STON1 (NM_006873) Human Tagged ORF Clone

CAT#: RG217975

STON1 (GFP-tagged) - Human stonin 1 (STON1), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: STON1
• Synonyms: SALF; SBLF; STN1; STNB1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG217975 representing NM_006873
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGCTCCACAAATCCAGGCAAATGGGTCACCTTTGATGATGATCCTGCTGTTCAATCTTCTCAAAAGT
  CAAAGAATTTTCCTCTGGAGAATCAAGGTGTCTGTAGACCAAATGGACTGAAGCTGAACCTTCCTGGCCT
  CAGGGAATTTCCCAGTGGATCTTCCTCCACCAGCAGCACTCCTCTCTCCTCCCCCATTGTAGATTTTTAT
  TTCAGTCCAGGACCTCCAAGTAACTCTCCTCTTTCTACACCTACCAAAGACTTCCCAGGTTTTCCTGGCA
  TCCCCAAAGCAGGGACTCATGTGCTTTATCCTATTCCAGAATCATCTTCAGACAGCCCACTCGCAATATC
  AGGAGGAGAATCTTCCTTACTGCCTACCAGACCAACATGTTTATCCCATGCCTTGTTACCCAGTGACCAC
  TCATGTACACATCCAACTCCCAAAGTAGGTCTTCCAGATGAAGTTAATCCTCAACAGGCTGAAAGCCTAG
  GATTCCAAAGTGATGATCTCCCCCAGTTTCAGTATTTTCGAGAGGACTGTGCTTTTTCAAGTCCATTTTG
  GAAAGATGAAGGCAGTGATTCCCATTTCACCCTTGACCCACCAGGAAGCAAAAAGATGTTCTCATCAAGA
  AACAAGGAGATGCCTATTGACCAAAAAAGCCTAAATAAGTGTTCACTCAACTATATCTGTGAGAAGCTTG
  AACATCTCCAGTCAGCTGAGAACCAAGACTCACTTAGAAGTTTGTCTATGCACTGTCTATGTGCTGAAGA
  AAATGCCTCTTCCTTTGTCCCCCACACACTCTTCAGGAGTCAGCCAAAATCCGGATGGTCTTTCATGCTG
  AGAATTCCTGAGAAGAAGAATATGATGTCTTCCCGGCAATGGGGACCAATTTTTCTGAAAGTTTTGCCTG
  GAGGAATTTTGCAGATGTATTATGAACAGGGATTAGAAAAACCATTTAAAGAGATACAGCTTGATCCATA
  TTGTAGGCTTTCTGAACCCAAGGTTGAGAACTTCAGTGTAGCAGGAAAAATCCACACTGTGAAGATTGAA
  CATGTGTCTTACACAGAAAAAAGGAAATACCATTCTAAGACAGAAGTAGTTCATGAACCTGACATAGAGC
  AGATGCTGAAGTTGGGGTCCACATCGTACCATGACTTCCTTGACTTTCTGACTACTGTGGAGGAGGAGCT
  GATGAAGTTGCCAGCTGTTTCAAAACCAAAAAAGAACTACGAGGAGCAAGAAATTTCCTTGGAAATTGTG
  GACAACTTTTGGGGTAAAGTCACAAAAGAAGGAAAATTTGTTGAAAGTGCTGTGATAACTCAAATGTATT
  GCCTCTGCTTTGTGAATGGGAACCTGGAATGCTTTTTAACCTTGAATGACCTTGAGTTGCCGAAGCGAGA
  TGAATCCTATTATGAGAAGGACTCAGAAAAAAAGGGGATTGATATTCTTGACTACCATTTTCATAAGTGT
  GTGAATGTACAAGAATTTGAGCAATCAAGAATCATTAAGTTTGTACCTCTGGATGCCTGCCGGTTTGAGC
  TGATGCGTTTCAAGACTTTGTATAATGGGGATAATCTTCCCTTTTCCTTGAAGTCTGTAGTGGTTGTCCA
  GGGAGCATACGTGGAACTTCAGGCTTTTGTCAACATGGCCTCATTGGCGCAGAGGTCATCCTATGCTGGT
  TCCTTAAGGTCCTGTGACAATATAAGGATACACTTTCCTGTCCCATCGCAGTGGATCAAGGCCCTTTGGA
  CCATGAACCTCCAGAGGCAGAAGTCTCTGAAAGCTAAAATGAACCGCCGAGCATGTCTGGGGAGTTTACA
  GGAACTTGAATCTGAACCTGTCATTCAAGTCACTGTGGGGTCAGCAAAATATGAGAGTGCCTACCAGGCA
  GTGGTATGGAAGATAGATCGGCTTCCAGACAAAAATTCAAGTCTAGATCATCCCCATTGTCTGTCATACA
  AATTAGAGCTTGGATCAGACCAAGAAATTCCCTCTGATTGGTATCCATTTGCTACTGTTCAGTTTTCCGT
  GCCTGACACCTGTGCCTCAAGGACAGAGGTCAGGTCTCTGGGAGTGGAGAGTGATGTCCAGCCACAGAAA
  CATGTTCAGCAGCGAGCTTGCTACAACATCCAGGTTGAAATAGAAAAAAAGTGGATTAAAATCGATGGAG
  AAGACCCAGATAAAATTGGTGACTGCATAACTCAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG217975 representing NM_006873
  Red=Cloning site Green=Tags(s)
  
