AP3D1 (NM_003938) Human Tagged ORF Clone

CAT#: RG219366

AP3D1 (GFP-tagged) - Human adaptor-related protein complex 3, delta 1 subunit (AP3D1), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: AP3D1
• Synonyms: ADTD; hBLVR; HPS10
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG219366 representing NM_003938
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCTCAAGATGGTGAAGGGCAGCATCGACCGCATGTTCGACAAGAATCTGCAGGACTTGGTCCGCG
  GCATCCGTAACCACAAGGAGGACGAGGCAAAATACATATCTCAGTGCATTGATGAGATCAAGCAGGAGCT
  GAAGCAGGACAACATAGCGGTGAAGGCGAACGCGGTCTGCAAGCTGACGTATTTACAGATGTTGGGATAC
  GACATCAGCTGGGCCGCCTTCAACATCATAGAAGTGATGAGTGCCTCCAAGTTCACCTTCAAGCGAATTG
  GCTACCTCGCTGCTTCCCAGAGCTTTCACGAAGGCACCGACGTCATCATGCTGACCACCAATCAGATCCG
  TAAGGACTTGAGCAGCCCCAGCCAGTACGACACAGGTGTTGCACTGACGGGTCTGTCCTGCTTCGTCACC
  CCAGACCTTGCCAGAGACCTGGCAAATGACATCATGACACTGATGTCACACACCAAGCCCTACATCAGGA
  AGAAGGCTGTGCTGATCATGTACAAGGTGTTCCTGAAGTACCCCGAGTCGCTGCGCCCTGCCTTTCCCCG
  GCTGAAGGAGAAGCTGGAGGACCCCGACCCCGGGGTTCAGTCGGCTGCCGTCAATGTCATCTGCGAGCTG
  GCCAGACGCAACCCTAAGAACTACCTGTCCCTGGCCCCGCTCTTTTTCAAGCTGATGACGTCCTCCACCA
  ACAACTGGGTCCTCATCAAGATCATCAAGCTGTTCGGTGCTCTTACTCCTTTGGAACCGCGGCTGGGCAA
  GAAGCTGATCGAGCCCCTCACCAATCTCATCCACAGCACGTCTGCCATGTCTCTCCTCTATGAATGTGTG
  AACACCGTGATTGCAGTGCTCATCTCGCTGTCCTCCGGCATGCCCAACCACAGCGCCAGCATCCAGCTTT
  GTGTTCAGAAATTAAGGATATTGATCGAGGACTCCGATCAGAACTTGAAGTACCTGGGGCTGCTGGCAAT
  GTCCAAGATCCTGAAGACCCACCCCAAGTCCGTGCAGTCCCACAAGGACCTCATCCTGCAGTGCCTGGAC
  GACAAGGACGAGTCCATCCGGCTGCGGGCCCTGGACCTGCTCTATGGGATGGTGTCCAAGAAGAACCTGA
  TGGAGATCGTGAAGAAGCTGATGACCCACGTAGACAAGGCAGAGGGTACCACCTACCGTGACGAGCTGCT
  CACCAAGATCATTGACATCTGCAGCCAGTCCAACTACCAGTACATCACCAACTTCGAGTGGTACATCAGC
  ATCCTGGTGGAGCTGACCCGGCTGGAGGGCACACGGCACGGCCACCTCATCGCCGCCCAAATGCTGGACG
  TGGCCATCCGCGTGAAGGCCATCCGCAAGTTCGCCGTGTCCCAGATGTCTGCGCTGCTTGACAGTGCACA
  CCTGCTGGCCAGCAGCACCCAGCGGAACGGGATCTGTGAGGTGCTGTACGCTGCCGCCTGGATCTGCGGG
  GAGTTCTCAGAGCATCTGCAGGAACCACACCACACTTTGGAGGCCATGCTGCGGCCCAGAGTCACCACGC
  TGCCAGGCCACATCCAGGCCGTGTATGTGCAGAACGTGGTCAAGCTCTACGCCTCCATCCTGCAGCAGAA
