POFUT2 (NM_015227) Human Tagged ORF Clone

CAT#: RG217107

POFUT2 (GFP-tagged) - Human protein O-fucosyltransferase 2 (POFUT2), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: POFUT2
• Synonyms: C21orf80; FUT13
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG217107 representing NM_015227
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGACACTCAGCTTCGTCTTCCTGCTGCTGGGGGCAGTGTCCTGGCCTCCGGCTTCTGCCTCCGGCC
  AGGAGTTCTGGCCCGGACAATCGGCGGCCGATATTCTGTCGGGGGCGGCTTCCCGCAGACGGTATCTTCT
  GTATGACGTCAACCCCCCGGAAGGCTTCAACCTGCGCAGGGATGTCTATATCCGAATCGCCTCTCTCCTG
  AAGACTCTGCTGAAGACGGAGGAGTGGGTGCTTGTCCTGCCTCCATGGGGCCGCCTCTATCACTGGCAGA
  GTCCTGACATCCACCAGGTCCGGATTCCCTGGTCTGAGTTTTTTGATCTTCCAAGTCTCAATAAAAACAT
  CCCCGTCATCGAGTATGAGCAGTTCATCGCAGAATCTGGTGGGCCCTTTATTGACCAGGTTTACGTCCTG
  CAAAGTTACGCAGAGGGGTGGAAAGAAGGGACCTGGGAAGAGAAGGTGGACGAGCGGCCGTGTATTGATC
  AGCTCCTGTACTCCCAGGACAAGCACGAGTACTACAGAGGATGGTTTTGGGGTTATGAGGAGACCAGGGG
  TCTAAACGTCTCCTGTCTGTCCGTCCAGGGCTCAGCCTCCATCGTGGCGCCCCTGCTGCTGAGAAACACA
  TCAGCCCGGTCCGTGATGTTAGACAGAGCCGAGAACCTACTTCACGACCACTATGGAGGGAAAGAATACT
  GGGATACCCGTCGCAGCATGGTGTTTGCCAGGCACCTGCGGGAGGTGGGAGACGAGTTCAGGAGCAGACA
  TCTCAACTCCACGGACGACGCAGACAGGATCCCCTTCCAGGAGGACTGGATGAAGATGAAGGTCAAGCTG
  GGCTCCGCGCTAGGGGGCCCCTACCTGGGAGTCCACCTGAGAAGAAAAGATTTCATCTGGGGTCACAGAC
  AGGATGTACCCAGTCTGGAAGGGGCCGTGAGGAAGATCCGCAGCCTCATGAAGACCCACCGGCTGGACAA
  GGTGTTTGTGGCCACAGATGCCGTCAGAAAGGAATATGAAGAGCTAAAAAAGCTGTTACCCGAGATGGTG
  AGGTTTGAACCCACGTGGGAGGAGCTGGAGCTCTACAAGGACGGAGGCGTTGCGATTATTGACCAGTGGA
  TCTGCGCACACGCCAGGTGCCTGCCCACGTCACTGTCGGCCGAGAGCGGGTCGGGTGGCTTTCAAAGGTT
  CTTCTGTCCCAAGTACTCGGTGTCAGAGCAGATGGTCGCCTGTGTTCACAGTGGTCATTTCCATACTGTT
  TGCCTCCTCGTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG217107 representing NM_015227
  Red=Cloning site Green=Tags(s)
  
  MATLSFVFLLLGAVSWPPASASGQEFWPGQSAADILSGAASRRRYLLYDVNPPEGFNLRRDVYIRIASLL
  KTLLKTEEWVLVLPPWGRLYHWQSPDIHQVRIPWSEFFDLPSLNKNIPVIEYEQFIAESGGPFIDQVYVL
  QSYAEGWKEGTWEEKVDERPCIDQLLYSQDKHEYYRGWFWGYEETRGLNVSCLSVQGSASIVAPLLLRNT
  SARSVMLDRAENLLHDHYGGKEYWDTRRSMVFARHLREVGDEFRSRHLNSTDDADRIPFQEDWMKMKVKL
  GSALGGPYLGVHLRRKDFIWGHRQDVPSLEGAVRKIRSLMKTHRLDKVFVATDAVRKEYEELKKLLPEMV
  RFEPTWEELELYKDGGVAIIDQWICAHARCLPTSLSAESGSGGFQRFFCPKYSVSEQMVACVHSGHFHTV
  CLLV
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015227
• ORF Size: 1272 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_015227.3, NP_056042.1
• RefSeq Size: 4828
• RefSeq ORF: 1275
• Locus ID: 23275
• Gene Summary: Fucose is typically found as a terminal modification of branched chain glycoconjugates, but it also exists in direct O-linkage to serine or threonine residues within cystine knot motifs in epidermal growth factor (EGF; MIM 131530)-like repeats or thrombospondin (THBS; see MIM 188060) type-1 repeats. POFUT2 is an O-fucosyltransferase that use THBS type-1 repeats as substrates (Luo et al., 2006 [PubMed 16464857]). [supplied by OMIM, Mar 2008]