OBSL1 (NM_015311) Human Tagged ORF Clone

CAT#: RG217071

OBSL1 (GFP-tagged) - Human obscurin-like 1 (OBSL1), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: OBSL1
• Synonyms: KIAA0657; MGC71026
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG217071 representing NM_015311
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGGCGAGCTCGGGGGATCAGGGGAGCCCCCCGTGCTTCCTGCGCTTCCCGCGGCCTGTGCGGGTGG
  TAAGTGGCGCCGAGGCCGAGCTCAAGTGCGTGGTCCTGGGGGAGCCGCCGCCTGTAGTGGTGTGGGAGAA
  GGGCGGGCAGCAGCTGGCGGCCTCGGAACGCCTGAGCTTCCCGGCGGACGGCGCGGAGCACGGCCTGCTG
  CTGACCGCCGCACTGCCCACCGACGCGGGGGTCTACGTGTGCCGCGCCCGCAACGCGGCCGGCGAGGCCT
  ACGCGGCGGCCGCCGTCACCGTGCTGGAGCCGCCGGCCTCCGACCCCGAGCTGCAGCCCGCCGAGCGCCC
  GCTGCCATCGCCGGGGTCCGGGGAGGGCGCCCCGGTCTTCCTCACGGGGCCTCGATCCCAGTGGGTGCTG
  CGGGGGGCGGAGGTGGTGCTGACGTGCCGGGCGGGGGGCCTCCCCGAGCCCACACTGTACTGGGAGAAGG
  ACGGGATGGCCCTGGACGAAGTGTGGGACAGCAGCCACTTCGCGCTCCAGCCGGGCCGCGCCGAGGACGG
  CCCCGGCGCGAGCCTGGCACTGCGCATCCTGGCGGCTCGGCTGCCGGATTCCGGCGTCTACGTGTGCCAC
  GCCCGCAACGCGCACGGCCACGCGCAGGCGGGGGCGCTGCTCCAGGTGCACCAGCCCCCCGAGAGCCCGC
  CCGCGGACCCCGACGAGGCCCCCGCGCCGGTGGTGGAGCCGCTCAAGTGCGCGCCTAAGACCTTCTGGGT
  GAACGAGGGCAAGCACGCCAAGTTCCGCTGCTACGTGATGGGCAAGCCCGAGCCCGAGATCGAATGGCAC
  TGGGAGGGCCGCCCGCTGCTCCCGGACCGCCGCCGCCTCATGTACCGCGACCGCGACGGCGGCTTCGTGC
  TCAAGGTGCTTTACTGCCAGGCCAAGGATCGTGGGCTCTACGTCTGCGCCGCGCGCAACTCGGCGGGCCA
  GACGCTCAGTGCCGTGCAGCTGCACGTGAAAGAGCCCCGCCTCCGGTTCACACGGCCCCTGCAGGACGTG
  GAGGGCCGTGAGCACGGGATTGCCGTGCTGGAATGTAAAGTACCCAACTCCCGCATCCCCACGGCCTGGT
  TCCGTGAGGACCAGCGGCTGCTGCCCTGCCGCAAGTACGAGCAGATCGAAGAGGGCACTGTCCGGCGCCT
  CATCATCCACAGGCTGAAGGCAGACGATGATGGTATCTACCTGTGCGAGATGCGGGGCCGGGTGCGCACC
  GTGGCCAACGTCACAGTCAAAGGGCCCATCCTGAAGCGCCTGCCCCGGAAGCTCGACGTCCTGGAAGGAG
  AGAATGCTGTGCTGCTAGTGGAAACTCTAGAGGCCGGGGTCGAGGGACGCTGGAGCCGTGATGGGGAGGA
  GCTGCCGGTCATCTGCCAGAGCAGCTCAGGCCACATGCATGCCCTGGTCCTTCCAGGGGTCACCCGAGAG
  GATGCTGGCGAGGTCACCTTTAGCCTGGGCAACTCCCGTACCACTACGCTTCTCAGAGTAAAATGTGTCA
  AGCACAGTCCCCCAGGACCCCCCATATTGGCAGAGATGTTCAAGGGCCACAAGAACACGGTCCTGTTGAC
  CTGGAAGCCTCCCGAGCCAGCTCCCGAGACCCCATTCATCTACCGGCTGGAGCGGCAGGAAGTGGGCTCT
  GAAGACTGGATTCAGTGCTTCAGCATCGAGAAAGCCGGAGCCGTGGAGGTGCCGGGCGACTGTGTGCCCT
  CCGAGGGTGACTACCGCTTCCGCATCTGCACAGTCAGCGGACATGGCCGTAGTCCCCACGTGGTGTTCCA
  CGGTTCTGCTCACCTTGTGCCCACAGCTCGCCTGGTGGCAGGTCTGGAGGATGTGCAGGTATACGACGGG