  GGAGCAGGCCGGGGAGGCAGAGGGCGCTCAGGCCGTCACCCAGCTCATGGTGGACCGGCTGCCCCAGTTT
  GTGCAGAGCGCAGACCTGGAGGTGCAGGAGCGGGCGTCCTGCATCCTGCAGCTGGTCAAGCACATCCAGA
  AGCTTCAGGCCAAGGACGTGCCTGTGGCAGAGGAGGTCAGCGCTCTCTTTGCTGGGGAGCTGAACCCAGT
  GGCCCCCAAGGCCCAGAAGAAGGTTCCAGTCCCCGAAGGCCTGGACCTGGACGCCTGGATCAATGAGCCA
  CTCTCGGACAGCGAGTCAGAGGACGAGAGGCCCAGGGCCGTCTTCCACGAGGAGGAGCAGCGGCGTCCCA
  AGCACCGGCCGTCGGAGGCGGACGAGGAAGAGCTGGCTCGGCGCCGAGAGGCCCGGAAGCAGGAGCAGGC
  CAACAACCCCTTCTACATCAAGAGCTCGCCATCGCCACAGAAGCGGTACCAGGACACCCCGGGCGTGGAG
  CACATTCCCGTGGTGCAGATTGACCTCTCCGTCCCCTTGAAGGTTCCAGGGCTGCCTATGTCAGATCAGT
  ATGTGAAGCTGGAGGAGGAGCGGCGGCACCGGCAGAAGCTGGAGAAGGACAAGAGGAGGAAAAAGAGGAA
  GGAGAAGGAGAAGAAGGGCAAGCGCCGCCACAGCTCGCTGCCCACGGAGAGCGACGAGGACATCGCCCCT
  GCCCAGCAGGTGGACATCGTCACAGAGGAGATGCCTGAGAATGCTCTGCCCAGCGACGAGGATGACAAAG
  ACCCCAACGACCCCTACAGGGCTCTGGATATTGACCTGGATAAGCCCTTAGCCGACAGCGAGAAACTGCC
  TATTCAGAAACACAGAAACACCGAGACCTCAAAATCCCCTGAGAAGGACGTTCCCATGGTAGAAAAGAAG
  AGCAAGAAACCCAAGAAGAAAGAGAAAAAACACAAAGAGAAAGAGAGAGACAAGGAGAAGAAGAAGGAGA
  AGGAGAAGAAGAAATCTCCCAAGCCTAAGAAGAAGAAGCACAGGAAGGAGAAGGAGGAGCGGACCAAAGG
  CAAGAAGAAGTCCAAGAAGCAGCCTCCAGGCAGCGAGGAGGCAGCGGGGGAGCCGGTGCAGAATGGCGCG
  CCAGAGGAGGAGCAGCTCCCGCCTGAGTCCAGCTACTCCCTCCTCGCTGAAAATTCCTATGTTAAAATGA
  CCTGTGACATCCGGGGCAGTCTGCAGGAGGACAGCCAGGTCACTGTGGCCATCGTGCTGGAGAACAGGAG
  CAGCAGCATCCTCAAGGGCATGGAGCTCAGCGTGCTGGACTCACTCAATGCCAGGATGGCCCGGCCGCAG
  GGCTCCTCCGTCCACGATGGCGTCCCCGTGCCTTTCCAGCTGCCCCCAGGCGTCTCCAACGAAGCCCAGT
  ATGTGTTCACCATCCAGAGCATCGTCATGGCGCAGAAGCTCAAGGGGACCCTGTCCTTCATTGCCAAGAA
  TGACGAGGGTGCGACCCACGAGAAGCTGGACTTCAGGCTGCACTTCAGCTGCAGCTCCTACTTGATCACC
  ACTCCCTGCTACAGTGACGCCTTTGCTAAGTTGCTGGAGTCTGGGGACTTGAGCATGAGCTCAATCAAAG
  TCGATGGCATTCGGATGTCCTTCCAGAATCTTCTGGCGAAGATCTGTTTTCACCACCATTTTTCCGTTGT
  GGAGCGAGTGGACTCCTGCGCCTCCATGTACAGCCGCTCCATCCAGGGCCACCATGTCTGCCTCCTGGTG
  AAAAAGGGTGAGAACTCTGTCTCAGTCGACGGGAAGTGCAGTGACTCCACGCTACTGAGCAACTTGTTAG
  AAGAGATGAAGGCGACGCTGGCCAAGTGT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG219366 representing NM_003938
  Red=Cloning site Green=Tags(s)
  
  MALKMVKGSIDRMFDKNLQDLVRGIRNHKEDEAKYISQCIDEIKQELKQDNIAVKANAVCKLTYLQMLGY
