CHD2 (NM_001042572) Human Tagged ORF Clone

CAT#: RG217803

CHD2 (GFP-tagged) - Human chromodomain helicase DNA binding protein 2 (CHD2), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: CHD2
• Synonyms: EEOC
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG217803 representing NM_001042572
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGAGAAATAAGGACAAAAGCCAAGAGGAGGACAGTTCGCTACACAGCAATGCATCGAGTCACTCAG
  CCTCTGAAGAAGCTTCGGGTTCAGACTCAGGCAGTCAGTCGGAAAGTGAGCAGGGAAGTGATCCAGGAAG
  TGGACATGGCAGCGAGTCGAACAGCAGCTCTGAATCTTCTGAGAGTCAGTCGGAATCTGAGAGCGAATCA
  GCAGGTTCCAAATCCCAGCCAGTCCTCCCAGAAGCCAAAGAGAAGCCAGCCTCTAAGAAGGAACGGATAG
  CTGATGTGAAGAAGATGTGGGAAGAATATCCTGATGTTTATGGGGTCAGGCGGTCAAACCGAAGCAGACA
  AGAACCATCGCGATTTAATATTAAGGAAGAGGCAAGTAGCGGGTCTGAGAGTGGGAGCCCAAAAAGAAGA
  GGCCAGAGGCAGCTGAAAAAACAAGAAAAATGGAAACAGGAACCCTCAGAAGATGAACAGGAACAAGGCA
  CCAGTGCAGAGAGTGAGCCAGAACAAAAAAAAGTAAAAGCCAGAAGACCTGTCCCCAGAAGAACAGTGCC
  CAAACCTCGTGTTAAAAAGCAGCCGAAGACTCAGCGTGGAAAGAGAAAAAAGCAAGATTCTTCTGATGAG
  GATGATGATGATGACGAAGCTCCCAAAAGGCAGACTCGTCGAAGAGCGGCTAAAAACGTTAGTTACAAAG
  AAGATGATGACTTTGAGACTGACTCAGATGATCTCATTGAAATGACTGGAGAAGGAGTTGATGAACAGCA
  AGATAATAGTGAAACTATTGAAAAGGTCTTAGATTCAAGACTGGGAAAGAAAGGAGCCACTGGAGCATCT
  ACTACTGTATATGCGATTGAAGCTAATGGCGACCCTAGTGGTGACTTTGACACTGAAAAGGATGAAGGTG
  AAATCCAGTACCTCATCAAGTGGAAGGGTTGGTCTTACATCCACAGCACATGGGAGAGTGAAGAATCCTT
  ACAGCAACAGAAAGTGAAGGGCCTAAAAAAACTAGAGAACTTCAAGAAAAAAGAGGACGAAATCAAACAA
  TGGTTAGGGAAAGTTTCTCCTGAAGATGTAGAATATTTCAATTGCCAACAGGAGCTGGCTTCAGAGTTGA
  ATAAACAGTATCAGATAGTAGAAAGAGTAATAGCTGTGAAGACAAGTAAATCTACATTGGGTCAAACAGA
  TTTTCCAGCTCATAGTCGGAAGCCGGCACCCTCAAATGAGCCCGAATATCTATGTAAATGGATGGGACTC
  CCCTATTCAGAGTGTAGCTGGGAAGATGAAGCCCTCATTGGAAAGAAATTCCAGAATTGCATTGACAGCT
  TCCACAGTAGGAACAACTCAAAAACCATCCCAACAAGAGAATGCAAGGCCCTGAAGCAGAGACCACGATT
  TGTAGCTTTAAAGAAACAACCTGCATATTTAGGAGGGGAGAATCTGGAACTTCGAGATTATCAGCTAGAA
  GGTCTAAACTGGCTAGCTCATTCCTGGTGCAAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG217803 representing NM_001042572
  Red=Cloning site Green=Tags(s)
  
  MMRNKDKSQEEDSSLHSNASSHSASEEASGSDSGSQSESEQGSDPGSGHGSESNSSSESSESQSESESES
  AGSKSQPVLPEAKEKPASKKERIADVKKMWEEYPDVYGVRRSNRSRQEPSRFNIKEEASSGSESGSPKRR
  GQRQLKKQEKWKQEPSEDEQEQGTSAESEPEQKKVKARRPVPRRTVPKPRVKKQPKTQRGKRKKQDSSDE
  DDDDDEAPKRQTRRRAAKNVSYKEDDDFETDSDDLIEMTGEGVDEQQDNSETIEKVLDSRLGKKGATGAS
  TTVYAIEANGDPSGDFDTEKDEGEIQYLIKWKGWSYIHSTWESEESLQQQKVKGLKKLENFKKKEDEIKQ
  WLGKVSPEDVEYFNCQQELASELNKQYQIVERVIAVKTSKSTLGQTDFPAHSRKPAPSNEPEYLCKWMGL
  PYSECSWEDEALIGKKFQNCIDSFHSRNNSKTIPTRECKALKQRPRFVALKKQPAYLGGENLELRDYQLE
  GLNWLAHSWCK
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001042572
• ORF Size: 1503 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001042572.1, NP_001036037.1
• RefSeq Size: 2395 bp
• RefSeq ORF: 1506 bp
• Locus ID: 1106
• Cytogenetics: 15q26.1
• Protein Families: Transcription Factors
• Gene Summary: 'The CHD family of proteins is characterized by the presence of chromo (chromatin organization modifier) domains and SNF2-related helicase/ATPase domains. CHD genes alter gene expression possibly by modification of chromatin structure thus altering access of the transcriptional apparatus to its chromosomal DNA template. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]'