RPTP mu (PTPRM) (NM_002845) Human Tagged ORF Clone

CAT#: RG219912

PTPRM (GFP-tagged) - Human protein tyrosine phosphatase, receptor type, M (PTPRM), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PTPRM
• Synonyms: hR-PTPu; PTPRL1; R-PTP-MU; RPTPM; RPTPU
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG219912 representing NM_002845
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGGGACTTGGGACTTGCCTGGCGACTTTGGCCGGACTTTTGCTAACTGCGGCGGGCGAGACGTTCT
  CAGGTGGCTGCCTCTTTGATGAGCCGTATAGCACATGTGGATATAGTCAATCTGAAGGTGATGACTTCAA
  TTGGGAGCAAGTGAACACCTTGACTAAACCGACTTCTGATCCATGGATGCCATCAGGTTCTTTCATGCTG
  GTGAATGCCTCTGGGAGACCTGAGGGGCAGAGAGCCCACCTGCTCTTACCCCAACTTAAAGAAAATGACA
  CCCACTGCATCGATTTTCACTATTTTGTGTCCAGCAAGAGTAATTCTCCTCCGGGGTTACTCAATGTCTA
  CGTGAAGGTCAATAACGGGCCACTGGGGAATCCTATCTGGAATATATCTGGAGACCCAACACGTACATGG
  AACAGGGCAGAACTGGCCATTAGTACTTTCTGGCCTAACTTTTATCAGGTGATTTTTGAAGTGATAACTT
  CTGGACATCAAGGCTATCTCGCTATCGATGAGGTGAAGGTGTTAGGACATCCATGTACCAGGACTCCTCA
  CTTCCTGCGGATTCAGAATGTGGAAGTTAATGCTGGCCAGTTTGCTACCTTCCAGTGCAGTGCCATCGGC
  AGGACCGTGGCAGGAGACAGGCTCTGGTTACAGGGCATTGATGTGCGAGATGCTCCTCTGAAGGAAATCA
  AGGTGACCAGCTCCCGACGCTTCATTGCTTCATTTAATGTTGTGAATACCACCAAACGAGATGCTGGAAA
  GTACCGCTGCATGATTCGCACTGAAGGAGGTGTTGGAATATCAAACTATGCAGAGTTGGTAGTTAAAGAA
  CCACCCGTTCCTATTGCCCCACCTCAGCTCGCCTCTGTAGGAGCCACCTACCTGTGGATACAGCTCAACG
  CCAACTCCATCAATGGGGATGGGCCCATTGTGGCCCGAGAGGTGGAGTACTGCACGGCCAGTGGGAGCTG
  GAATGACCGGCAGCCAGTCGATTCCACGAGCTATAAAATTGGACACCTTGACCCAGATACAGAATATGAG
  ATTAGTGTGCTCCTGACCAGGCCAGGGGAGGGTGGCACTGGCTCTCCTGGTCCAGCTCTCAGGACAAGAA
  CAAAGTGTGCTGATCCCATGCGAGGCCCAAGAAAACTAGAAGTAGTGGAGGTCAAATCTCGGCAAATCAC
  TATCCGCTGGGAGCCATTTGGATATAATGTAACTCGTTGCCACAGTTATAATCTCACTGTCCACTACTGT
  TACCAAGTTGGAGGACAAGAACAAGTGCGAGAAGAAGTAAGCTGGGATACAGAAAATTCACACCCTCAAC
  ACACGATCACTAACCTGTCACCATACACCAATGTCAGTGTGAAACTGATCCTCATGAACCCAGAGGGCCG
  GAAGGAAAGCCAAGAACTCATAGTGCAGACAGATGAAGACCTCCCAGGTGCTGTTCCCACTGAATCCATA
  CAAGGAAGTACCTTTGAAGAGAAGATATTTCTTCAGTGGAGAGAACCAACTCAAACATATGGTGTAATCA
  CTTTATATGAGATCACCTACAAAGCAGTCAGTTCCTTTGACCCAGAAATAGATTTATCCAATCAGAGTGG
  AAGAGTTTCAAAGCTGGGAAATGAAACCCATTTTCTGTTTTTTGGACTGTATCCGGGGACCACATACTCC
  TTTACCATCCGAGCTAGCACAGCTAAGGGTTTTGGGCCTCCAGCAACAAACCAGTTCACCACCAAAATAT
  CAGCACCCTCTATGCCAGCTTATGAACTTGAGACACCTTTGAATCAAACTGACAATACCGTGACAGTCAT
  GCTGAAACCTGCCCACAGCAGAGGAGCACCTGTCAGTGTCTATCAAATAGTTGTTGAGGAAGAACGTCCT
  CGAAGAACTAAAAAGACGACAGAAATCTTAAAGTGCTACCCAGTGCCAATTCACTTCCAGAATGCTTCTC
  TGCTGAACTCACAGTACTACTTTGCTGCAGAATTTCCTGCAGACAGCCTCCAAGCTGCGCAGCCTTTTAC
  AATTGGTGATAATAAGACATATAATGGATACTGGAACACTCCCCTTCTCCCCTATAAAAGCTACAGAATT
