Natriuretic Peptide Receptor C (NPR3) (NM_000908) Human Tagged ORF Clone

CAT#: RG219453

NPR3 (GFP-tagged) - Human natriuretic peptide receptor C/guanylate cyclase C (atrionatriuretic peptide receptor C) (NPR3), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: NPR3
• Synonyms: ANP-C; ANPR-C; ANPRC; C5orf23; GUCY2B; NPR-C; NPRC
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG219453 representing NM_000908
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGTCTCTGCTGGTGCTCACTTTCTCCCCGTGCGTACTACTCGGCTGGGCGTTGCTGGCCGGCGGCA
  CCGGTGGCGGTGGCGTTGGCGGCGGCGGCGGTGGCGCGGGCATAGGCGGCGGACGCCAGGAGAGAGAGGC
  GCTGCCGCCACAGAAGATCGAGGTGCTGGTGTTACTGCCCCAGGATGACTCGTACTTGTTTTCACTCACC
  CGGGTGCGGCCGGCCATCGAGTATGCTCTGCGCAGCGTGGAGGGCAACGGGACTGGGAGGCGGCTTCTGC
  CGCCGGGCACTCGCTTCCAGGTGGCTTACGAGGATTCAGACTGTGGGAACCGTGCGCTCTTCAGCTTGGT
  GGACCGCGTGGCGGCGGCGCGGGGCGCCAAGCCAGACCTTATCCTGGGGCCAGTGTGCGAGTATGCAGCA
  GCGCCAGTGGCCCGGCTTGCATCGCACTGGGACCTGCCCATGCTGTCGGCTGGGGCGCTGGCCGCTGGCT
  TCCAGCACAAGGACTCTGAGTACTCGCACCTCACGCGCGTGGCGCCCGCCTACGCCAAGATGGGCGAGAT
  GATGCTCGCCCTGTTCCGCCACCACCACTGGAGCCGCGCTGCACTGGTCTACAGCGACGACAAGCTGGAG
  CGGAACTGCTACTTCACCCTCGAGGGGGTCCACGAGGTCTTCCAGGAGGAGGGTTTGCACACGTCCATCT
  ACAGTTTCGACGAGACCAAAGACTTGGATCTGGAAGACATCGTGCGCAATATCCAGGCCAGTGAGAGAGT
  GGTGATCATGTGTGCGAGCAGTGACACCATCCGGAGCATCATGCTGGTGGCGCACAGGCATGGCATGACC
  AGTGGAGACTACGCCTTCTTCAACATTGAGCTCTTCAACAGCTCTTCCTATGGAGATGGCTCATGGAAGA
  GAGGAGACAAACACGACTTTGAAGCTAAGCAAGCATACTCGTCCCTCCAGACAGTCACTCTACTGAGGAC
  AGTGAAACCTGAGTTTGAGAAGTTTTCCATGGAGGTGAAAAGTTCAGTTGAGAAACAAGGGCTCAATATG
  GAGGATTACGTTAACATGTTTGTTGAAGGATTCCACGATGCCATCCTCCTCTACGTCTTGGCTCTACATG
  AAGTACTCAGAGCTGGTTACAGCAAAAAGGATGGAGGGAAAATTATACAGCAGACTTGGAACAGAACATT
  TGAAGGTATCGCCGGGCAGGTGTCCATAGATGCCAACGGAGACCGATATGGGGATTTCTCTGTGATTGCC
  ATGACTGATGTGGAGGCGGGCACCCAGGAGGTTATTGGTGATTATTTTGGAAAAGAAGGTCGTTTTGAAA
  TGCGGCCGAATGTCAAATATCCTTGGGGCCCTTTAAAACTGAGAATAGATGAAAACCGAATTGTAGAGCA
  TACAAACAGCTCTCCCTGCAAATCATGTGGCCTAGAAGAATCGGCAGTGACAGGAATTGTCGTGGGGGCT
  TTACTAGGAGCTGGCTTGCTAATGGCCTTCTACTTTTTCAGGAAGAAATACAGAATAACCATTGAGAGGC
  GAACCCAGCAAGAAGAAAGTAACCTTGGAAAACATCGGGAATTACGGGAAGATTCCATCAGATCCCATTT
  TTCAGTAGCT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_000908
• ORF Size: 1620 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000908.2, NP_000899.1
• RefSeq Size: 2651 bp
• RefSeq ORF: 1623 bp
• Locus ID: 4883
• Cytogenetics: 5p13.3
• Domains: ANF_receptor
• Protein Families: Druggable Genome, Transmembrane
• Gene Summary: 'This gene encodes one of three natriuretic peptide receptors. Natriutetic peptides are small peptides which regulate blood volume and pressure, pulmonary hypertension, and cardiac function as well as some metabolic and growth processes. The product of this gene encodes a natriuretic peptide receptor responsible for clearing circulating and extracellular natriuretic peptides through endocytosis of the receptor. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Feb 2011]'