UBE3B (NM_130466) Human Tagged ORF Clone

CAT#: RG219024

UBE3B (GFP-tagged) - Human ubiquitin protein ligase E3B (UBE3B), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: UBE3B
• Synonyms: BPIDS; KOS
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG219024 representing NM_130466
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTCACCCTGTCTCAGACCTCGAGAGCATGGTTCATCGATAGAGCCCGTCAGGCACGAGAAGAAAGGC
  TTGTGCAGAAGGAACGGGAGCGGGCAGCTGTTGTGATCCAGGCCCATGTCCGGAGTTTTCTCTGTCGGAG
  TCGACTGCAGAGAGATATCAGGAGAGAGATTGATGACTTTTTTAAAGCAGATGACCCTGAGTCCACTAAA
  AGAAGTGCACTTTGTATTTTCAAGATTGCCAGGAAACTGCTGTTCCTATTCAGAATCAAAGAGGATAATG
  AGAGATTTGAGAAGTTGTGTCGCAGCATCCTGAGCAGCATGGATGCTGAGAATGAGCCTAAGGTGTGGTA
  TGTGTCCCTGGCTTGTTCTAAGGACCTCACCCTCCTTTGGATTCAACAGATCAAGAACATTTTGTGGTAC
  TGCTGTGATTTTCTCAAGCAGCTCAAGCCTGAAATCCTGCAGGACTCCCGACTCATCACCCTGTACCTCA
  CGATGCTTGTCACCTTCACAGACACTTCAACGTGGAAAATTCTTCGGGGAAAAGGTGAAAGTCTTCGACC
  AGCGATGAACCACATTTGTGCAAATATAATGGGACATCTCAACCAGCATGGATTTTATTCTGTGCTGCAG
  ATATTGTTAACCCGTGGCCTGGCAAGACCCCGTCCTTGTCTATCCAAAGGCACTTTAACAGCAGCTTTTT
  CTCTAGCGTTACGCCCTGTGATTGCTGCACAGTTCTCAGACAATCTGATTCGGCCGTTCCTCATCCACAT
  CATGTCTGTGCCTGCTCTGGTGACTCATCTCAGCACAGTGACCCCTGAGCGCCTCACTGTTTTAGAATCC
  CATGACATGCTTCGTAAATTCATCATATTTTTAAGAGACCAAGATCGATGCCGTGATGTATGTGAAAGTT
  TAGAAGGATGCCATACGCTTTGTCTAATGGGCAACCTCCTACACTTGGGCTCCCTCAGCCCCAGAGTGTT
  AGAGGAGGAGACAGATGGGTTCGTGAGTTTGCTCACCCAGACGCTGTGCTACTGTCGGAAGTATGTGTCT
  CAGAAGAAGTCCAACCTGACCCACTGGCATCCTGTCCTTGGCTGGTTCTCCCAATCTGTGGATTATGGCC
  TTAACGAGTCAATGCACTTGATCACCAAACAGCTGCAGTTCTTGTGGGGGGTGCCTCTGATCCGGATCTT
  CTTCTGTGACATCCTGAGCAAGAAGCTACTGGAGAGCCAGGAGCCAGCCCACGCACAGCCAGCATCCCCT
  CAGAATGTGCTCCCAGTGAAGAGTCTCCTAAAGCGTGCTTTTCAAAAGTCGGCATCAGTCCGGAATATTC
  TCAGGCCTGTCGGGGGTAAACGGGTCGACTCTGCAGAAGTCCAGAAGGTTTGCAACATCTGTGTCCTCTA
  CCAGACCTCGCTGACAACTCTCACACAGATTCGGCTGCAGATACTCACAGGTCTCACTTACCTTGATGAC
  CTGCTTCCCAAACTGTGGGCATTTATCTGTGAGCTCGGGCCCCACGGAGGGTTAAAGCTCTTCTTGGAAT
  GCCTGAACAATGACACTGAAGAGTCCAAGCAACTCTTGGCCATGCTGATGCTGTTCTGTGACTGTTCGCG
  GCACCTCATCACAATCCTTGATGACATTGAAGTTTATGAAGAACAGATTTCATTCAAACTGGAAGAGCTG
  GTCACTATCTCCTCTTTCCTGAATTCTTTTGTGTTTAAGATGATCTGGGATGGAATTGTAGAGAACGCCA
  AGGGTGAGACCTTGGAGCTGTTCCAGTCTGTCCACGGGTGGCTTATGGTGCTGTACGAGCGGGACTGCCG
  GCGGCGCTTCACCCCCGAGGACCACTGGCTGCGAAAGGATCTCAAACCTAGCGTGCTCTTCCAAGAACTC
  GACAGGGACAGAAAACGGGCACAGTTGATCCTGCAGTACATCCCACATGTCATCCCTCACAAAAACAGAG
  TTCTACTGTTTCGAACCATGGTTACCAAGGAGAAGGAGAAACTGGGGCTGGTGGAAACCAGCTCTGCCTC
  CCCGCATGTCACTCACATCACCATCCGCCGGTCCAGGATGCTGGAGGACGGCTACGAGCAGCTTAGGCAG
  CTCTCCCAGCACGCCATGAAGGGGGTCATCCGTGTGAAGTTTGTCAATGACCTCGGGGTGGACGAAGCAG
  GGATTGATCAAGACGGTGTTTTTAAGGAGTTCTTGGAAGAGATCATCAAGAGAGTTTTTGACCCAGCACT
  CAATCTGTTCAAGACAACCAGTGGGGATGAGAGGCTGTACCCCTCACCCACATCCTACATCCATGAGAAT
  TACCTGCAGCTCTTCGAGTTTGTGGGGAAGATGCTGGGGAAGGCTGTGTATGAGGGAATTGTGGTGGACG
  TGCCATTTGCATCCTTCTTCCTGAGCCAACTGCTTGGGCACCACCACAGCGTCTTCTATAGCTCGGTGGA
  TGAACTGCCTTCTCTGGACTCCGAGTTCTATAAAAACCTCACCTCCATCAAGCGCTATGATGGGGACATC
  ACTGACCTGGGCCTGACGCTGTCTTACGACGAGGACGTCATGGGTCAGCTTGTTTGCCATGAACTGATTC
  CTGGAGGGAAGACCATTCCTGTTACAAATGAAAATAAAATTAGCTACATCCATCTGATGGCACATTTTCG
  AATGCACACTCAAATAAAAAACCAAACAGCTGCCCTCATTAGCGGATTCCGTTCCATTATCAAACCCGAG
  TGGATCCGAATGTTCTCAACTCCTGAACTGCAGCGTCTCATCTCTGGCGACAATGCTGAGATTGATCTGG
  AAGATTTAAAGAAGCACACAGTCTACTACGGTGGTTTCCATGGAAGTCACAGAGTCATCATCTGGCTCTG
  GGATATTCTGGCCTCCGACTTCACACCGGATGAGAGAGCTATGTTTCTGAAGTTCGTGACCAGCTGCTCC
  AGACCCCCGCTCCTGGGATTCGCCTACCTCAAGCCTCCATTCTCCATCCGCTGCGTGGAGGTGTCGGACG
  ATCAGGACACCGGGGACACTCTGGGCAGCGTCCTCCGGGGCTTCTTCACCATCCGCAAGCGGGAGCCAGG
  CGGCCGCCTGCCCACCTCCTCCACCTGCTTCAACCTGCTCAAGCTGCCCAACTACAGCAAGAAGAGCGTC
  CTCCGCGAGAAGCTGCGCTACGCCATCAGCATGAACACGGGCTTTGAACTCTCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG219024 representing NM_130466
  Red=Cloning site Green=Tags(s)
  