  TATTTCCAAGCTGCTAGTAGAGCCAATGGGGAAACCAAAATAGACTGTGTCCAAGTGGCCACAAAAGGAG
  CTGCCACTCCGAAACCAGTCCCAGAACCCGAGAAACAGACAGACCATACAGTTAAAATTGCTGGAGTCAT
  CGCGGGCATCTTGCTGTTCGTGATTATATTTCTTGGAGTTGTGTTGGTAATGAAGAAAAGGAAACTGGCC
  AAGAAGCGGAAAGAGACCATGAGCAGCACCCGACAGGAGATGACTGTGATGGTGAACTCAATGGACAAGA
  GCTATGCTGAGCAGGGCACAAACTGCGACGAGGCTTTCTCATTCATGGACACGCACAATCTGAATGGGAG
  ATCTGTGTCTTCACCATCGTCCTTCACAATGAAAACAAATACACTGAGCACATCGGTGCCTAATTCCTAT
  TACCCAGATGAAACCCACACAATGGCCAGCGATACCAGCAGCCTGGTGCAGTCCCATACTTACAAGAAGC
  GAGAGCCGGCCGACGTGCCCTATCAGACTGGGCAGCTCCACCCCGCCATCCGGGTGGCAGACCTCCTTCA
  GCACATCACACAGATGAAGTGTGCGGAGGGCTACGGCTTCAAGGAGGAATACGAGAGCTTCTTTGAAGGG
  CAGTCTGCACCATGGGACTCGGCTAAGAAAGATGAGAACAGAATGAAGAACAGATACGGGAATATCATTG
  CATACGATCATTCCCGAGTGAGGCTGCAGACAATAGAAGGAGACACAAACTCAGACTATATCAATGGCAA
  TTATATCGATGGTTATCATCGACCCAATCATTACATTGCTACCCAAGGGCCAATGCAGGAAACCATCTAT
  GACTTCTGGAGGATGGTGTGGCACGAAAACACTGCAAGTATCATCATGGTGACCAATCTTGTGGAAGTGG
  GAAGGGTCAAATGCTGCAAATACTGGCCAGATGACACAGAGATATATAAAGACATTAAAGTTACCCTAAT
  AGAAACAGAACTACTGGCAGAATATGTGATAAGAACATTTGCTGTTGAAAAGAGAGGTGTGCATGAAATC
  CGAGAGATCAGACAGTTTCACTTCACTGGCTGGCCGGATCATGGGGTCCCCTACCATGCCACCGGCCTGC
  TGGGATTCGTGCGGCAAGTCAAGTCCAAGAGCCCGCCCAGTGCAGGCCCACTGGTGGTGCACTGCAGTGC
  TGGTGCAGGGAGGACTGGCTGTTTCATCGTCATTGATATCATGTTGGACATGGCCGAAAGGGAAGGGGTC
  GTAGACATCTACAACTGCGTCAGGGAGCTGCGGTCACGGAGGGTGAACATGGTGCAAACAGAGGAGCAGT
  ATGTGTTTATCCACGATGCGATCCTGGAAGCCTGTCTTTGTGGGGACACCTCTGTGCCTGCTTCCCAAGT
  TAGGTCTCTGTATTATGACATGAACAAACTGGATCCACAGACAAACTCAAGCCAGATTAAAGAGGAATTC
  CGGACGCTAAACATGGTGACACCAACGCTGCGAGTAGAGGACTGCAGCATCGCACTGTTGCCCCGGAACC
  ATGAGAAAAACCGGTGCATGGACATCCTGCCCCCAGACCGCTGCCTGCCCTTCCTCATCACCATCGATGG
  GGAGAGCAGCAACTACATCAATGCTGCCCTCATGGACAGCTATAAACAGCCTTCAGCTTTTATAGTCACC
  CAGCATCCTTTGCCAAACACAGTGAAAGACTTTTGGAGACTGGTCCTGGATTATCACTGCACATCCGTAG
  TTATGCTAAATGATGTGGATCCTGCCCAGTTGTGTCCACAGTACTGGCCAGAAAACGGAGTACACAGACA
  CGGCCCCATCCAGGTGGAATTTGTCTCTGCTGACCTGGAAGAGGACATCATCAGCAGGATATTCCGCATT
  TACAATGCCGCCAGACCCCAAGATGGATATCGGATGGTGCAGCAATTCCAGTTCCTGGGCTGGCCGATGT
  ACAGGGACACACCAGTGTCTAAGCGCTCCTTCTTGAAGCTCATTCGCCAGGTGGACAAGTGGCAAGAGGA
  ATACAATGGCGGGGAAGGCCGCACCGTTGTGCACTGCTTGAACGGGGGAGGCCGCAGTGGGACGTTCTGC
  GCCATCAGCATCGTATGTGAGATGCTCCGGCACCAGAGAACCGTGGATGTCTTTCACGCTGTGAAGACAC
  TGAGGAACAACAAGCCCAACATGGTCGACCTCCTGGATCAGTACAAGTTCTGCTACGAGGTGGCCCTGGA
  ATACTTGAATTCTGGC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG219912 representing NM_002845
  Red=Cloning site Green=Tags(s)
  
  MRGLGTCLATLAGLLLTAAGETFSGGCLFDEPYSTCGYSQSEGDDFNWEQVNTLTKPTSDPWMPSGSFML
