Syntaxin 2 (STX2) (NM_194356) Human Tagged ORF Clone

CAT#: RG217529

STX2 (GFP-tagged) - Human syntaxin 2 (STX2), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: STX2
• Synonyms: EPIM; EPM; STX2A; STX2B; STX2C
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG217529 representing NM_194356
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGGGACCGGCTGCCAGACCTGACGGCGTGTAGGAAGAATGATGATGGAGACACAGTTGTTGTGGTTG
  AGAAAGATCATTTCATGGATGATTTCTTCCATCAGGTGGAGGAGATTAGAAACACTATTGATAAAATAAC
  TCAATATGTTGAAGAAGTAAAGAAAAACCACAGCATCATTCTTTCTGCACCAAACCCGGAAGGAAAAATA
  AAAGAAGAGCTTGAAGATCTGAACAAAGAAATCAAGAAAACTGCGAATAAAATTCGAGCCAAGTTAAAGG
  CTATTGAACAAAGTTTTGATCAGGATGAGAGTGGGAACCGGACTTCAGTGGATCTTCGGATACGAAGAAC
  CCAGCATTCGGTGCTGTCTCGGAAGTTTGTGGAAGCCATGGCGGAGTACAATGAGGCACAGACTCTGTTT
  CGGGAGCGGAGCAAAGGCCGCATCCAGCGCCAGCTGGAGATAACTGGGAGAACCACCACAGACGACGAGC
  TAGAAGAGATGCTGGAGAGCGGGAAGCCATCCATCTTCACTTCCGACATTATATCAGATTCACAAATTAC
  TAGACAAGCTCTCAATGAAATCGAGTCACGTCACAAGGACATCATGAAGCTGGAGACCAGCATCCGAGAG
  TTGCATGAGATGTTCATGGACATGGCTATGTTTGTGGAGACTCAGGGTGAAATGATCAACAACATAGAAA
  GAAATGTTATGAATGCCACAGACTATGTAGAACACGCTAAAGAAGAAACAAAAAAAGCTATCAAATATCA
  GAGCAAGGCAAGAAGGAAAAAGTGGATAATTATTGCTGTGTCAGTGGTTCTGGTTGCCATAATCGCTCTA
  ATTATTGGCTTGTCAGTTGGCAAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG217529 representing NM_194356
  Red=Cloning site Green=Tags(s)
  
  MRDRLPDLTACRKNDDGDTVVVVEKDHFMDDFFHQVEEIRNTIDKITQYVEEVKKNHSIILSAPNPEGKI
  KEELEDLNKEIKKTANKIRAKLKAIEQSFDQDESGNRTSVDLRIRRTQHSVLSRKFVEAMAEYNEAQTLF
  RERSKGRIQRQLEITGRTTTDDELEEMLESGKPSIFTSDIISDSQITRQALNEIESRHKDIMKLETSIRE
  LHEMFMDMAMFVETQGEMINNIERNVMNATDYVEHAKEETKKAIKYQSKARRKKWIIIAVSVVLVAIIAL
  IIGLSVGK
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_194356
• ORF Size: 864 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_194356.1, NP_919337.1
• RefSeq Size: 3469 bp
• RefSeq ORF: 867 bp
• Locus ID: 2054
• Cytogenetics: 12q24.33
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: SNARE interactions in vesicular transport
• Gene Summary: 'The product of this gene belongs to the syntaxin/epimorphin family of proteins. The syntaxins are a large protein family implicated in the targeting and fusion of intracellular transport vesicles. The product of this gene regulates epithelial-mesenchymal interactions and epithelial cell morphogenesis and activation. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]'