RNF6 (NM_183043) Human Tagged ORF Clone

CAT#: RG218744

RNF6 (GFP-tagged) - Human ring finger protein (C3H2C3 type) 6 (RNF6), transcript variant 4

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: RNF6
• Synonyms: DKFZp686P0776
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG218744 representing NM_183043
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATCAGTCTAGATCGAGATCAGATGGTGGCAGTGAAGAAACCTTACCTCAAGACCATAATCATCATG
  AAAATGAGAGAAGATGGCAGCAAGAGCGTCTCCACAGAGAAGAGGCCTATTATCAGTTTATTAATGAACT
  CAATGATGAAGATTATCGGCTTATGAGAGACCATAATCTTTTAGGCACCCCTGGAGAAATAACATCAGAA
  GAACTGCAACAGCGGTTAGATGGCGTCAAGGAACAACTAGCATCTCAGCCTGACTTGAGAGATGGAACGA
  ATTACAGAGACTCAGAAGTCCCTAGAGAAAGTTCACATGAAGATTCTCTTCTAGAATGGTTGAACACCTT
  TCGGCGCACAGGAAATGCAACTCGAAGTGGACAAAATGGGAACCAAACTTGGAGAGCTGTGAGTCGAACA
  AACCCGAACAATGGAGAGTTTCGGTTTAGTTTGGAAATCCACGTAAATCATGAAAATAGAGGATTTGAAA
  TTCATGGAGAAGATTATACAGACATTCCACTTTCAGATAGTAACAGAGATCATACTGCAAATAGGCAACA
  AAGGTCAACTAGTCCTGTGGCTAGGCGAACAAGAAGCCAAACCTCAGTGAATTTCAATGGTAGTAGTTCC
  AACATTCCAAGGACTAGGCTTGCTTCAAGGGGGCAAAATCCAGCTGAAGGATCTTTCTCAACATTGGGAA
  GGTTAAGAAATGGAATTGGGGGAGCAGCTGGCATTCCTCGAGCTAACGCTTCACGCACTAATTTCAGTAG
  TCACACAAACCAATCAGGTGGTAGTGAACTCAGGCAAAGGGAGGGGCAACGGTTTGGAGCAGCACATGTT
  TGGGAAAATGGGGCTAGAAGTAATGTTACAGTGAGGAATACAAACCAAAGATTAGAGCCAATAAGATTAC
  GATCTACTTCCAATAGTCGAAGCCGTTCACCAATTCAGAGACAGAGTGGCACTGTTTATCATAATTCCCA
  AAGGGAAAGTAGACCAGTACAGCAAACCACTAGAAGATCTGTTAGGAGGAGAGGTAGAACTCGAGTCTTT
  TTAGAGCAAGATAGAGAACGAGAACGCAGAGGTACTGCATATACCCCATTCTCTAATTCAAGGCTTGTGT
  CAAGAATAACAGTAGAAGAAGGAGAAGAATCCAGCAGATCCTCAACTGCTGTACGACGACATCCAACAAT
  CACACTGGACCTTCAAGTGAGAAGGATCCGTCCTGGAGAAAATAGAGATCGGGATAGTATTGCAAATAGA
  ACTCGATCCAGAGTAGGGCTAGCAGAAAATACAGTCACTATTGAAAGCAATAGTGGGGGCTTTCGCCGAA
  CCATTTCTCGTTTAGAGCGGTCAGGTATTCGAACCTATGTTAGTACCATAACAGTTCCTCTTCGTAGGAT
  TTCTGAGAATGAGCTTGTTGAGCCATCATCAGTGGCTCTTCGGTCAATTTTAAGGCAGATCATGACTGGG
  TTTGGAGAACTGAGTTCTCTAATGGAGGCCGATTCTGAGTCAGAACTTCAAAGAAATGGCCAGCATTTAC
  CAGACATGCACTCAGAACTGAGTAACTTAGGTACAGATAACAACAGGAGCCAGCACAGGGAAGGTTCCTC
  TCAAGACAGGCAGGCCCAAGGAGACAGCACTGAAATGCATGGTGAAAACGAGACCACCCAGCCTCATACT
  CGAAACAGTGACAGTAGGGGTGGCAGGCAGTTGCGAAATCCAAACAATTTAGTTGAAACTGGAACACTAC
  CCATTCTTCGCCTTGCTCACTTTTTTTTACTAAATGAAAGTGATGATGATGATCGAATACGTGGTTTAAC
  CAAAGAGCAGATTGACAATCTTTCCACCAGGCACTATGAGCATAACAGTATTGATAGTGAACTAGGTAAA
  ATCTGTAGTGTTTGTATTAGTGACTATGTAACTGGAAACAAGCTCAGGCAATTACCTTGCATGCATGAAT
  TTCACATTCATTGTATTGACCGATGGCTCTCAGAGAATTGCACTTGTCCGATCTGTCGGCAGCCTGTTTT
  AGGGTCTAACATAGCAAACAATGGG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG218744 representing NM_183043
  Red=Cloning site Green=Tags(s)
  
  MNQSRSRSDGGSEETLPQDHNHHENERRWQQERLHREEAYYQFINELNDEDYRLMRDHNLLGTPGEITSE
  ELQQRLDGVKEQLASQPDLRDGTNYRDSEVPRESSHEDSLLEWLNTFRRTGNATRSGQNGNQTWRAVSRT
  NPNNGEFRFSLEIHVNHENRGFEIHGEDYTDIPLSDSNRDHTANRQQRSTSPVARRTRSQTSVNFNGSSS
  NIPRTRLASRGQNPAEGSFSTLGRLRNGIGGAAGIPRANASRTNFSSHTNQSGGSELRQREGQRFGAAHV
  WENGARSNVTVRNTNQRLEPIRLRSTSNSRSRSPIQRQSGTVYHNSQRESRPVQQTTRRSVRRRGRTRVF
  LEQDRERERRGTAYTPFSNSRLVSRITVEEGEESSRSSTAVRRHPTITLDLQVRRIRPGENRDRDSIANR
  TRSRVGLAENTVTIESNSGGFRRTISRLERSGIRTYVSTITVPLRRISENELVEPSSVALRSILRQIMTG
  FGELSSLMEADSESELQRNGQHLPDMHSELSNLGTDNNRSQHREGSSQDRQAQGDSTEMHGENETTQPHT
  RNSDSRGGRQLRNPNNLVETGTLPILRLAHFFLLNESDDDDRIRGLTKEQIDNLSTRHYEHNSIDSELGK
  ICSVCISDYVTGNKLRQLPCMHEFHIHCIDRWLSENCTCPICRQPVLGSNIANNG
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_183043
• ORF Size: 2055 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_183043.1, NP_898864.1
• RefSeq Size: 3284 bp
• RefSeq ORF: 2058 bp
• Locus ID: 6049
• Cytogenetics: 13q12.13
• Protein Families: Druggable Genome
• Gene Summary: 'The protein encoded by this gene contains a RING-H2 finger motif. Deletions and mutations in this gene were detected in esophageal squamous cell carcinoma (ESCC), suggesting that this protein may be a potential tumor suppressor. Studies of the mouse counterpart suggested a role of this protein in the transcription regulation that controls germinal differentiation. Multiple alternatively spliced transcript variants encoding the same protein are observed. [provided by RefSeq, Jul 2008]'