RUFY1 (NM_025158) Human Tagged ORF Clone

CAT#: RG217181

RUFY1 (GFP-tagged) - Human RUN and FYVE domain containing 1 (RUFY1), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: RUFY1
• Synonyms: RABIP4; ZFYVE12
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG217181 representing NM_025158
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGACCGGGAAGGCGGCTGCGCTGCTGGGCGGGGGCGGGAGCTGGAGCCGGAGCTGGAGCCGGGGC
  CGGGGCCCGGGTCAGCGCTTGAGCCGGGAGAAGAGTTTGAGATCGTGGACCGAAGCCAGCTGCCCGGCCC
  AGGCGACCTGCGGAGCGCAACGAGGCCGCGGGCGGCCGAGGGCTGGTCGGCGCCCATCCTGACCCTGGCA
  CGCAGGGCCACCGGGAACCTGTCGGCGAGCTGCGGGAGCGCGCTGCGCGCGGCCGCGGGGCTGGGCGGCG
  GGGACAGCGGGGACGGCACGGCGCGCGCAGCTTCTAAGTGCCAGATGATGGAGGAGCGTGCCAACCTGAT
  GCACATGATGAAACTCAGCATCAAGGTGTTGCTCCAGTCGGCTCTGAGCCTGGGCCGCAGCCTGGATGCG
  GACCATGCCCCCTTGCAGCAGTTCTTTGTAGTGATGGAGCACTGCCTCAAACATGGGCTGAAAGTTAAGA
  AGAGTTTTATTGGCCAAAATAAATCATTCTTTGGTCCTTTGGAGCTGGTGGAGAAACTTTGTCCAGAAGC
  ATCAGATATAGCGACTAGTGTCAGAAATCTTCCAGAATTAAAGACAGCTGTGGGAAGAGGCCGAGCGTGG
  CTTTATCTTGCACTCATGCAAAAGAAACTGGCAGATTATCTGAAAGTGCTTATAGACAATAAACATCTCT
  TAAGCGAGTTCTATGAGCCTGAGGCTTTAATGATGGAGGAAGAAGGGATGGTGATTGTTGGTCTGCTGGT
  GGGACTCAATGTTCTCGATGCCAATCTCTGCTTGAAAGGAGAAGACTTGGATTCTCAGGTTGGAGTAATA
  GATTTTTCCCTCTACCTTAAGGATGTGCAGGATCTTGATGGTGGCAAGGAGCATGAAAGAATTACTGATG
  TCCTTGATCAAAAAAATTATGTGGAAGAACTTAACCGGCACTTGAGCTGCACAGTTGGGGATCTTCAAAC
  CAAGATAGATGGCTTGGAAAAGACTAACTCAAAGCTTCAAGAAGAGCTTTCAGCTGCAACAGACCGAATT
  TGCTCACTTCAAGAAGAACAGCAGCAGTTAAGAGAACAAAATGAATTAATTCGAGAAAGAAGTGAAAAGA
  GTGTAGAGATAACAAAACAGGATACCAAAGTTGAGCTGGAGACTTACAAGCAAACTCGGCAAGGTCTGGA
  TGAAATGTACAGTGATGTGTGGAAGCAGCTAAAAGAGGAGAAGAAAGTCCGGTTGGAACTGGAAAAAGAA
  CTGGAGTTACAAATTGGAATGAAAACCGAAATGGAAATTGCAATGAAGTTACTGGAAAAGGACACCCACG
  AGAAGCAGGACACACTAGTTGCCCTCCGCCAGCAGCTGGAAGAAGTCAAAGCGATTAATTTACAGATGTT
  TCACAAAGCTCAGAATGCAGAGAGCAGTTTGCAGCAGAAGAATGAAGCCATCACATCCTTTGAAGGAAAA
  ACCAACCAAGTTATGTCCAGCATGAAACAAATGGAAGAAAGGTTGCAGCACTCGGAGCGGGCGAGGCAGG
  GGGCTGAGGAGCGGAGCCACAAGCTGCAGCAGGAGCTGGGCGGGAGGATCGGCGCCCTGCAGCTGCAGCT
  CTCCCAGCTGCACGAGCAATGCTCAAGCCTGGAGAAAGAATTGAAATCAGAAAAAGAGCAAAGACAGGCT
  CTTCAGCGCGAATTACAGCACGAGAAAGACACTTCCTCTCTACTCAGGATGGAGCTGCAACAAGTGGAAG
  GACTGAAAAAGGAGTTGCGGGAGCTTCAGGACGAGAAGGCAGAGCTGCAGAAGATCTGCGAGGAGCAGGA
  ACAAGCCCTCCAGGAAATGGGCCTGCACCTCAGCCAGTCCAAGCTGAAGATGGAAGATATAAAAGAAGTG
  AACCAGGCACTGAAGGGCCACGCCTGGCTGAAAGATGACGAAGCGACACACTGTAGGCAGTGTGAGAAGG
  AGTTCTCCATTTCCCGGAGAAAGCACCACTGCCGGAACTGTGGCCACATCTTCTGCAACACCTGCTCCAG
  CAACGAGCTGGCCCTGCCCTCCTACCCCAAGCCGGTGCGAGTGTGCGACAGCTGCCACACCCTGCTCCTG
  CAGCGCTGCTCCTCCACGGCCTCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG217181 representing NM_025158
  Red=Cloning site Green=Tags(s)
  
  MADREGGCAAGRGRELEPELEPGPGPGSALEPGEEFEIVDRSQLPGPGDLRSATRPRAAEGWSAPILTLA
  RRATGNLSASCGSALRAAAGLGGGDSGDGTARAASKCQMMEERANLMHMMKLSIKVLLQSALSLGRSLDA
  DHAPLQQFFVVMEHCLKHGLKVKKSFIGQNKSFFGPLELVEKLCPEASDIATSVRNLPELKTAVGRGRAW
  LYLALMQKKLADYLKVLIDNKHLLSEFYEPEALMMEEEGMVIVGLLVGLNVLDANLCLKGEDLDSQVGVI
  DFSLYLKDVQDLDGGKEHERITDVLDQKNYVEELNRHLSCTVGDLQTKIDGLEKTNSKLQEELSAATDRI
  CSLQEEQQQLREQNELIRERSEKSVEITKQDTKVELETYKQTRQGLDEMYSDVWKQLKEEKKVRLELEKE
  LELQIGMKTEMEIAMKLLEKDTHEKQDTLVALRQQLEEVKAINLQMFHKAQNAESSLQQKNEAITSFEGK
  TNQVMSSMKQMEERLQHSERARQGAEERSHKLQQELGGRIGALQLQLSQLHEQCSSLEKELKSEKEQRQA
  LQRELQHEKDTSSLLRMELQQVEGLKKELRELQDEKAELQKICEEQEQALQEMGLHLSQSKLKMEDIKEV
  NQALKGHAWLKDDEATHCRQCEKEFSISRRKHHCRNCGHIFCNTCSSNELALPSYPKPVRVCDSCHTLLL
  QRCSSTAS
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_025158
• ORF Size: 2124 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_025158.3, NP_079434.3
• RefSeq Size: 2644
• RefSeq ORF: 2127
• Locus ID: 80230
• Domains: FYVE, RUN
• Protein Pathways: Endocytosis
• Gene Summary: This gene encodes a protein that contains a RUN domain and a FYVE-type zinc finger domain. The encoded protein binds to phosphatidylinositol-3-phosphate (PI3P) and plays a role in early endosomal trafficking, tethering and fusion through interactions with small GTPases including Rab4, Rab5 and Rab14. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2011]