GCOM1 (POLR2M) (NM_015532) Human Tagged ORF Clone

CAT#: RG219621

POLR2M (GFP-tagged) - Human glutamate receptor, ionotropic, N-methyl D-aspartate-like 1A (GRINL1A), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: POLR2M
• Synonyms: GCOM1; Gdown; Gdown1; GRINL1A
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG219621 representing NM_015532
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGCTCGCTGCCCCGCGGCTTCGAGCCCCAAGCTCCCGAGGACTTGGCGCAGCGGAGTTTGGTGGAGC
  TGCGGGAAATGTTGAAGCGCCAGGAGAGACTTTTGCGCAACGAAAAATTCATTTGCAAATTGCCCGACAA
  AGGTAAAAAGATCTTTGACTCTTTTGCCAAACTGAAAGCTGCCATTGCAGAATGTGAAGAAGTTAGAAGA
  AAAAGTGAACTGTTTAACCCTGTTAGTTTAGACTGTAAGCTAAGGCAAAAAGCAATTGCAGAAGTTGATG
  TGGGTACAGATAAGGCCCAGAATTCTGACCCGATACTTGATACTTCATCACTAGTTCCTGGATGTTCCTC
  TGTAGATAACATCAAGTCATCTCAAACCTCACAAAACCAGGGACTTGGACGTCCTACTCTTGAAGGTGAT
  GAAGAGACTTCAGAGGTTGAGTACACAGTGAATAAGGGCCCAGCTTCCAGCAATAGAGACAGGGTACCAC
  CTTCATCTGAAGCTAGTGAGCATCACCCGCGGCATCGTGTTTCAAGTCAAGCGGAAGATACTTCCAGCAG
  CTTTGACAACCTGTTTATTGACAGGTTACAGAGGATCACCATTGCGGACCAAGGTGAACAACAGTCAGAA
  GAAAACGCAAGTACTAAGAACTTGACAGGCCTTTCCAGTGGGACTGAGAAGAAACCTCATTACATGGAAG
  TGCTAGAAATGCGAGCCAAAAACCCAGTGCCCCAGCTGCGTAAATTTAAAACCAATGTGTTACCTTTTCG
  ACAAAATGATTCATCTAGTCATTGCCAGAAGAGTGGGTCTCCTATTTCCTCAGAAGAGCGGCGGCGCAGG
  GATAAGCAGCATCTTGATGACATCACAGCAGCTCGGCTTCTACCACTTCACCATATGCCCACGCAGCTGC
  TCTCCATAGAAGAATCCTTGGCACTTCAGAAACAGCAGAAACAGAATTATGAGGAGATGCAAGCAAAGCT
  CGCAGCGCAAAAATTAGCTGAAAGACTGAATATTAAAATGCGGAGTTATAATCCAGAAGGGGAGTCTTCA
  GGGAGATACCGAGAAGTAAGGGATGAAGATGACGATTGGTCCTCTGATGAATTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG219621 representing NM_015532
  Red=Cloning site Green=Tags(s)
  
  MCSLPRGFEPQAPEDLAQRSLVELREMLKRQERLLRNEKFICKLPDKGKKIFDSFAKLKAAIAECEEVRR
  KSELFNPVSLDCKLRQKAIAEVDVGTDKAQNSDPILDTSSLVPGCSSVDNIKSSQTSQNQGLGRPTLEGD
  EETSEVEYTVNKGPASSNRDRVPPSSEASEHHPRHRVSSQAEDTSSSFDNLFIDRLQRITIADQGEQQSE
  ENASTKNLTGLSSGTEKKPHYMEVLEMRAKNPVPQLRKFKTNVLPFRQNDSSSHCQKSGSPISSEERRRR
  DKQHLDDITAARLLPLHHMPTQLLSIEESLALQKQQKQNYEEMQAKLAAQKLAERLNIKMRSYNPEGESS
  GRYREVRDEDDDWSSDEF
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015532
• ORF Size: 1104 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_015532.3, NP_056347.1
• RefSeq Size: 4139
• RefSeq ORF: 1107
• Locus ID: 81488
• Protein Families: Druggable Genome, Ion Channels: Other
• Gene Summary: This gene encodes a subunit of a specific form of RNA polymerase II termed Pol II(G). The encoded protein may act as a negative regulator of transcriptional activation by the Mediator complex. Alternative splicing results in multiple transcript variants. There is a pseudogene for this gene on chromosome 4. Readthrough transcription between this gene and the neighboring upstream gene MYZAP (myocardial zonula adherens protein) is represented with GeneID 145781. [provided by RefSeq, Oct 2013]