NPFFR2 (NM_004885) Human Tagged ORF Clone

CAT#: RG218229

NPFFR2 (GFP-tagged) - Human neuropeptide FF receptor 2 (NPFFR2), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: NPFFR2
• Synonyms: GPR74; HLWAR77; NPFF2; NPGPR
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG218229 representing NM_004885
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATAGCTTCTTCGGAACCCCAGCGGCCAGCTGGTGCCTCCTGGAAAGTGACGTCTCATCTGCACCGG
  ACAAGGAGGCGGGGAGGGAGCGCAGAGCACTCAGCGTCCAGCAGCGCGGCGGGCCAGCCTGGAGCGGAAG
  CCTGGAGTGGAGCAGGCAGTCCGCGGGGGACAGACGTCGGCTGGGATTGAGCCGGCAGACTGCGAAAAGT
  AGCTGGAGCCGGAGCAGGGACAGAACCTGTTGCTGCAGACGGGCTTGGTGGATTCTGGTTCCTGCCGCCG
  ACAGGGCTCGCCGGGAGAGGTTCATCATGAATGAGAAATGGGACACAAACTCTTCAGAAAACTGGCATCC
  CATCTGGAATGTCAATGACACAAAGCATCATCTGTACTCAGATATTAATATTACCTATGTGAACTACTAT
  CTTCACCAGCCTCAAGTGGCAGCAATCTTCATTATTTCCTACTTTCTGATCTTCTTTTTGTGCATGATGG
  GAAATACTGTGGTTTGCTTTATTGTAATGAGGAACAAACATATGCACACAGTCACTAATCTCTTCATCTT
  AAACCTGGCCATAAGTGATTTACTAGTTGGCATATTCTGCATGCCTATAACACTGCTGGACAATATTATA
  GCAGGATGGCCATTTGGAAACACGATGTGCAAGATCAGTGGATTGGTCCAGGGAATATCTGTCGCAGCTT
  CAGTCTTTACGTTAGTTGCAATTGCTGTAGATAGGTTCCAGTGTGTGGTCTACCCTTTTAAACCAAAGCT
  CACTATCAAGACAGCGTTTGTCATTATTATGATCATCTGGGTCCTAGCCATCACCATTATGTCTCCATCT
  GCAGTAATGTTACATGTGCAAGAAGAAAAATATTACCGAGTGAGACTCAACTCCCAGAATAAAACCAGTC
  CAGTCTACTGGTGCCGGGAAGACTGGCCAAATCAGGAAATGAGGAAGATCTACACCACTGTGCTGTTTGC
  CAACATCTACCTGGCTCCCCTCTCCCTCATTGTCATCATGTATGGAAGGATTGGAATTTCACTCTTCAGG
  GCTGCAGTTCCTCACACAGGCAGGAAGAACCAGGAGCAGTGGCACGTGGTGTCCAGGAAAAAGCAGAAGA
  TCATTAAGATGCTCCTGATTGTGGCCCTGCTTTTTATTCTCTCATGGCTGCCCCTGTGGACTCTAATGAT
  GCTCTCAGACTACGCTGACCTTTCTCCAAATGAACTGCAGATCATCAACATCTACATCTACCCTTTTGCA
  CACTGGCTGGCATTCGGCAACAGCAGTGTCAATCCCATCATTTATGGTTTCTTCAACGAGAATTTCCGCC
  GTGGTTTCCAAGAAGCTTTCCAGCTCCAGCTCTGCCAAAAAAGAGCAAAGCCTATGGAAGCTTATACCCT
  AAAAGCTAAAAGCCATGTGCTCATAAACACATCTAATCAGCTTGTCCAGGAATCTACATTTCAAAACCCT
  CATGGGGAAACCTTGCTTTATAGGAAAAGTGCTGAAAAACCCCAACAGGAATTAGTGATGGAAGAATTAA
  AAGAAACTACTAACAGCAGTGAGATT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG218229 representing NM_004885
  Red=Cloning site Green=Tags(s)
  
  MNSFFGTPAASWCLLESDVSSAPDKEAGRERRALSVQQRGGPAWSGSLEWSRQSAGDRRRLGLSRQTAKS
  SWSRSRDRTCCCRRAWWILVPAADRARRERFIMNEKWDTNSSENWHPIWNVNDTKHHLYSDINITYVNYY
  LHQPQVAAIFIISYFLIFFLCMMGNTVVCFIVMRNKHMHTVTNLFILNLAISDLLVGIFCMPITLLDNII
  AGWPFGNTMCKISGLVQGISVAASVFTLVAIAVDRFQCVVYPFKPKLTIKTAFVIIMIIWVLAITIMSPS
  AVMLHVQEEKYYRVRLNSQNKTSPVYWCREDWPNQEMRKIYTTVLFANIYLAPLSLIVIMYGRIGISLFR
  AAVPHTGRKNQEQWHVVSRKKQKIIKMLLIVALLFILSWLPLWTLMMLSDYADLSPNELQIINIYIYPFA
  HWLAFGNSSVNPIIYGFFNENFRRGFQEAFQLQLCQKRAKPMEAYTLKAKSHVLINTSNQLVQESTFQNP
  HGETLLYRKSAEKPQQELVMEELKETTNSSEI
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004885
• ORF Size: 1566 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_004885.1, NP_004876.1
• RefSeq Size: 1936
• RefSeq ORF: 1569
• Locus ID: 10886
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Neuroactive ligand-receptor interaction
• Gene Summary: This gene encodes a member of a subfamily of G-protein-coupled neuropeptide receptors. This protein is activated by the neuropeptides A-18-amide (NPAF) and F-8-amide (NPFF) and may function in pain modulation and regulation of the opioid system. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2009]