  MFTLSQTSRAWFIDRARQAREERLVQKERERAAVVIQAHVRSFLCRSRLQRDIRREIDDFFKADDPESTK
  RSALCIFKIARKLLFLFRIKEDNERFEKLCRSILSSMDAENEPKVWYVSLACSKDLTLLWIQQIKNILWY
  CCDFLKQLKPEILQDSRLITLYLTMLVTFTDTSTWKILRGKGESLRPAMNHICANIMGHLNQHGFYSVLQ
  ILLTRGLARPRPCLSKGTLTAAFSLALRPVIAAQFSDNLIRPFLIHIMSVPALVTHLSTVTPERLTVLES
  HDMLRKFIIFLRDQDRCRDVCESLEGCHTLCLMGNLLHLGSLSPRVLEEETDGFVSLLTQTLCYCRKYVS
  QKKSNLTHWHPVLGWFSQSVDYGLNESMHLITKQLQFLWGVPLIRIFFCDILSKKLLESQEPAHAQPASP
  QNVLPVKSLLKRAFQKSASVRNILRPVGGKRVDSAEVQKVCNICVLYQTSLTTLTQIRLQILTGLTYLDD
  LLPKLWAFICELGPHGGLKLFLECLNNDTEESKQLLAMLMLFCDCSRHLITILDDIEVYEEQISFKLEEL
  VTISSFLNSFVFKMIWDGIVENAKGETLELFQSVHGWLMVLYERDCRRRFTPEDHWLRKDLKPSVLFQEL
  DRDRKRAQLILQYIPHVIPHKNRVLLFRTMVTKEKEKLGLVETSSASPHVTHITIRRSRMLEDGYEQLRQ
  LSQHAMKGVIRVKFVNDLGVDEAGIDQDGVFKEFLEEIIKRVFDPALNLFKTTSGDERLYPSPTSYIHEN
  YLQLFEFVGKMLGKAVYEGIVVDVPFASFFLSQLLGHHHSVFYSSVDELPSLDSEFYKNLTSIKRYDGDI
  TDLGLTLSYDEDVMGQLVCHELIPGGKTIPVTNENKISYIHLMAHFRMHTQIKNQTAALISGFRSIIKPE
  WIRMFSTPELQRLISGDNAEIDLEDLKKHTVYYGGFHGSHRVIIWLWDILASDFTPDERAMFLKFVTSCS
  RPPLLGFAYLKPPFSIRCVEVSDDQDTGDTLGSVLRGFFTIRKREPGGRLPTSSTCFNLLKLPNYSKKSV
  LREKLRYAISMNTGFELS
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_130466
• ORF Size: 3204 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_130466.2, NP_569733.2
• RefSeq Size: 5753
• RefSeq ORF: 3207
• Locus ID: 89910
• Protein Families: Druggable Genome
• Protein Pathways: Ubiquitin mediated proteolysis
• Gene Summary: The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: E1 ubiquitin-activating enzymes, E2 ubiquitin-conjugating enzymes, and E3 ubiquitin-protein ligases. This gene encodes a member of the E3 ubiquitin-conjugating enzyme family which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme and transfers the ubiquitin to the targeted substrates. A HECT (homology to E6-AP C-terminus) domain in the C-terminus of the longer isoform of this protein is the catalytic site of ubiquitin transfer and forms a complex with E2 conjugases. Shorter isoforms of this protein which lack the C-terminal HECT domain are therefore unlikely to bind E2 enzymes. Alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2012]