  DISWAAFNIIEVMSASKFTFKRIGYLAASQSFHEGTDVIMLTTNQIRKDLSSPSQYDTGVALTGLSCFVT
  PDLARDLANDIMTLMSHTKPYIRKKAVLIMYKVFLKYPESLRPAFPRLKEKLEDPDPGVQSAAVNVICEL
  ARRNPKNYLSLAPLFFKLMTSSTNNWVLIKIIKLFGALTPLEPRLGKKLIEPLTNLIHSTSAMSLLYECV
  NTVIAVLISLSSGMPNHSASIQLCVQKLRILIEDSDQNLKYLGLLAMSKILKTHPKSVQSHKDLILQCLD
  DKDESIRLRALDLLYGMVSKKNLMEIVKKLMTHVDKAEGTTYRDELLTKIIDICSQSNYQYITNFEWYIS
  ILVELTRLEGTRHGHLIAAQMLDVAIRVKAIRKFAVSQMSALLDSAHLLASSTQRNGICEVLYAAAWICG
  EFSEHLQEPHHTLEAMLRPRVTTLPGHIQAVYVQNVVKLYASILQQKEQAGEAEGAQAVTQLMVDRLPQF
  VQSADLEVQERASCILQLVKHIQKLQAKDVPVAEEVSALFAGELNPVAPKAQKKVPVPEGLDLDAWINEP
  LSDSESEDERPRAVFHEEEQRRPKHRPSEADEEELARRREARKQEQANNPFYIKSSPSPQKRYQDTPGVE
  HIPVVQIDLSVPLKVPGLPMSDQYVKLEEERRHRQKLEKDKRRKKRKEKEKKGKRRHSSLPTESDEDIAP
  AQQVDIVTEEMPENALPSDEDDKDPNDPYRALDIDLDKPLADSEKLPIQKHRNTETSKSPEKDVPMVEKK
  SKKPKKKEKKHKEKERDKEKKKEKEKKKSPKPKKKKHRKEKEERTKGKKKSKKQPPGSEEAAGEPVQNGA
  PEEEQLPPESSYSLLAENSYVKMTCDIRGSLQEDSQVTVAIVLENRSSSILKGMELSVLDSLNARMARPQ
  GSSVHDGVPVPFQLPPGVSNEAQYVFTIQSIVMAQKLKGTLSFIAKNDEGATHEKLDFRLHFSCSSYLIT
  TPCYSDAFAKLLESGDLSMSSIKVDGIRMSFQNLLAKICFHHHFSVVERVDSCASMYSRSIQGHHVCLLV
  KKGENSVSVDGKCSDSTLLSNLLEEMKATLAKC
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003938
• ORF Size: 3459 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_003938.5, NP_003929.4
• RefSeq Size: 4863
• RefSeq ORF: 3462
• Locus ID: 8943
• Domains: Adaptin_N
• Protein Families: Druggable Genome
• Protein Pathways: Lysosome
• Gene Summary: The protein encoded by this gene is a subunit of the AP3 adaptor-like complex, which is not clathrin-associated, but is associated with the golgi region, as well as more peripheral structures. The AP-3 complex facilitates the budding of vesicles from the golgi membrane, and may be directly involved in trafficking to lysosomes. This subunit is implicated in intracellular biogenesis and trafficking of pigment granules, and possibly platelet dense granules and neurotransmitter vesicles. Defects in this gene are a cause of a new type of Hermansky-Pudlak syndrome. [provided by RefSeq, Feb 2017]