Transportin 1 (TNPO1) (NM_002270) Human Tagged ORF Clone

CAT#: RG218884

TNPO1 (GFP-tagged) - Human transportin 1 (TNPO1), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: TNPO1
• Synonyms: IPO2; KPNB2; MIP; MIP1; TRN
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG218884 representing NM_002270
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGTATGAGTGGAAACCTGACGAGCAAGGGCTTCAGCAAATCCTGCAGCTGTTGAAGGAGTCCCAGT
  CCCCAGACACCACCATCCAGAGAACCGTGCAACAAAAACTGGAACAACTTAATCAGTATCCAGACTTTAA
  CAACTACTTGATTTTTGTTCTTACAAAATTAAAATCTGAAGATGAACCCACAAGATCATTGAGTGGTCTT
  ATCTTGAAGAATAATGTGAAAGCACACTTTCAGAACTTCCCAAATGGTGTAACAGACTTTATTAAAAGTG
  AATGTTTAAATAATATTGGTGACTCCTCTCCTCTGATTAGAGCCACTGTTGGTATTTTGATCACAACTAT
  AGCCTCCAAGGGAGAATTGCAGAATTGGCCTGACCTCTTACCAAAACTCTGTAGCCTGTTGGATTCTGAA
  GATTATAATACCTGTGAGGGAGCATTTGGTGCCCTTCAGAAGATTTGTGAAGATTCTGCTGAGATTTTAG
  ACAGTGATGTTTTAGATCGTCCTCTCAACATCATGATTCCCAAATTTTTACAGTTCTTCAAGCATAGTAG
  TCCAAAAATAAGGTCTCACGCTGTTGCATGTGTCAATCAGTTTATCATCAGTAGGACTCAAGCTCTAATG
  TTGCACATTGATTCTTTTATTGAGAATCTCTTTGCATTAGCTGGTGATGAAGAACCAGAGGTACGGAAAA
  ATGTGTGCCGAGCACTTGTGATGTTGCTCGAAGTTCGAATGGATCGCCTGCTTCCTCACATGCATAATAT
  AGTTGAGTACATGCTACAGAGGACTCAAGATCAAGATGAAAATGTGGCTTTAGAAGCCTGTGAATTTTGG
  CTAACTTTAGCTGAACAGCCAATATGCAAAGATGTACTCGTAAGGCATCTTCCTAAGTTGATTCCTGTGT
  TAGTGAATGGCATGAAGTACTCAGACATAGATATTATCCTACTTAAGGGTGATGTTGAAGAAGACGAAAC
  GATTCCTGATAGTGAACAGGATATACGGCCACGTTTTCACCGATCGAGGACGGTGGCTCAGCAGCATGAT
  GAAGATGGAATTGAAGAGGAAGATGATGATGATGATGAAATTGATGATGATGATACAATTTCTGACTGGA
  ATCTAAGAAAATGTTCTGCTGCTGCCCTGGATGTTCTTGCAAATGTGTATCGTGATGAACTGCTGCCACA
  TATTTTGCCCCTTTTGAAAGAATTACTTTTTCATCATGAATGGGTTGTTAAAGAATCAGGCATTTTGGTT
  TTAGGAGCAATTGCTGAAGGTTGCATGCAGGGCATGATTCCATACTTGCCTGAGCTTATTCCTCACCTTA
  TTCAGTGCCTCTCTGATAAAAAGGCTCTTGTGCGTTCCATAACATGCTGGACTCTTAGCCGCTATGCACA
  CTGGGTGGTCAGCCAGCCGCCAGACACGTACCTGAAGCCATTAATGACAGAATTGCTAAAGCGCATCCTG
  GATAGCAACAAGAGAGTACAAGAAGCTGCCTGCAGTGCCTTTGCTACCCTAGAAGAGGAGGCTTGTACAG
  AACTTGTTCCTTACCTTGCTTATATACTTGATACCCTGGTCTTTGCATTTAGTAAATACCAGCATAAGAA
  CCTGCTCATTCTTTACGATGCCATAGGAACATTAGCAGATTCAGTAGGACATCATTTAAACAAACCAGAA
  TATATTCAGATGCTAATGCCTCCACTGATCCAGAAATGGAACATGTTAAAGGATGAAGATAAAGATCTCT
  TCCCTTTACTTGAGTGCCTATCTTCAGTTGCCACAGCACTGCAGTCTGGATTCCTTCCGTACTGTGAACC
  TGTGTATCAGCGTTGTGTAAACCTAGTACAGAAGACTCTTGCACAAGCCATGCTAAACAATGCTCAACCA
  GATCAATATGAAGCTCCAGATAAAGATTTTATGATAGTGGCTCTTGATTTACTGAGTGGCCTGGCTGAAG
  GACTTGGAGGCAACATTGAACAGCTGGTAGCCCGAAGTAACATCCTGACACTAATGTATCAGTGCATGCA
  GGATAAAATGCCAGAAGTTCGACAGAGTTCTTTTGCCCTGTTAGGTGACCTCACAAAAGCTTGCTTTCAG
  CATGTTAAGCCTTGTATAGCTGATTTCATGCCAATATTGGGAACCAACCTAAATCCAGAATTCATTTCAG
  TCTGCAACAATGCCACATGGGCAATTGGAGAAATCTCCATTCAAATGGGTATAGAGATGCAGCCTTATAT
  TCCTATGGTGTTGCACCAGCTTGTAGAAATCATTAACAGACCCAACACACCAAAGACGTTGTTAGAGAAT
  ACAGCAATAACAATTGGTCGTCTTGGTTACGTTTGTCCTCAAGAGGTGGCCCCCATGCTACAGCAGTTTA
  TAAGACCCTGGTGCACCTCTCTGAGAAACATAAGAGACAATGAGGAAAAGGATTCAGCATTCCGTGGAAT
  TTGTACCATGATCAGTGTGAATCCCAGTGGCGTAATCCAAGATTTTATATTTTTTTGTGATGCCGTTGCA
  TCATGGATTAACCCAAAAGATGATCTCAGAGACATGTTCTGTAAGATCCTTCATGGATTTAAAAATCAAG
  TTGGCGATGAAAATTGGAGGCGTTTCTCTGACCAGTTTCCTCTTCCCTTAAAAGAGCGTCTTGCAGCTTT
  TTATGGTGTT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG218884 representing NM_002270
  Red=Cloning site Green=Tags(s)
  
