PRMT4 (CARM1) (NM_199141) Human Tagged ORF Clone

CAT#: RG217483

CARM1 (GFP-tagged) - Human coactivator-associated arginine methyltransferase 1 (CARM1)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: CARM1
• Synonyms: PRMT4
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG217483 representing NM_199141
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGCGGCGGCGGCGGCGGTGGGGCCGGGCGCGGGCGGCGCGGGGTCGGCGGTCCCGGGCGGCGCGG
  GGCCCTGCGCTACCGTGTCGGTGTTCCCCGGCGCCCGCCTCCTCACCATCGGCGACGCGAACGGCGAGAT
  CCAGCGGCACGCGGAGCAGCAGGCGCTGCGCCTCGAGGTGCGCGCCGGCCCGGACTCGGCGGGCATCGCC
  CTCTACAGCCATGAAGATGTGTGTGTCTTTAAGTGCTCAGTGTCCCGAGAGACAGAGTGCAGCCGTGTGG
  GCAAGCAGTCCTTCATCATCACCCTGGGCTGCAACAGCGTCCTCATCCAGTTCGCCACACCCAACGATTT
  CTGTTCCTTCTACAACATCCTGAAAACCTGCCGGGGCCACACCCTGGAGCGGTCTGTGTTCAGCGAGCGG
  ACGGAGGAGTCTTCTGCCGTGCAGTACTTCCAGTTTTATGGCTACCTGTCCCAGCAGCAGAACATGATGC
  AGGACTACGTGCGGACAGGCACCTACCAGCGCGCCATCCTGCAAAACCACACCGACTTCAAGGACAAGAT
  CGTTCTTGATGTTGGCTGTGGCTCTGGGATCCTGTCGTTTTTTGCCGCCCAAGCTGGAGCACGGAAAATC
  TACGCGGTGGAGGCCAGCACCATGGCCCAGCACGCTGAGGTCTTGGTGAAGAGTAACAACCTGACGGACC
  GCATCGTGGTCATCCCGGGCAAGGTGGAGGAGGTGTCACTCCCCGAGCAGGTGGACATCATCATCTCGGA
  GCCCATGGGCTACATGCTCTTCAACGAGCGCATGCTGGAGAGCTACCTCCACGCCAAGAAGTACCTGAAG
  CCCAGCGGAAACATGTTTCCTACCATTGGTGACGTCCACCTTGCACCCTTCACGGATGAACAGCTCTACA
  TGGAGCAGTTCACCAAGGCCAACTTCTGGTACCAGCCATCTTTCCATGGAGTGGACCTGTCGGCCCTCCG
  AGGTGCCGCGGTGGATGAGTATTTCCGGCAGCCTGTGGTGGACACATTTGACATCCGGATCCTGATGGCC
  AAGTCTGTCAAGTACACGGTGAACTTCTTAGAAGCCAAAGAAGGAGATTTGCACAGGATAGAAATCCCAT
  TCAAATTCCACATGCTGCATTCAGGGCTGGTCCACGGCCTGGCTTTCTGGTTTGACGTTGCTTTCATCGG
  CTCCATAATGACCGTGTGGCTGTCCACAGCCCCGACAGAGCCCCTGACCCACTGGTACCAGGTGCGGTGC
  CTGTTCCAGTCACCACTGTTCGCCAAGGCAGGGGACACGCTCTCAGGGACATGTCTGCTTATTGCCAACA
  AAAGACAGAGCTACGACATCAGTATTGTGGCCCAGGTGGACCAGACCGGCTCCAAGTCCAGTAACCTCCT
  GGATCTGAAAAACCCCTTCTTTAGATACACGGGCACAACGCCCTCACCCCCACCCGGCTCCCACTACACA
  TCTCCCTCGGAAAACATGTGGAACACGGGCAGCACCTACAACCTCAGCAGCGGGATGGCCGTGGCAGGGA
  TGCCGACCGCCTATGACTTGAGCAGTGTTATTGCCAGTGGCTCCAGCGTGGGCCACAACAACCTGATTCC
  TTTAGCCAACACGGGGATTGTCAATCACACCCACTCCCGGATGGGCTCCATAATGAGCACGGGGATTGTC
  CAAGGGTCCTCCGGCGCCCAGGGCAGTGGTGGTGGCAGCACGAGTGCCCACTATGCAGTCAACAGCCAGT
  TCACCATGGGCGGCCCCGCCATCTCCATGGCGTCGCCCATGTCCATCCCGACCAACACCATGCACTACGG
  GAGC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_199141
• ORF Size: 1824 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_199141.1, NP_954592.1
• RefSeq Size: 2968
• RefSeq ORF: 1827
• Locus ID: 10498
• Protein Families: Druggable Genome, Transcription Factors
• Gene Summary: This gene belongs to the protein arginine methyltransferase (PRMT) family. The encoded enzyme catalyzes the methylation of guanidino nitrogens of arginyl residues of proteins. The enzyme acts specifically on histones and other chromatin-associated proteins and is involved in regulation of gene expression. The enzyme may act in association with other proteins or within multi-protein complexes and may play a role in cell type-specific functions and cell lineage specification. A related pseudogene is located on chromosome 9. [provided by RefSeq, Aug 2013]