METTL2B (NM_018396) Human Tagged ORF Clone

CAT#: RG219439

METTL2B (GFP-tagged) - Human methyltransferase like 2B (METTL2B)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: METTL2B
• Synonyms: METL; METTL2; METTL2A; PSENIP1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG219439 representing NM_018396
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGGCTCCTACCCTGAAGGTGCACCTGCAATCCTCGCCGATAAGAGGCAGCAGTTCGGAAGCCGGT
  TCCTGAGCGATCCGGCGCGCGTCTTCCACCACAATGCCTGGGACAATGTGGAGTGGTCGGAAGAGCAAGC
  CGCGGCGGCGGAGAGAAAAGTCCAGGAGAACAGTATCCAGCGGGTGTGCCAGGAGAAACAAGTTGATTAT
  GAGATCAATGCCCACAAATACTGGAATGACTTCTACAAAATCCACGAAAATGGGTTTTTCAAGGATAGAC
  ATTGGCTTTTTACCGAATTCCCTGAGCTGGCACCTAGCCAAAATCAAAATCATTTGAAGGATTGGTTCTT
  GGAGAACAAGAGTGAAGTATGTGAATGTAGAAACAATGAGGATGGACCTGGTTTAATAATGGAAGAACAG
  CACAAGTGTTCTTCGAAGAGCCTTGAACATAAAACACAGACACCTCCTGTGGAGGAGAATGTAACTCAGA
  AAATTAGTGACCTGGAAATTTGTGCTGATGAGTTTCCTGGATCCTCAGCCACCTACCGAATACTGGAGGT
  TGGCTGTGGTGTGGGAAACACAGTCTTTCCAATTTTACAAACGAACAATGACCCAGGACTCTTTGTTTAT
  TGCTGTGATTTTTCTTCCACAGCTATAGAACTGGTCCAGACAAATTCAGAATATGATCCTTCTCGGTGTT
  TTGCCTTTGTTCACGACCTGTGTGATGAAGAGAAGAGTTACCCAGTGCCCAAGGGCAGTCTTGATATTAT
  CATTCTCATATTTGTTCTTTCAGCAGTTGTTCCAGACAAGATGCAGAAGGCTATCAACAGGCTGAGCAGG
  CTTCTGAAACCTGGGGGGATGGTACTTCTGCGAGATTACGGCCGCTATGACATGGCTCAGCTTCGGTTTA
  AAAAAGGTCAGTGTCTATCTGGAAATTTCTATGTGAGAGGTGATGGAACCAGAGTTTACTTCTTCACACA
  AGAGGAACTGGACACGCTTTTCACCACTGCTGGACTGGAAAAAGTTCAGAATCTGGTGGACCGCCGACTG
  CAGGTGAACCGAGGGAAGCAACTGACAATGTACCGGGTTTGGATTCAGTGCAAATACTGCAAGCCCCTTC
  TGTCCAGCACCAGC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG219439 representing NM_018396
  Red=Cloning site Green=Tags(s)
  
  MAGSYPEGAPAILADKRQQFGSRFLSDPARVFHHNAWDNVEWSEEQAAAAERKVQENSIQRVCQEKQVDY
  EINAHKYWNDFYKIHENGFFKDRHWLFTEFPELAPSQNQNHLKDWFLENKSEVCECRNNEDGPGLIMEEQ
  HKCSSKSLEHKTQTPPVEENVTQKISDLEICADEFPGSSATYRILEVGCGVGNTVFPILQTNNDPGLFVY
  CCDFSSTAIELVQTNSEYDPSRCFAFVHDLCDEEKSYPVPKGSLDIIILIFVLSAVVPDKMQKAINRLSR
  LLKPGGMVLLRDYGRYDMAQLRFKKGQCLSGNFYVRGDGTRVYFFTQEELDTLFTTAGLEKVQNLVDRRL
  QVNRGKQLTMYRVWIQCKYCKPLLSSTS
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_018396
• ORF Size: 1134 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_018396.2, NP_060866.2
• RefSeq Size: 2192
• RefSeq ORF: 1137
• Locus ID: 55798
• Protein Families: Druggable Genome
• Protein Pathways: Androgen and estrogen metabolism, Histidine metabolism, Selenoamino acid metabolism, Tyrosine metabolism
• Gene Summary: This gene is a member of a family of methyltransferases that share homology with, but are distinct from, the UbiE family of methyltransferases. Alternatively spliced variants which encode different protein isoforms have been described; however, not all variants have been fully characterized. [provided by RefSeq, Jul 2008]