KIF1B (NM_015074) Human Tagged ORF Clone

CAT#: RG217365

KIF1B (GFP-tagged) - Human kinesin family member 1B (KIF1B), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: KIF1B
• Synonyms: CMT2; CMT2A; CMT2A1; HMSNII; KLP; NBLST1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG217365 representing NM_015074
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGGGAGCCTCAGTGAAGGTGGCTGTCCGGGTAAGGCCCTTCAATTCTCGAGAGACCAGCAAGGAAT
  CCAAATGCATCATTCAGATGCAAGGCAACTCGACCAGTATTATTAACCCAAAGAATCCAAAGGAAGCTCC
  AAAGTCCTTCAGCTTCGACTATTCCTACTGGTCTCATACCTCACCCGAAGATCCCTGTTTTGCATCTCAA
  AACCGTGTGTACAATGACATTGGCAAGGAAATGCTCTTACACGCCTTTGAGGGATATAATGTCTGTATTT
  TTGCCTATGGGCAGACTGGTGCTGGAAAATCTTATACAATGATGGGTAAACAAGAAGAAAGCCAGGCTGG
  CATCATTCCACAGTTATGTGAAGAACTTTTTGAGAAAATCAATGACAACTGTAATGAAGAAATGTCTTAC
  TCTGTAGAGGTGAGCTACATGGAAATTTACTGTGAAAGAGTACGAGATTTGCTGAATCCAAAAAACAAGG
  GTAATTTGCGTGTGCGTGAACACCCACTTCTTGGACCCTATGTGGAGGATCTGTCCAAGTTGGCAGTTAC
  TTCCTACACAGACATTGCTGACCTCATGGATGCTGGGAACAAAGCCAGGACAGTGGCAGCTACAAACATG
  AATGAAACAAGTAGCCGTTCCCACGCTGTGTTTACGATTGTTTTCACCCAGAAGAAACACGATAATGAGA
  CCAACCTTTCCACTGAGAAGGTCAGTAAAATCAGCTTGGTGGATCTAGCAGGAAGTGAACGAGCTGATTC
  AACTGGTGCCAAAGGGACTCGATTAAAGGAAGGAGCAAATATTAATAAGTCTCTTACAACTTTGGGCAAA
  GTCATTTCAGCCTTGGCCGAGGTGAGTAAAAAGAAGAAGAAAACAGATTTTATTCCCTACAGGGATTCTG
  TACTTACTTGGCTCCTTCGAGAAAATTTAGGTGGCAATTCTCGGACTGCAATGGTTGCTGCTCTGAGCCC
  CGCGGATATCAACTACGATGAGACTTTGAGCACTCTGAGATATGCAGATCGTGCAAAACAAATTAAATGC
  AATGCTGTTATCAATGAGGACCCCAATGCCAAACTGGTTCGTGAATTAAAGGAGGAGGTGACACGGCTGA
  AGGACCTTCTTCGTGCTCAGGGCCTGGGAGATATTATTGATACATCCATGGGGTCCCTCACTTCATCCCC
  ATCTTCCTGCTCACTCAGTAGTCAGGTGGGCTTGACGTCTGTGACCAGTATTCAAGAGAGGATCATGTCT
  ACACCTGGAGGAGAGGAAGCTATTGAACGTTTAAAGGAATCAGAGAAGATCATTGCTGAGTTGAATGAAA
  CTTGGGAAGAGAAGCTTCGTAAAACAGAGGCCATCAGAATGGAGAGAGAGGCTTTGTTGGCTGAGATGGG
  AGTTGCCATTCGGGAAGATGGAGGAACCCTAGGGGTTTTCTCACCTAAAAAGACCCCACATCTTGTTAAC
  CTCAATGAAGACCCACTAATGTCTGAGTGCCTACTTTATTACATCAAAGATGGAATTACAAGGGTTGGCC
  AAGCAGATGCTGAGCGGCGCCAGGACATAGTGCTGAGCGGGGCTCACATTAAAGAAGAGCATTGTATCTT
  CCGGAGTGAGAGAAGCAACAGCGGGGAAGTTATCGTGACCTTAGAGCCCTGTGAGCGCTCAGAAACCTAC