  GAAGATGCCGTCTTCTCCCTCGATCTCTCCACCATCATCCAGGGTACCTGGTTCCTTAATGGGGAAGAGC
  TCAAGAGTAACGAGCCGGAGGGCCAGGTGGAACCTGGGGCCCTGCGGTACCGTATAGAGCAGAAGGGTCT
  GCAGCACAGACTCATCCTGCATGCCGTCAAGCACCAGGACAGCGGTGCCCTGGTCGGCTTCAGCTGCCCC
  GGCGTGCAGGACTCAGCTGCCCTCACAATCCAAGAGAGCCCGGTGCACATCCTGAGCCCCCAGGACAGGG
  TGTCGTTGACCTTCACAACCTCAGAGCGGGTGGTGCTGACTTGTGAGCTCTCAAGGGTGGACTTCCCGGC
  AACCTGGTACAAGGATGGGCAGAAGGTGGAGGAGAGCGAGTTGCTGGTGGTGAAGATGGATGGGCGCAAA
  CACCGTCTGATCCTGCCTGAGGCCAAAGTCCAGGACAGTGGCGAGTTTGAGTGCAGGACAGAAGGGGTCT
  CGGCCTTCTTCGGCGTCACTGTCCAAGATCCTCCCGTGCACATCGTGGACCCCCGAGAACATGTGTTCGT
  GCATGCCATAACTTCCGAGTGTGTCATGCTGGCCTGTGAGGTGGACCGAGAGGACGCCCCTGTGCGTTGG
  TACAAGGACGGGCAGGAGGTGGAGGAGAGTGACTTCGTGGTGCTGGAGAATGAGGGGCCCCATCGCCGCC
  TGGTGCTGCCCGCCACCCAGCCCTCAGACGGGGGCGAGTTTCAGTGCGTCGCTGGAGATGAGTGTGCCTA
  CTTCACTGTCACCATCACAGACGTCTCCTCGTGGATCGTGTATCCCAGCGGCAAGGTGTATGTGGCAGCC
  GTGCGCCTGGAGCGTGTGGTGCTGACCTGTGAGCTATGCCGGCCCTGGGCAGAGGTGCGCTGGACCAAGG
  ATGGAGAGGAGGTGGTGGAGAGCCCCGCGCTGCTCCTGCAGAAGGAAGACACTGTCCGCCGCCTGGTGCT
  GCCCGCTGTCCAGCTCGAGGACTCCGGCGAGTACTTGTGTGAAATTGACGATGAGTCGGCCTCCTTCACT
  GTCACCGTCACAGAACCCCCAGTGCGGATCATATACCCTCGCGATGAGGTGACCTTGATCGCCGTGACCT
  TGGAGTGTGTGGTGCTGATGTGTGAACTGTCTCGGGAGGATGCCCCTGTGCGCTGGTACAAGGATGGGCT
  GGAAGTGGAGGAGAGCGAGGCCCTGGTGCTGGAGAGGGATGGGCCACGCTGCCGCCTGGTGCTACCTGCT
  GCTCAGCCCGAGGACGGGGGCGAGTTTGTATGTGATGCTGGAGATGACTCGGCCTTCTTCACTGTCACTG
  TCACAGCCCCACCAGAGAGGATTGTGCACCCGGCAGCCCGCTCCCTGGATCTGCATTTTGGGGCTCCAGG
  GCGCGTGGAGCTGCGCTGTGAGGTGGCCCCAGCTGGGTCTCAGGTGCGCTGGTACAAGGACGGGCTGGAA
  GTGGAGGCATCAGATGCCCTGCAGCTGGGTGCCGAGGGGCCCACCCGCACCCTGACCCTGCCCCACGCCC
  AGCCTGAGGACGCCGGGGAGTATGTGTGTGAGACCCGGCATGAGGCCATCACCTTCAATGTCATCCTGGC
  TGAGCCTCCAGTGCAGTTCCTTGCTCTAGAGACAACTCCAAGCCCGCTCTGTGTGGCCCCTGGGGAGCCA
  GTGGTGCTGAGCTGTGAACTGTCCCGGGCTGGCGCCCCCGTGGTCTGGAGCCACAATGGGAGGCCCGTGC
  AGGAGGGCGAGGGCCTAGAGCTCCATGCCGAGGGCCCCCGCCGAGTCCTCTGCATCCAGGCTGCAGGCCC
  AGCCCATGCAGGGCTCTACACCTGCCAGTCTGGAGCAGCCCCCGGAGCCCCAAGCCTCAGCTTCACCGTC
  CAGGTGGCTGAGCCCCCTGTGCGGGTGGTAGCTCCCGAGGCAGCCCAGACGAGGGTTCGGAGCACCCCAG
  GCGGGGACCTAGAGCTGGTGGTGCACCTCTCCGGGCCAGGGGGCCCTGTACGCTGGTACAAGGACGGGGA
  GCGACTGGCAAGCCAGGGGCGGGTGCAGCTGGAGCAGGCCGGGGCCAGGCAGGTGCTGCGGGTGCAGGGG
  GCACGGAGCGGGGACGCTGGGGAGTACCTGTGCGATGCGCCCCAGGACAGCCGCATCTTCCTTGTCAGCG
