POFUT1 (NM_015352) Human Tagged ORF Clone

CAT#: RG217185

POFUT1 (GFP-tagged) - Human protein O-fucosyltransferase 1 (POFUT1), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: POFUT1
• Synonyms: DDD2; FUT12; O-Fuc-T; O-FucT-1; O-FUT; OFUCT1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG217185 representing NM_015352
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCGCCGCCGCGTGGGCACGGCCGCTGAGCGTGTCTTTCCTGCTGCTGCTTCTGCCGCTCCCGGGGA
  TGCCTGCGGGCTCCTGGGACCCGGCCGGTTACCTGCTCTACTGCCCCTGCATGGGGCGCTTTGGGAACCA
  GGCCGATCACTTCTTGGGCTCTCTGGCATTTGCAAAGCTGCTAAACCGTACCTTGGCTGTCCCTCCTTGG
  ATTGAGTACCAGCATCACAAGCCTCCTTTCACCAACCTCCATGTGTCCTACCAGAAGTACTTCAAGCTGG
  AGCCCCTCCAGGCTTACCATCGGGTCATCAGCTTGGAGGATTTCATGGAGAAGCTGGCACCCACCCACTG
  GCCCCCTGAGAAGCGGGTGGCATACTGCTTTGAGGTGGCAGCCCAGCGAAGCCCAGATAAGAAGACGTGC
  CCCATGAAGGAAGGAAACCCCTTTGGCCCATTCTGGGATCAGTTTCATGTGAGTTTCAACAAGTCGGAGC
  TTTTTACAGGCATTTCCTTCAGTGCTTCCTACAGAGAACAATGGAGCCAGAGATTTTCTCCAAAGGAACA
  TCCGGTGCTTGCCCTGCCAGGAGCCCCAGCCCAGTTCCCCGTCCTAGAGGAACACAGGCCACTACAGAAG
  TACATGGTATGGTCAGACGAAATGGTGAAGACGGGAGAGGCCCAGATTCATGCCCACCTTGTCCGGCCCT
  ATGTGGGCATTCATCTGCGCATTGGCTCTGACTGGAAGAACGCCTGTGCCATGCTGAAGGACGGGACTGC
  AGGCTCGCACTTCATGGCCTCTCCGCAGTGTGTGGGCTACAGCCGCAGCACAGCGGCCCCCCTCACGATG
  ACTATGTGCCTGCCTGACCTGAAGGAGATCCAGAGGGCTGTGAAGCTCTGGGTGAGGTCGCTGGATGCCC
  AGTCGGTCTACGTTGCTACTGATTCCGAGAGTTATGTGCCTGAGCTCCAACAGCTCTTCAAAGGGAAGGT
  GAAGGTGGTGAGCCTGAAGCCTGAGGTGGCCCAGGTCGACCTGTACATCCTCGGCCAAGCCGACCACTTT
  ATTGGCAACTGTGTCTCCTCCTTCACTGCCTTTGTGAAGCGGGAGCGGGACCTCCAGGGGAGGCCGTCTT
  CTTTCTTCGGCATGGACAGGCCCCCTAAGCTGCGGGACGAGTTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG217185 representing NM_015352
  Red=Cloning site Green=Tags(s)
  
  MGAAAWARPLSVSFLLLLLPLPGMPAGSWDPAGYLLYCPCMGRFGNQADHFLGSLAFAKLLNRTLAVPPW
  IEYQHHKPPFTNLHVSYQKYFKLEPLQAYHRVISLEDFMEKLAPTHWPPEKRVAYCFEVAAQRSPDKKTC
  PMKEGNPFGPFWDQFHVSFNKSELFTGISFSASYREQWSQRFSPKEHPVLALPGAPAQFPVLEEHRPLQK
  YMVWSDEMVKTGEAQIHAHLVRPYVGIHLRIGSDWKNACAMLKDGTAGSHFMASPQCVGYSRSTAAPLTM
  TMCLPDLKEIQRAVKLWVRSLDAQSVYVATDSESYVPELQQLFKGKVKVVSLKPEVAQVDLYILGQADHF
  IGNCVSSFTAFVKRERDLQGRPSSFFGMDRPPKLRDEF
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015352
• ORF Size: 1164 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_015352.1, NP_056167.1
• RefSeq Size: 5249
• RefSeq ORF: 1167
• Locus ID: 23509
• Protein Families: Druggable Genome, Transcription Factors
• Gene Summary: This gene encodes a member of the glycosyltransferase O-Fuc family. This enzyme adds O-fucose through an O-glycosidic linkage to conserved serine or threonine residues in the epidermal growth factor-like repeats of a number of cell surface and secreted proteins. O-fucose glycans are involved in ligand-induced receptor signaling. Alternative splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]