  VNASGRPEGQRAHLLLPQLKENDTHCIDFHYFVSSKSNSPPGLLNVYVKVNNGPLGNPIWNISGDPTRTW
  NRAELAISTFWPNFYQVIFEVITSGHQGYLAIDEVKVLGHPCTRTPHFLRIQNVEVNAGQFATFQCSAIG
  RTVAGDRLWLQGIDVRDAPLKEIKVTSSRRFIASFNVVNTTKRDAGKYRCMIRTEGGVGISNYAELVVKE
  PPVPIAPPQLASVGATYLWIQLNANSINGDGPIVAREVEYCTASGSWNDRQPVDSTSYKIGHLDPDTEYE
  ISVLLTRPGEGGTGSPGPALRTRTKCADPMRGPRKLEVVEVKSRQITIRWEPFGYNVTRCHSYNLTVHYC
  YQVGGQEQVREEVSWDTENSHPQHTITNLSPYTNVSVKLILMNPEGRKESQELIVQTDEDLPGAVPTESI
  QGSTFEEKIFLQWREPTQTYGVITLYEITYKAVSSFDPEIDLSNQSGRVSKLGNETHFLFFGLYPGTTYS
  FTIRASTAKGFGPPATNQFTTKISAPSMPAYELETPLNQTDNTVTVMLKPAHSRGAPVSVYQIVVEEERP
  RRTKKTTEILKCYPVPIHFQNASLLNSQYYFAAEFPADSLQAAQPFTIGDNKTYNGYWNTPLLPYKSYRI
  YFQAASRANGETKIDCVQVATKGAATPKPVPEPEKQTDHTVKIAGVIAGILLFVIIFLGVVLVMKKRKLA
  KKRKETMSSTRQEMTVMVNSMDKSYAEQGTNCDEAFSFMDTHNLNGRSVSSPSSFTMKTNTLSTSVPNSY
  YPDETHTMASDTSSLVQSHTYKKREPADVPYQTGQLHPAIRVADLLQHITQMKCAEGYGFKEEYESFFEG
  QSAPWDSAKKDENRMKNRYGNIIAYDHSRVRLQTIEGDTNSDYINGNYIDGYHRPNHYIATQGPMQETIY
  DFWRMVWHENTASIIMVTNLVEVGRVKCCKYWPDDTEIYKDIKVTLIETELLAEYVIRTFAVEKRGVHEI
  REIRQFHFTGWPDHGVPYHATGLLGFVRQVKSKSPPSAGPLVVHCSAGAGRTGCFIVIDIMLDMAEREGV
  VDIYNCVRELRSRRVNMVQTEEQYVFIHDAILEACLCGDTSVPASQVRSLYYDMNKLDPQTNSSQIKEEF
  RTLNMVTPTLRVEDCSIALLPRNHEKNRCMDILPPDRCLPFLITIDGESSNYINAALMDSYKQPSAFIVT
  QHPLPNTVKDFWRLVLDYHCTSVVMLNDVDPAQLCPQYWPENGVHRHGPIQVEFVSADLEEDIISRIFRI
  YNAARPQDGYRMVQQFQFLGWPMYRDTPVSKRSFLKLIRQVDKWQEEYNGGEGRTVVHCLNGGGRSGTFC
  AISIVCEMLRHQRTVDVFHAVKTLRNNKPNMVDLLDQYKFCYEVALEYLNSG
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002845
• ORF Size: 4356 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002845.2, NP_002836.2
• RefSeq Size: 5065 bp
• RefSeq ORF: 4359 bp
• Locus ID: 5797
• Cytogenetics: 18p11.23
• Domains: Y_phosphatase, MAM, PTPc_motif, IG, FN3
• Protein Families: Druggable Genome, Phosphatase, Transmembrane
• Protein Pathways: Adherens junction, Cell adhesion molecules (CAMs)
• Gene Summary: 'The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP possesses an extracellular region, a single transmembrane region, and two tandem catalytic domains, and thus represents a receptor-type PTP. The extracellular region contains a meprin-A5 antigen-PTP mu (MAM) domain, an Ig-like domain and four fibronectin type III-like repeats. This PTP has been shown to mediate cell-cell aggregation through the interaction with another molecule of this PTP on an adjacent cell. This PTP can interact with scaffolding protein RACK1/GNB2L1, which may be necessary for the downstream signaling in response to cell-cell adhesion. Alternative splicing results in multiple transcripts encoding distinct isoforms. [provided by RefSeq, Jul 2008]'