  GTAAATGGCAAGAGGGTGTCCCAGCCTGTTCAGCTGCGCTCAGGAAACCGTATCATCATGGGTAAAAACC
  ATGTTTTCCGCTTTAACCACCCGGAACAAGCACGAGCTGAGCGAGAGAAGACTCCTTCTGCTGAGACCCC
  CTCTGAGCCTGTGGACTGGACATTTGCCCAGAGGGAGCTTCTGGAAAAACAAGGAATTGATATGAAACAA
  GAGATGGAGAAAAGGCTACAGGAAATGGAGATCTTATACAAAAAGGAGAAGGAAGAAGCAGATCTTCTTT
  TGGAGCAGCAGAGACTGGACTATGAGAGTAAATTGCAGGCCTTGCAGAAGCAGGTTGAAACCCGATCTCT
  GGCTGCAGAAACAACTGAAGAGGAGGAAGAAGAGGAAGAAGTTCCTTGGACACAGCATGAATTTGAGTTG
  GCCCAATGGGCCTTCCGGAAATGGAAGTCTCATCAGTTTACTTCATTACGGGACTTACTCTGGGGCAATG
  CCGTGTACCTAAAGGAGGCCAATGCCATCAGTGTGGAACTGAAAAAGAAGGTGCAGTTTCAGTTTGTTCT
  GCTGACTGACACACTGTACTCCCCTTTGCCTCCTGAATTACTTCCCACTGAGATGGAAAAAACTCATGAG
  GACAGGCCTTTCCCTCGCACAGTGGTAGCAGTAGAAGTCCAGGATTTGAAGAATGGAGCAACACACTATT
  GGTCTTTGGAGAAACTCAAGCAGAGGCTGGATTTGATGCGAGAGATGTATGATAGGGCAGGGGAGATGGC
  CTCCAGTGCCCAAGACGAAAGCGAAACCACTGTGACTGGCAGCGATCCCTTCTATGATCGGTTCCACTGG
  TTCAAACTTGTGGGGAGCTCCCCCATTTTCCACGGCTGTGTGAACGAGCGCCTTGCCGACCGCACACCCT
  CCCCCACTTTTTCCACGGCCGATTCCGACATCACTGAGCTGGCTGACGAGCAGCAAGATGAGATGGAGGA
  TTTTGATGATGAGGCATTCGTGGATGACGCCGGCTCTGACGCAGGGACGGAGGAGGGATCAGATCTCTTC
  AGTGACGGGCATGACCCGTTTTACGACCGATCCCCTTGGTTCATTTTAGTGGGAAGGGCATTTGTTTACC
  TGAGCAATCTGCTGTATCCCGTGCCCCTGATCCACAGGGTGGCCATCGTCAGTGAGAAAGGTGAAGTGCG
  GGGATTTCTGCGTGTGGCTGTACAGGCCATCGCAGCGGATGAAGAAGCTCCTGATTATGGCTCTGGAATT
  CGACAGTCAGGAACAGCTAAAATATCTTTTGATAATGAATACTTTAATCAGAGTGACTTTTCGTCTGTTG
  CAATGACTCGTTCTGGTCTGTCCTTGGAGGAGTTGAGGATTGTGGAAGGACAGGGTCAGAGTTCTGAGGT
  CATCACTCCTCCAGAAGAAATCAGTCGAATTAATGACTTGGATTTGAAGTCAAGCACTTTGCTGGATGGT
  AAGATGGTAATGGAAGGGTTTTCTGAAGAGATTGGCAACCACCTGAAACTGGGCAGTGCCTTCACTTTCC
  GAGTAACAGTGTTGCAGGCCAGTGGAATCCTCCCAGAGTATGCAGATATCTTCTGTCAGTTCAACTTTTT
  GCATCGCCATGATGAAGCATTCTCCACGGAGCCCCTCAAAAACAATGGCAGAGGAAGTCCCCTGGCCTTT
  TATCATGTGCAGAATATTGCAGTGGAGATCACTGAATCATTTGTGGATTACATCAAAACCAAGCCTATTG
  TATTTGAAGTCTTTGGGCATTATCAGCAGCACCCACTTCATCTGCAAGGACAGGAGCTTAACAGTCCGCC
  TCAGCCGTGCCGCCGATTCTTCCCTCCACCCATGCCACTGTCCAAGCCAGTTCCAGCCACCAAGTTAAAC
  ACGATGAGCAAAACCAGCCTTGGCCAGAGCATGAGCAAGTATGACCTCCTGGTTTGGTTTGAGATCAGTG
