RAP1GDS1 (NM_001100430) Human Tagged ORF Clone

CAT#: RG217996

RAP1GDS1 (GFP-tagged) - Human RAP1, GTP-GDP dissociation stimulator 1 (RAP1GDS1), transcript variant 6

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: RAP1GDS1
• Synonyms: GDS1; SmgGDS
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG217996 representing NM_001100430
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGATAATCTCAGTGATACCTTGAAGAAGCTGAAGATAACAGCTGTTGACAAGACTGAGGATAGTT
  TAGAAGGATGCTTGGATTGTCTGCTTCAAGCCCTGGCTCAAAATAATACGGAAACAAGTGAAAAAATCCA
  AGCAAGTGGAATACTTCAGCTGTTTGCAAGTCTGTTGACTCCACAGTCTTCCTGCAAAGCCAAAGTAGCT
  AACATCATAGCAGAAGTAGCCAAAAATGAGTTTATGCGAATTCCATGTGTGGATGCTGGATTGATTTCAC
  CACTGGTGCAGCTGCTAAATAGCAAAGACCAGGAAGTGCTGCTTCAAACGGGCAGGGCTCTAGGAAACAT
  ATGTTACGATAGCCAGTCAAGTAAAGAACAGTTTGCCAGTACAAACATTGCTGAAGAGCTAGTAAAACTC
  TTCAAGAAACAAATAGAACATGATAAGAGAGAAATGATTTTTGAAGTTCTTGCTCCATTGGCAGAAAATG
  ATGCTATTAAACTACAGCTGGTTGAAGCAGGCCTAGTAGAGTGTCTACTAGAGATTGTTCAGCAAAAAGT
  GGATAGTGACAAAGAAGATGATATTACTGAGCTCAAAACTGGTTCAGATCTCATGGTTTTATTACTTCTT
  GGAGATGAATCCATGCAGAAGTTATTTGAAGGAGGAAAAGGTAGTGTATTTCAAAGGGTACTCTCTTGGA
  TCCCATCAAATAACCACCAGCTACAGCTTGCTGGAGCATTGGCAATTGCAAATTTTGCCAGAAATGATGC
  AAATTGTATTCATATGGTAGACAATGGGATTGTAGAAAAACTTATGGATTTACTGGACAGACATGTAGAA
  GATGGAAATGTAACAGTACAGCATGCAGCACTAAGTGCCCTCAGAAACCTGGCCATTCCAGTTATAAATA
  AAGCAAAGATGTTATCAGCTGGGGTCACAGAGGCAGTTTTGAAATTTCTTAAATCTGAAATGCCTCCTGT
  TCAGTTCAAACTTCTGGGAACATTAAGAATGTTAATAGATGCACAAGCAGAAGCTGCTGAACAATTGGGA
  AAGAATGTTAAGTTAGTGGAGCGTTTGGTGGAATGGTGTGAAGCCAAAGATCATGCTGGTGTGATGGGGG
  AGTCAAACAGACTGCTGTCTGCCCTTATACGACACAGTAAATCAAAAGATGTAATTAAAACCATTGTGCA
  GAGTGGTGGCATCAAGCATCTAGTTACCATGGCAACTAGTGAACATGTAATAATGCAGAATGAAGCTCTT
  GTTGCTTTGGCATTAATAGCAGCTTTAGAATTGGGCACTGCTGAGAAAGATCTAGAAAGTGCTAAACTTG
  TACAGATTTTACATAGACTGCTAGCAGATGAGAGAAGTGCTCCTGAAATCAAATATAATTCCATGGTCCT
  GATATGTGCTCTTATGGGATCTGAATGTCTACACAAGGAAGTACAGGATTTGGCTTTTCTAGATGTCGTA
  TCCAAACTTCGCAGTCATGAGAACAAAAGTGTTGCCCAGCAGGCCTCTCTCACAGAGCAGAGACTTACTG
  TGGAAAGC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG217996 representing NM_001100430
  Red=Cloning site Green=Tags(s)
  
  MADNLSDTLKKLKITAVDKTEDSLEGCLDCLLQALAQNNTETSEKIQASGILQLFASLLTPQSSCKAKVA
  NIIAEVAKNEFMRIPCVDAGLISPLVQLLNSKDQEVLLQTGRALGNICYDSQSSKEQFASTNIAEELVKL
  FKKQIEHDKREMIFEVLAPLAENDAIKLQLVEAGLVECLLEIVQQKVDSDKEDDITELKTGSDLMVLLLL
  GDESMQKLFEGGKGSVFQRVLSWIPSNNHQLQLAGALAIANFARNDANCIHMVDNGIVEKLMDLLDRHVE
  DGNVTVQHAALSALRNLAIPVINKAKMLSAGVTEAVLKFLKSEMPPVQFKLLGTLRMLIDAQAEAAEQLG
  KNVKLVERLVEWCEAKDHAGVMGESNRLLSALIRHSKSKDVIKTIVQSGGIKHLVTMATSEHVIMQNEAL
  VALALIAALELGTAEKDLESAKLVQILHRLLADERSAPEIKYNSMVLICALMGSECLHKEVQDLAFLDVV
  SKLRSHENKSVAQQASLTEQRLTVES
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001100430
• ORF Size: 1548 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001100430.1, NP_001093900.1
• RefSeq Size: 3494 bp
• RefSeq ORF: 1551 bp
• Locus ID: 5910
• Cytogenetics: 4q23
• Gene Summary: 'The smg GDP dissociation stimulator (smgGDS) protein is a stimulatory GDP/GTP exchange protein with GTPase activity (Riess et al., 1993 [PubMed 8262526]).[supplied by OMIM, Feb 2010]'