  TGGAAGAGCCACTGCTGGTGAAGCTGGTCTCGGAGCTGACACCACTCACTGTCCACGAGGGCGATGATGC
  CACGTTCCGGTGTGAAGTCTCCCCACCAGATGCCGATGTCACCTGGCTGCGCAATGGGGCCGTCGTCACT
  CCAGGGCCCCAGGTGGAGATGGCCCAGAATGGTTCAAGCCGCATCTTAACCTTGCGAGGCTGCCAACTGG
  GGGATGCAGGGACCGTGACTTTGCGGGCAGGGAGCACGGCCACAAGTGCCCGGCTCCATGTTCGAGAGAC
  AGAGCTGCTGTTCCTACGGCGGTTGCAGGATGTGCGGGCAGAGGAAGGCCAGGATGTGTGTCTCGAAGTG
  GAGACAGGCCGAGTGGGTGCAGCGGGGGCCGTGCGCTGGGTGCGAGGTGGGCAGCCCCTGCCCCACGACT
  CTCGCCTGTCCATGGCCCAGGATGGGCACATCCACCGCCTCTTCATCCATGGTGTCATACTGGCCGACCA
  GGGCACCTACGGCTGCGAGAGCCACCACGATCGCACCCTGGCCAGGCTCAGCGTGAGGCCGAGGCAGCTG
  AGGGTGCTGCGGCCTCTGGAGGACGTGACCATCAGTGAGGGGGGCAGTGCCACCTTCCAGCTGGAGCTGT
  CCCAGGAAGGTGTGACCGGGGAGTGGGCCCGGGGTGGAGTACAGCTGTATCCAGGACCCAAGTGTCACAT
  CCACTCGGACGGCCACCGTCACCGACTGGTACTCAATGGCCTGGGCCTGGCCGACTCAGGCTGTGTCTCC
  TTCACAGCGGATTCCCTGCGCTGCGCAGCCAGACTCATTGTGAGAGAGGTCCCAGTGACCATCGTGCGGG
  GGCCACACGACCTAGAGGTGACCGAGGGCGACACAGCTACGTTCGAGTGCGAGCTTTCCCAAGCTTTGGC
  TGATGTTACCTGGGAGAAGGACGGGAACGCGCTTACGCCTAGCCCGCGGCTCCGGCTCCAGGCCCTCGGC
  ACGCGCCGCCTTCTCCAGCTGCGACGCTGCGGCCCCTCGGACGCCGGGACCTACAGCTGCGCGGTGGGGA
  CGGCCCGCGCCGGACCGGTCCGCCTGACCGTGCGCGAGCGTACTGTGGCGGTACTCTCCGAGCTGCGGTC
  GGTGAGCGCCCGCGAAGGCGACGGCGCTACGTTCGAGTGCACCGTGTCGGAGGTCGAGACCACGGGGCGC
  TGGGAGCTCGGAGGCCGCCCGCTGAGACCCGGAGCCCGCGTCCGCATCCGACAGGAAGGGAAGAAACACA
  TTCTGGTGCTTAGCGAGCTGCGCGCCGAGGACGCCGGTGAAGTCCGCTTCCAGGCGGGGCCCGCCCAGTC
  CCTGGCTCTACTGGAAGTGGAGGCATTGCCTCTCCAGATGTGCCGCCACCCCCCTCGCGAGAAGACCGTT
  CTGGTGGGCCGCCGGGCGGTGCTGGAGGTGACTGTGTCCCGCTCGGGGGGCCACGTGTGCTGGCTGCGGG
  AGGGGGCCGAGCTGTGCCCGGGAGATAAGTATGAGATGCGCAGCCACGGCCCCACCCACAGCCTGGTCAT
  CCATGACGTTCGACCTGAGGACCAAGGCACTTACTGCTGCCAGGCCGGCCAGGACAGCACCCACACACGG
  CTGCTGGTAGAGGGCAAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG217071 representing NM_015311
  Red=Cloning site Green=Tags(s)
  
  MKASSGDQGSPPCFLRFPRPVRVVSGAEAELKCVVLGEPPPVVVWEKGGQQLAASERLSFPADGAEHGLL
  LTAALPTDAGVYVCRARNAAGEAYAAAAVTVLEPPASDPELQPAERPLPSPGSGEGAPVFLTGPRSQWVL
  RGAEVVLTCRAGGLPEPTLYWEKDGMALDEVWDSSHFALQPGRAEDGPGASLALRILAARLPDSGVYVCH
  ARNAHGHAQAGALLQVHQPPESPPADPDEAPAPVVEPLKCAPKTFWVNEGKHAKFRCYVMGKPEPEIEWH
  WEGRPLLPDRRRLMYRDRDGGFVLKVLYCQAKDRGLYVCAARNSAGQTLSAVQLHVKEPRLRFTRPLQDV
  EGREHGIAVLECKVPNSRIPTAWFREDQRLLPCRKYEQIEEGTVRRLIIHRLKADDDGIYLCEMRGRVRT