  MEYEWKPDEQGLQQILQLLKESQSPDTTIQRTVQQKLEQLNQYPDFNNYLIFVLTKLKSEDEPTRSLSGL
  ILKNNVKAHFQNFPNGVTDFIKSECLNNIGDSSPLIRATVGILITTIASKGELQNWPDLLPKLCSLLDSE
  DYNTCEGAFGALQKICEDSAEILDSDVLDRPLNIMIPKFLQFFKHSSPKIRSHAVACVNQFIISRTQALM
  LHIDSFIENLFALAGDEEPEVRKNVCRALVMLLEVRMDRLLPHMHNIVEYMLQRTQDQDENVALEACEFW
  LTLAEQPICKDVLVRHLPKLIPVLVNGMKYSDIDIILLKGDVEEDETIPDSEQDIRPRFHRSRTVAQQHD
  EDGIEEEDDDDDEIDDDDTISDWNLRKCSAAALDVLANVYRDELLPHILPLLKELLFHHEWVVKESGILV
  LGAIAEGCMQGMIPYLPELIPHLIQCLSDKKALVRSITCWTLSRYAHWVVSQPPDTYLKPLMTELLKRIL
  DSNKRVQEAACSAFATLEEEACTELVPYLAYILDTLVFAFSKYQHKNLLILYDAIGTLADSVGHHLNKPE
  YIQMLMPPLIQKWNMLKDEDKDLFPLLECLSSVATALQSGFLPYCEPVYQRCVNLVQKTLAQAMLNNAQP
  DQYEAPDKDFMIVALDLLSGLAEGLGGNIEQLVARSNILTLMYQCMQDKMPEVRQSSFALLGDLTKACFQ
  HVKPCIADFMPILGTNLNPEFISVCNNATWAIGEISIQMGIEMQPYIPMVLHQLVEIINRPNTPKTLLEN
  TAITIGRLGYVCPQEVAPMLQQFIRPWCTSLRNIRDNEEKDSAFRGICTMISVNPSGVIQDFIFFCDAVA
  SWINPKDDLRDMFCKILHGFKNQVGDENWRRFSDQFPLPLKERLAAFYGV
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002270
• ORF Size: 2694 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_002270.3, NP_002261.3
• RefSeq Size: 8559
• RefSeq ORF: 2697
• Locus ID: 3842
• Domains: Armadillo_seg, IBN_NT
• Protein Families: Druggable Genome
• Gene Summary: This gene encodes the beta subunit of the karyopherin receptor complex which interacts with nuclear localization signals to target nuclear proteins to the nucleus. The karyopherin receptor complex is a heterodimer of an alpha subunit which recognizes the nuclear localization signal and a beta subunit which docks the complex at nucleoporins. Alternate splicing of this gene results in several transcript variants encoding different proteins. [provided by RefSeq, Jun 2018]