GMPPB (NM_013334) Human Tagged ORF Clone

CAT#: RG219720

GMPPB (GFP-tagged) - Human GDP-mannose pyrophosphorylase B (GMPPB), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: GMPPB
• Synonyms: LGMDR19; MDDGA14; MDDGB14; MDDGC14
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG219720 representing NM_013334
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGGCACTGATCTTAGTGGGGGGCTATGGGACGCGGCTACGGCCGCTGACGCTGAGCACCCCGAAGC
  CACTGGTGGACTTCTGCAATAAGCCCATCTTGCTGCACCAAGTGGAGGCGCTAGCCGCGGCAGGCGTGGA
  CCACGTGATCCTGGCCGTGAGCTACATGTCGCAGGTGCTGGAGAAGGAAATGAAGGCACAGGAGCAGAGG
  CTGGGAATCCGAATCTCCATGTCCCATGAAGAGGAGCCTTTGGGGACAGCTGGGCCCCTGGCGCTGGCCC
  GTGACCTACTCTCTGAGACTGCAGACCCTTTCTTCGTCCTCAACAGTGACGTGATCTGCGATTTCCCCTT
  CCAAGCCATGGTGCAGTTCCACCGGCACCATGGCCAGGAGGGCTCCATCCTGGTGACCAAGGTGGAGGAA
  CCCTCCAAGTACGGTGTGGTGGTGTGTGAGGCTGACACAGGCCGCATTCACCGGTTCGTGGAGAAGCCAC
  AGGTGTTTGTGTCCAATAAGATCAACGCAGGCATGTACATCCTGAGCCCTGCAGTGCTGCGGCGCATCCA
  GCTGCAGCCTACGTCCATTGAGAAGGAGGTCTTCCCCATTATGGCCAAGGAGGGGCAGCTATATGCCATG
  GAGTTACAGGGCTTCTGGATGGACATTGGGCAGCCCAAGGACTTCCTCACTGGCATGTGCCTCTTCCTGC
  AGTCACTGAGGCAGAAGCAGCCTGAGCGGCTGTGCTCAGGCCCTGGCATTGTGGGCAACGTGCTGGTGGA
  CCCAAGTGCCCGCATCGGCCAGAACTGCAGCATTGGCCCCAATGTGAGCCTGGGACCTGGCGTGGTGGTC
  GAAGATGGTGTGTGTATCCGGCGGTGCACGGTGCTGCGGGATGCCCGGATCCGTTCCCATTCCTGGCTTG
  AGTCCTGCATTGTGGGCTGGCGCTGCCGCGTGGGTCAGTGGGTAAGCCTGTGGGCTGGGCTGGGTGGGGA
  GAGGGGCGGGGAGTGTGCCTGCCTCCCTGACAAGGCCTATCCTCTCCTGGAGGTACGCATGGAGAACGTG
  ACAGTGCTGGGTGAGGACGTCATAGTTAATGATGAGCTCTACCTCAACGGAGCCAGCGTGCTGCCCCACA
  AGTCTATTGGCGAGTCAGTGCCAGAGCCTCGTATCATCATG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG219720 representing NM_013334
  Red=Cloning site Green=Tags(s)
  
  MKALILVGGYGTRLRPLTLSTPKPLVDFCNKPILLHQVEALAAAGVDHVILAVSYMSQVLEKEMKAQEQR
  LGIRISMSHEEEPLGTAGPLALARDLLSETADPFFVLNSDVICDFPFQAMVQFHRHHGQEGSILVTKVEE
  PSKYGVVVCEADTGRIHRFVEKPQVFVSNKINAGMYILSPAVLRRIQLQPTSIEKEVFPIMAKEGQLYAM
  ELQGFWMDIGQPKDFLTGMCLFLQSLRQKQPERLCSGPGIVGNVLVDPSARIGQNCSIGPNVSLGPGVVV
  EDGVCIRRCTVLRDARIRSHSWLESCIVGWRCRVGQWVSLWAGLGGERGGECACLPDKAYPLLEVRMENV
  TVLGEDVIVNDELYLNGASVLPHKSIGESVPEPRIIM
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_013334
• ORF Size: 1161 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_013334.2, NP_037466.2
• RefSeq Size: 1664
• RefSeq ORF: 1164
• Locus ID: 29925
• Domains: hexapep, NTP_transferase
• Protein Pathways: Amino sugar and nucleotide sugar metabolism, Fructose and mannose metabolism, Metabolic pathways
• Gene Summary: This gene is thought to encode a GDP-mannose pyrophosphorylase. The encoded protein catalyzes the conversion of mannose-1-phosphate and GTP to GDP-mannose, a reaction involved in the production of N-linked oligosaccharides. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jan 2009]