  MCSTNPGKWVTFDDDPAVQSSQKSKNFPLENQGVCRPNGLKLNLPGLREFPSGSSSTSSTPLSSPIVDFY
  FSPGPPSNSPLSTPTKDFPGFPGIPKAGTHVLYPIPESSSDSPLAISGGESSLLPTRPTCLSHALLPSDH
  SCTHPTPKVGLPDEVNPQQAESLGFQSDDLPQFQYFREDCAFSSPFWKDEGSDSHFTLDPPGSKKMFSSR
  NKEMPIDQKSLNKCSLNYICEKLEHLQSAENQDSLRSLSMHCLCAEENASSFVPHTLFRSQPKSGWSFML
  RIPEKKNMMSSRQWGPIFLKVLPGGILQMYYEQGLEKPFKEIQLDPYCRLSEPKVENFSVAGKIHTVKIE
  HVSYTEKRKYHSKTEVVHEPDIEQMLKLGSTSYHDFLDFLTTVEEELMKLPAVSKPKKNYEEQEISLEIV
  DNFWGKVTKEGKFVESAVITQMYCLCFVNGNLECFLTLNDLELPKRDESYYEKDSEKKGIDILDYHFHKC
  VNVQEFEQSRIIKFVPLDACRFELMRFKTLYNGDNLPFSLKSVVVVQGAYVELQAFVNMASLAQRSSYAG
  SLRSCDNIRIHFPVPSQWIKALWTMNLQRQKSLKAKMNRRACLGSLQELESEPVIQVTVGSAKYESAYQA
  VVWKIDRLPDKNSSLDHPHCLSYKLELGSDQEIPSDWYPFATVQFSVPDTCASRTEVRSLGVESDVQPQK
  HVQQRACYNIQVEIEKKWIKIDGEDPDKIGDCITQ
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006873
• ORF Size: 2205 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_006873.2, NP_006864.2
• RefSeq Size: 5822
• RefSeq ORF: 2208
• Locus ID: 11037
• Domains: Adap_comp_sub
• Protein Families: Transcription Factors
• Protein Pathways: Basal transcription factors
• Gene Summary: Endocytosis of cell surface proteins is mediated by a complex molecular machinery that assembles on the inner surface of the plasma membrane. This gene encodes one of two human homologs of the Drosophila melanogaster stoned B protein. This protein is related to components of the endocytic machinery and exhibits a modular structure consisting of an N-terminal proline-rich domain, a central region of homology specific to the human stoned B-like proteins, and a C-terminal region homologous to the mu subunits of adaptor protein (AP) complexes. Read-through transcription of this gene into the neighboring downstream gene, which encodes TFIIA-alpha/beta-like factor, generates a transcript (SALF), which encodes a fusion protein comprised of sequence sharing identity with each individual gene product. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2010]