSVWKVSRNWREIVVQDKNANRRRKFYITQLKTDSEGA
  VLNVEDAATRLQLLNRSALRSVQAQARIPGSQREQGSTLSPWGEVLTPLASSSVTHLSSKQEEYVKVAKT
  LFTDEALKPCPRCQSPAKYQPYKKRGLCSRTACGFDFCVLCLCAYHGSEECSRGAAKPRNRKDALPGSAQ
  SKRNLKRL
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001029860
• ORF Size: 2124 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001029860.2, NP_001025031.2
• RefSeq Size: 3211
• RefSeq ORF: 2127
• Locus ID: 286151
• Protein Pathways: Oocyte meiosis
• Gene Summary: Members of the F-box protein family, such as FBXO43, are characterized by an approximately 40-amino acid F-box motif. SCF complexes, formed by SKP1 (MIM 601434), cullin (see CUL1; MIM 603134), and F-box proteins, act as protein-ubiquitin ligases. F-box proteins interact with SKP1 through the F box, and they interact with ubiquitination targets through other protein interaction domains (Jin et al., 2004 [PubMed 15520277]). [supplied by OMIM, Mar 2008]