  AACTGGAGCCTACAGGAGAGTATATCCCAGCTGTGGTTGACCACACAGCAGGCTTGCCTTGCCAGGGGAC
  ATTTTTGCTTCATCAGGGCATCCAGCGAAGGATCACAGTGACCATTATCCATGAGAAGGGGAGCGAGCTC
  CATTGGAAAGATGTTCGTGAACTGGTGGTAGGTCGTATTCGGAATAAGCCTGAGGTGGATGAAGCTGCAG
  TTGATGCCATCCTCTCCCTAAATATTATTTCTGCCAAGTACCTGAAGTCTTCCCACAACTCTAGCAGGAC
  CTTCTACCGCTTTGAGGCTGTGTGGGATAGCTCTCTGCATAACTCCCTTCTTCTGAACCGAGTGACACCC
  TATGGAGAAAAGATCTACATGACCTTGTCGGCCTACCTAGAGCTGGATCATTGCATCCAGCCGGCTGTCA
  TCACCAAGGATGTGTGCATGGTCTTCTACTCCCGAGATGCCAAGATCTCACCACCACGCTCTCTGCGTAG
  CCTCTTTGGCAGCGGCTACTCAAAGTCACCAGATTCGAATCGAGTCACTGGCATTTACGAACTCAGCTTA
  TGCAAAATGTCAGACACAGGTAGTCCAGGTATGCAGAGAAGGAGAAGAAAAATCTTAGATACGTCAGTGG
  CATATGTGCGGGGAGAAGAGAACTTAGCAGGCTGGCGGCCCCGTGGAGACAGCCTCATCCTTGAGCACCA
  GTGGGAGCTGGAGAAGCTGGAGCTCCTACATGAGGTGGAAAAAACCCGCCACTTTTTGCTGCTGCGTGAG
  AGACTTGGTGACAGCATCCCCAAATCCCTGAGCGACTCGTTATCCCCCAGCCTCAGCAGTGGGACCCTCA
  GCACCTCCACCAGTATCTCCTCTCAGATCTCAACCACTACCTTTGAAAGCGCCATCACACCTAGCGAGAG
  CAGTGGCTATGATTCAGGAGACATCGAAAGCCTGGTGGACCGAGAGAAAGAGCTGGCTACCAAGTGCCTG
  CAACTTCTCACCCACACTTTCAACAGAGAATTCAGCCAGGTGCACGGCAGCGTCAGTGACTGTAAGTTGT
  CTGATATCTCTCCAATTGGACGGGATCCCTCTGAGTCCAGTTTCAGCAGTGCCACCCTCACTCCCTCCTC
  CACCTGTCCCTCTCTGGTAGACTCTAGGAGCAACTCTCTGGATCAGAAGACCCCAGAAGCCAATTCCCGG
  GCCTCTAGTCCCTGCCCAGAATTTGAACAGTTTCAGATTGTCCCAGCTGTGGAAACACCATATTTGGCCC
  GAGCAGGAAAAAACGAATTTCTCAATCTTGTTCCAGATATTGAAGAAATTAGACCAAGCTCAGTGGTCTC
  TAAGAAAGGATACCTTCATTTCAAGGAGCCTCTTTACAGTAACTGGGCTAAACATTTTGTTGTCGTCCGT
  CGGCCTTATGTCTTCATCTATAACAGTGACAAAGACCCTGTGGAGCGTGGAATCATTAACCTGTCCACAG
  CACAGGTGGAGTACAGTGAGGACCAGCAGGCCATGGTGAAGACACCAAACACCTTTGCTGTCTGCACAAA
  GCACCGTGGGGTCCTTTTGCAGGCCCTCAATGACAAAGACATGAACGACTGGTTGTATGCCTTCAACCCA
  CTTCTAGCTGGCACAATACGGTCAAAGCTTTCCCGCAGATGCCCGAGCCAGTCGAAATAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG217365 representing NM_015074
  Red=Cloning site Green=Tags(s)
  
  MSGASVKVAVRVRPFNSRETSKESKCIIQMQGNSTSIINPKNPKEAPKSFSFDYSYWSHTSPEDPCFASQ
  NRVYNDIGKEMLLHAFEGYNVCIFAYGQTGAGKSYTMMGKQEESQAGIIPQLCEELFEKINDNCNEEMSY
  SVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYTDIADLMDAGNKARTVAATNM
  NETSSRSHAVFTIVFTQKKHDNETNLSTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGK
  VISALAEVSKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIKC
  NAVINEDPNAKLVRELKEEVTRLKDLLRAQGLGDIIDTSMGSLTSSPSSCSLSSQVGLTSVTSIQERIMS
  TPGGEEAIERLKESEKIIAELNETWEEKLRKTEAIRMEREALLAEMGVAIREDGGTLGVFSPKKTPHLVN
  LNEDPLMSECLLYYIKDGITRVGQADAERRQDIVLSGAHIKEEHCIFRSERSNSGEVIVTLEPCERSETY
  VNGKRVSQPVQLRSGNRIIMGKNHVFRFNHPEQARAEREKTPSAETPSEPVDWTFAQRELLEKQGIDMKQ
  EMEKRLQEMEILYKKEKEEADLLLEQQRLDYESKLQALQKQVETRSLAAETTEEEEEEEEVPWTQHEFEL
  AQWAFRKWKSHQFTSLRDLLWGNAVYLKEANAISVELKKKVQFQFVLLTDTLYSPLPPELLPTEMEKTHE
  DRPFPRTVVAVEVQDLKNGATHYWSLEKLKQRLDLMREMYDRAGEMASSAQDESETTVTGSDPFYDRFHW
  FKLVGSSPIFHGCVNERLADRTPSPTFSTADSDITELADEQQDEMEDFDDEAFVDDAGSDAGTEEGSDLF
  SDGHDPFYDRSPWFILVGRAFVYLSNLLYPVPLIHRVAIVSEKGEVRGFLRVAVQAIAADEEAPDYGSGI
  RQSGTAKISFDNEYFNQSDFSSVAMTRSGLSLEELRIVEGQGQSSEVITPPEEISRINDLDLKSSTLLDG
  KMVMEGFSEEIGNHLKLGSAFTFRVTVLQASGILPEYADIFCQFNFLHRHDEAFSTEPLKNNGRGSPLAF
  YHVQNIAVEITESFVDYIKTKPIVFEVFGHYQQHPLHLQGQELNSPPQPCRRFFPPPMPLSKPVPATKLN
  TMSKTSLGQSMSKYDLLVWFEISELEPTGEYIPAVVDHTAGLPCQGTFLLHQGIQRRITVTIIHEKGSEL
  HWKDVRELVVGRIRNKPEVDEAAVDAILSLNIISAKYLKSSHNSSRTFYRFEAVWDSSLHNSLLLNRVTP
  YGEKIYMTLSAYLELDHCIQPAVITKDVCMVFYSRDAKISPPRSLRSLFGSGYSKSPDSNRVTGIYELSL
  CKMSDTGSPGMQRRRRKILDTSVAYVRGEENLAGWRPRGDSLILEHQWELEKLELLHEVEKTRHFLLLRE
  RLGDSIPKSLSDSLSPSLSSGTLSTSTSISSQISTTTFESAITPSESSGYDSGDIESLVDREKELATKCL
  QLLTHTFNREFSQVHGSVSDCKLSDISPIGRDPSESSFSSATLTPSSTCPSLVDSRSNSLDQKTPEANSR
  ASSPCPEFEQFQIVPAVETPYLARAGKNEFLNLVPDIEEIRPSSVVSKKGYLHFKEPLYSNWAKHFVVVR
  RPYVFIYNSDKDPVERGIINLSTAQVEYSEDQQAMVKTPNTFAVCTKHRGVLLQALNDKDMNDWLYAFNP
  LLAGTIRSKLSRRCPSQSKY
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015074
• ORF Size: 5310 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_015074.3, NP_055889.2
• RefSeq Size: 10585
• RefSeq ORF: 5313
• Locus ID: 23095
• Domains: FHA, kinesin, PH
• Protein Families: Druggable Genome
• Gene Summary: This gene encodes a motor protein that transports mitochondria and synaptic vesicle precursors. Mutations in this gene cause Charcot-Marie-Tooth disease, type 2A1. [provided by RefSeq, Jul 2008]