USP4 (NM_199443) Human Tagged ORF Clone

CAT#: RG219178

USP4 (GFP-tagged) - Human ubiquitin specific peptidase 4 (proto-oncogene) (USP4), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: USP4
• Synonyms: UNP; Unph
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG219178 representing NM_199443
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGAAGGTGGAGGCTGCCGTGAGCGACCGGATGCGGAGACTCAGAAGTCCGAGCTTGGACCCTTAA
  TGAGGACCACACTCCAACGCGGGGCGCAGTGGTATCTTATTGACAGCCGGTGGTTCAAGCAGTGGAAGAA
  GTATGTGGGCTTTGACAGCTGGGACATGTACAATGTGGGTGAACATAACCTATTTCCTGGCCCAATAGAC
  AACTCTGGGCTATTTTCAGATCCTGAGAGTCAGACCTTGAAAGAACACTTAATTGATGAATTGGACTATG
  TATTGGTCCCTACCGAGGCGTGGAATAAACTACTAAACTGGTACGGCTGTGTAGAAGGCCAGCAACCCAT
  CGTCAGAAAAGTTGTGGAGCATGGCCTGTTTGTCAAGCACTGCAAAGTCGAGGTGTATTTGCTGGAACTG
  AAGCTCTGTGAGAACAGTGACCCCACCAATGTGCTGAGTTGCCATTTCAGCAAGGCAGACACCATTGCAA
  CCATCGAGAAAGAGATGCGGAAGCTATTCAACATCCCTGCGGAGCGTGAAACACGGCTCTGGAACAAATA
  CATGAGCAACACCTACGAGCAGTTGAGCAAGCTAGACAACACTGTCCAGGATGCTGGGCTATACCAGGGT
  CAGGTGCTAGTAATTGAGCCTCAAAATGAAGATGGCACATGGCCCAGGCAGACCTTGCAGTCAAATGGAT
  CTGGCTTTTCTGCTTCGTATAATTGTCAGGAGCCACCATCCTCTCATATACAACCTGGGCTCTGTGGACT
  TGGAAACCTGGGAAACACCTGCTTCATGAACTCCGCTTTGCAGTGTTTGAGCAACACTGCACCACTGACT
  GACTACTTTCTCAAAGATGAGTATGAAGCCGAAATCAACAGAGACAACCCTCTGGGGATGAAAGGGGAAA
  TTGCAGAAGCCTATGCTGAACTCATTAAGCAGATGTGGTCTGGAAGGGACGCCCATGTGGCACCTCGCAT
  GTTCAAAACTCAAGTAGGACGTTTTGCTCCTCAATTTTCTGGCTACCAGCAACAAGATTCTCAGGAGCTG
  CTGGCCTTTCTTCTAGATGGATTGCATGAAGATCTGAACCGGGTAAAGAAAAAGCCCTACTTGGAGCTGA
  AGGATGCCAATGGGCGGCCAGATGCGGTGGTGGCAAAGGAAGCCTGGGAGAATCACAGGTTGAGGAATGA
  TTCTGTGATTGTGGATACTTTCCATGGCCTCTTCAAATCTACTTTGGTTTGCCCAGAATGTGCTAAGGTT
  TCTGTGACCTTTGACCCATTTTGCTATCTAACGCTGCCACTGCCCTTGAAGAAAGATCGAGTTATGGAGG
  TTTTCCTGGTTCCTGCTGACCCTCACTGCAGACCTACTCAGTACCGTGTGACTGTGCCGCTGATGGGGGC
  TGTGTCCGACCTGTGCGAGGCTCTCTCCAGGCTGTCTGGCATTGCTGCAGAAAATATGGTGGTCGCAGAT
  GTGTATAATCACCGATTCCACAAAATTTTCCAAATGGATGAAGGTTTAAACCACATCATGCCTCGGGATG
  ACATTTTCGTGTACGAGGTCTGCAGCACTTCCGTGGATGGCTCGGAATGTGTCACGCTTCCAGTCTACTT
  CAGGGAGAGGAAGTCCAGGCCATCAAGCACTTCCTCCGCATCAGCGCTATATGGGCAGCCACTATTGCTT
  TCTGTCCCCAAGCACAAGTTAACCCTTGAGTCTTTGTACCAGGCTGTTTGTGATCGTATCAGCCGCTATG
  TGAAACAGCCTTTACCTGATGAGTTTGGCAGCTCACCCTTGGAGCCAGGGGCCTGCAATGGCTCCAGGAA
  CAGCTGTGAAGGAGAAGATGAGGAAGAAATGGAGCATCAGGAAGAAGGCAAAGAGCAGCTTTCAGAAACA
  GAAGGCAGTGGGGAAGATGAGCCAGGAAATGACCCCAGTGAGACCACCCAAAAGAAGATCAAAGGCCAGC
  CCTGCCCAAAAAGGCTTTTTACCTTCAGTCTTGTGAACTCCTATGGAACAGCTGACATAAATTCACTTGC
  AGCTGATGGAAAACTACTTAAACTCAACTCTCGATCTACACTGGCCATGGATTGGGACAGTGAAACTCGG
  AGACTTTACTATGATGAGCAAGAATCTGAGGCCTACGAGAAGCATGTGAGCATGTTGCAGCCTCAGAAGA
  AGAAGAAGACCACAGTGGCCCTGAGAGACTGCATCGAGCTCTTCACCACCATGGAGACCCTTGGGGAGCA
  TGACCCCTGGTACTGTCCCAACTGTAAGAAGCATCAACAGGCCACAAAAAAGTTTGACCTATGGTCCTTG
  CCCAAGATCCTGGTGGTCCACCTCAAACGTTTCTCCTACAACAGATACTGGAGGGATAAGCTCGACACAG
  TCGTAGAATTCCCAATCAGAGGGCTGAACATGTCCGAGTTTGTCTGTAACCTGTCAGCAAGGCCTTATGT
  GTACGACCTCATTGCCGTGTCCAATCATTATGGAGCCATGGGGGTTGGCCACTACACTGCATATGCGAAG
  AACAAACTGAATGGTAAATGGTATTACTTTGATGATAGCAACGTGTCCCTGGCCTCTGAGGATCAGATAG
  TGACTAAAGCAGCTTATGTGCTATTTTACCAACGTCGAGATGATGAATTTTATAAGACACCTTCACTTAG
  CAGTTCTGGTTCCTCTGATGGAGGGACACGACCAAGCAGCTCTCAGCAGGGCTTTGGGGATGATGAGGCT
  TGCAGCATGGACACCAAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG219178 representing NM_199443
  Red=Cloning site Green=Tags(s)
  