  VANVTVKGPILKRLPRKLDVLEGENAVLLVETLEAGVEGRWSRDGEELPVICQSSSGHMHALVLPGVTRE
  DAGEVTFSLGNSRTTTLLRVKCVKHSPPGPPILAEMFKGHKNTVLLTWKPPEPAPETPFIYRLERQEVGS
  EDWIQCFSIEKAGAVEVPGDCVPSEGDYRFRICTVSGHGRSPHVVFHGSAHLVPTARLVAGLEDVQVYDG
  EDAVFSLDLSTIIQGTWFLNGEELKSNEPEGQVEPGALRYRIEQKGLQHRLILHAVKHQDSGALVGFSCP
  GVQDSAALTIQESPVHILSPQDRVSLTFTTSERVVLTCELSRVDFPATWYKDGQKVEESELLVVKMDGRK
  HRLILPEAKVQDSGEFECRTEGVSAFFGVTVQDPPVHIVDPREHVFVHAITSECVMLACEVDREDAPVRW
  YKDGQEVEESDFVVLENEGPHRRLVLPATQPSDGGEFQCVAGDECAYFTVTITDVSSWIVYPSGKVYVAA
  VRLERVVLTCELCRPWAEVRWTKDGEEVVESPALLLQKEDTVRRLVLPAVQLEDSGEYLCEIDDESASFT
  VTVTEPPVRIIYPRDEVTLIAVTLECVVLMCELSREDAPVRWYKDGLEVEESEALVLERDGPRCRLVLPA
  AQPEDGGEFVCDAGDDSAFFTVTVTAPPERIVHPAARSLDLHFGAPGRVELRCEVAPAGSQVRWYKDGLE
  VEASDALQLGAEGPTRTLTLPHAQPEDAGEYVCETRHEAITFNVILAEPPVQFLALETTPSPLCVAPGEP
  VVLSCELSRAGAPVVWSHNGRPVQEGEGLELHAEGPRRVLCIQAAGPAHAGLYTCQSGAAPGAPSLSFTV
  QVAEPPVRVVAPEAAQTRVRSTPGGDLELVVHLSGPGGPVRWYKDGERLASQGRVQLEQAGARQVLRVQG
  ARSGDAGEYLCDAPQDSRIFLVSVEEPLLVKLVSELTPLTVHEGDDATFRCEVSPPDADVTWLRNGAVVT
  PGPQVEMAQNGSSRILTLRGCQLGDAGTVTLRAGSTATSARLHVRETELLFLRRLQDVRAEEGQDVCLEV
  ETGRVGAAGAVRWVRGGQPLPHDSRLSMAQDGHIHRLFIHGVILADQGTYGCESHHDRTLARLSVRPRQL
  RVLRPLEDVTISEGGSATFQLELSQEGVTGEWARGGVQLYPGPKCHIHSDGHRHRLVLNGLGLADSGCVS
  FTADSLRCAARLIVREVPVTIVRGPHDLEVTEGDTATFECELSQALADVTWEKDGNALTPSPRLRLQALG
  TRRLLQLRRCGPSDAGTYSCAVGTARAGPVRLTVRERTVAVLSELRSVSAREGDGATFECTVSEVETTGR
  WELGGRPLRPGARVRIRQEGKKHILVLSELRAEDAGEVRFQAGPAQSLALLEVEALPLQMCRHPPREKTV
  LVGRRAVLEVTVSRSGGHVCWLREGAELCPGDKYEMRSHGPTHSLVIHDVRPEDQGTYCCQAGQDSTHTR
  LLVEGN
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015311
• ORF Size: 5688 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_015311.1, NP_056126.1
• RefSeq Size: 5841
• RefSeq ORF: 5691
• Locus ID: 23363
• Gene Summary: Cytoskeletal adaptor proteins function in linking the internal cytoskeleton of cells to the cell membrane. This gene encodes a cytoskeletal adaptor protein, which is a member of the Unc-89/obscurin family. The protein contains multiple N- and C-terminal immunoglobulin (Ig)-like domains and a central fibronectin type 3 domain. Mutations in this gene cause 3M syndrome type 2. Alternatively spliced transcript variants encoding different isoforms have been found in this gene. [provided by RefSeq, Mar 2010]