PARP3 (NM_001003935) Human Tagged ORF Clone

CAT#: RG217717

PARP3 (GFP-tagged) - Human poly (ADP-ribose) polymerase family, member 3 (PARP3), transcript variant 3

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PARP3
• Synonyms: ADPRT3; ADPRTL2; ADPRTL3; hPARP-3; IRT1; pADPRT-3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG217717 representing NM_001003935
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCCAAAGCCGAAGCCCTGGGTACAGACTGAGGGCCCTGAGAAGAAGAAGGGCCGGCAGGCAGGAA
  GGGAGGAGGACCCCTTCCGCTCCACCGCTGAGGCCCTCAAGGCCATACCCGCAGAGAAGCGCATAATCCG
  CGTGGATCCAACATGTCCACTCAGCAGCAACCCCGGGACCCAGGTGTATGAGGACTACAACTGCACCCTG
  AACCAGACCAACATCGAGAACAACAACAACAAGTTCTACATCATCCAGCTGCTCCAAGACAGCAACCGCT
  TCTTCACCTGCTGGAACCGCTGGGGCCGTGTGGGAGAGGTCGGCCAGTCAAAGATCAACCACTTCACAAG
  GCTAGAAGATGCAAAGAAGGACTTTGAGAAGAAATTTCGGGAAAAGACCAAGAACAACTGGGCAGAGCGG
  GACCACTTTGTGTCTCACCCGGGCAAGTACACACTTATCGAAGTACAGGCAGAGGATGAGGCCCAGGAAG
  CTGTGGTGAAGGTGGACAGAGGCCCAGTGAGGACTGTGACTAAGCGGGTGCAGCCCTGCTCCCTGGACCC
  AGCCACGCAGAAGCTCATCACTAACATCTTCAGCAAGGAGATGTTCAAGAACACCATGGCCCTCATGGAC
  CTGGATGTGAAGAAGATGCCCCTGGGAAAGCTGAGCAAGCAACAGATTGCACGGGGTTTCGAGGCCTTGG
  AGGCGCTGGAGGAGGCCCTGAAAGGCCCCACGGATGGTGGCCAAAGCCTGGAGGAGCTGTCCTCACACTT
  TTACACCGTCATCCCGCACAACTTCGGCCACAGCCAGCCCCCGCCCATCAATTCCCCTGAGCTTCTGCAG
  GCCAAGAAGGACATGCTGCTGGTGCTGGCGGACATCGAGCTGGCCCAGGCCCTGCAGGCAGTCTCTGAGC
  AGGAGAAGACGGTGGAGGAGGTGCCACACCCCCTGGACCGAGACTACCAGCTTCTCAAGTGCCAGCTGCA
  GCTGCTAGACTCTGGAGCACCTGAGTACAAGGTGATACAGACCTACTTAGAACAGACTGGCAGCAACCAC
  AGGTGCCCTACACTTCAACACATCTGGAAAGTAAACCAAGAAGGGGAGGAAGACAGATTCCAGGCCCACT
  CCAAACTGGGTAATCGGAAGCTGCTGTGGCATGGCACCAACATGGCCGTGGTGGCCGCCATCCTCACTAG
  TGGGCTCCGCATCATGCCACATTCTGGTGGGCGTGTTGGCAAGGGCATCTACTTTGCCTCAGAGAACAGC
  AAGTCAGCTGGATATGTTATTGGCATGAAGTGTGGGGCCCACCATGTCGGCTACATGTTCCTGGGTGAGG
  TGGCCCTGGGCAGAGAGCACCATATCAACACGGACAACCCCAGCTTGAAGAGCCCACCTCCTGGCTTCGA
  CAGTGTCATTGCCCGAGGCCACACCGAGCCTGATCCGACCCAGGACACTGAGTTGGAGCTGGATGGCCAG
  CAAGTGGTGGTGCCCCAGGGCCAGCCTGTGCCCTGCCCAGAGTTCAGCAGCTCCACATTCTCCCAGAGCG
  AGTACCTCATCTACCAGGAGAGCCAGTGTCGCCTGCGCTACCTGCTGGAGGTCCACCTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG217717 representing NM_001003935
  Red=Cloning site Green=Tags(s)
  
  MAPKPKPWVQTEGPEKKKGRQAGREEDPFRSTAEALKAIPAEKRIIRVDPTCPLSSNPGTQVYEDYNCTL
  NQTNIENNNNKFYIIQLLQDSNRFFTCWNRWGRVGEVGQSKINHFTRLEDAKKDFEKKFREKTKNNWAER
  DHFVSHPGKYTLIEVQAEDEAQEAVVKVDRGPVRTVTKRVQPCSLDPATQKLITNIFSKEMFKNTMALMD
  LDVKKMPLGKLSKQQIARGFEALEALEEALKGPTDGGQSLEELSSHFYTVIPHNFGHSQPPPINSPELLQ
  AKKDMLLVLADIELAQALQAVSEQEKTVEEVPHPLDRDYQLLKCQLQLLDSGAPEYKVIQTYLEQTGSNH
  RCPTLQHIWKVNQEGEEDRFQAHSKLGNRKLLWHGTNMAVVAAILTSGLRIMPHSGGRVGKGIYFASENS
  KSAGYVIGMKCGAHHVGYMFLGEVALGREHHINTDNPSLKSPPPGFDSVIARGHTEPDPTQDTELELDGQ
  QVVVPQGQPVPCPEFSSSTFSQSEYLIYQESQCRLRYLLEVHL
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001003935
• ORF Size: 1599 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001003935.2, NP_001003935.2
• RefSeq Size: 2477
• RefSeq ORF: 1601
• Locus ID: 10039
• Protein Families: Druggable Genome
• Protein Pathways: Base excision repair
• Gene Summary: The protein encoded by this gene belongs to the PARP family. These enzymes modify nuclear proteins by poly-ADP-ribosylation, which is required for DNA repair, regulation of apoptosis, and maintenance of genomic stability. This gene encodes the poly(ADP-ribosyl)transferase 3, which is preferentially localized to the daughter centriole throughout the cell cycle. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]