  MAEGGGCRERPDAETQKSELGPLMRTTLQRGAQWYLIDSRWFKQWKKYVGFDSWDMYNVGEHNLFPGPID
  NSGLFSDPESQTLKEHLIDELDYVLVPTEAWNKLLNWYGCVEGQQPIVRKVVEHGLFVKHCKVEVYLLEL
  KLCENSDPTNVLSCHFSKADTIATIEKEMRKLFNIPAERETRLWNKYMSNTYEQLSKLDNTVQDAGLYQG
  QVLVIEPQNEDGTWPRQTLQSNGSGFSASYNCQEPPSSHIQPGLCGLGNLGNTCFMNSALQCLSNTAPLT
  DYFLKDEYEAEINRDNPLGMKGEIAEAYAELIKQMWSGRDAHVAPRMFKTQVGRFAPQFSGYQQQDSQEL
  LAFLLDGLHEDLNRVKKKPYLELKDANGRPDAVVAKEAWENHRLRNDSVIVDTFHGLFKSTLVCPECAKV
  SVTFDPFCYLTLPLPLKKDRVMEVFLVPADPHCRPTQYRVTVPLMGAVSDLCEALSRLSGIAAENMVVAD
  VYNHRFHKIFQMDEGLNHIMPRDDIFVYEVCSTSVDGSECVTLPVYFRERKSRPSSTSSASALYGQPLLL
  SVPKHKLTLESLYQAVCDRISRYVKQPLPDEFGSSPLEPGACNGSRNSCEGEDEEEMEHQEEGKEQLSET
  EGSGEDEPGNDPSETTQKKIKGQPCPKRLFTFSLVNSYGTADINSLAADGKLLKLNSRSTLAMDWDSETR
  RLYYDEQESEAYEKHVSMLQPQKKKKTTVALRDCIELFTTMETLGEHDPWYCPNCKKHQQATKKFDLWSL
  PKILVVHLKRFSYNRYWRDKLDTVVEFPIRGLNMSEFVCNLSARPYVYDLIAVSNHYGAMGVGHYTAYAK
  NKLNGKWYYFDDSNVSLASEDQIVTKAAYVLFYQRRDDEFYKTPSLSSSGSSDGGTRPSSSQQGFGDDEA
  CSMDTN
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_199443
• ORF Size: 2748 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_199443.2, NP_955475.1
• RefSeq Size: 3979 bp
• RefSeq ORF: 2751 bp
• Locus ID: 7375
• Cytogenetics: 3p21.31
• Protein Families: Druggable Genome, Protease
• Gene Summary: 'The protein encoded by this gene is a protease that deubiquitinates target proteins such as ADORA2A and TRIM21. The encoded protein shuttles between the nucleus and cytoplasm and is involved in maintaining operational fidelity in the endoplasmic reticulum. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]'