FER (NM_005246) Human Tagged ORF Clone

CAT#: RG219948

FER (GFP-tagged) - Human fer (fps/fes related) tyrosine kinase (FER)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: FER
• Synonyms: p94-Fer; PPP1R74; TYK3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG219948 representing NM_005246
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGTTTGGGAGTGACCTGAAGAATTCACATGAAGCAGTGTTAAAATTGCAAGACTGGGAATTACGGT
  TACTGGAAACAGTAAAGAAATTTATGGCCCTGAGAATAAAAAGTGATAAAGAATATGCATCTACTTTACA
  GAACCTTTGTAATCAAGTTGATAAGGAAAGTACTGTCCAAATGAATTATGTCAGCAACGTATCCAAGTCT
  TGGCTACTTATGATTCAGCAGACAGAACAACTTAGTAGGATAATGAAGACACATGCAGAGGACTTGAACT
  CTGGACCTTTACACAGGCTCACCATGATGATTAAGGACAAGCAGCAGGTGAAGAAAAGTTACATAGGTGT
  TCATCAGCAGATAGAGGCAGAGATGATCAAGGTTACCAAAACAGAATTGGAGAAGTTAAAATGCAGCTAT
  AGACAATTAATAAAAGAAATGAATTCTGCCAAAGAGAAATATAAAGAAGCTTTAGCTAAAGGGAAGGAAA
  CTGAAAAGGCCAAGGAACGATACGACAAAGCCACAATGAAACTTCATATGTTGCACAATCAGTATGTATT
  GGCGTTGAAAGGGGCACAGCTCCATCAGAATCAGTATTATGATATCACACTTCCCCTGCTTCTGGACTCC
  TTACAAAAGATGCAAGAAGAAATGATAAAAGCACTCAAAGGTATATTTGATGAATACAGCCAGATAACCA
  GTCTTGTCACAGAGGAAATAGTGAATGTCCATAAAGAGATTCAAATGTCGGTTGAACAGATAGATCCTAG
  TACAGAATACAATAATTTCATAGATGTTCACAGAACAACGGCTGCTAAAGAACAAGAAATAGAGTTTGAT
  ACTTCCTTACTGGAAGAAAATGAAAATCTTCAGGCAAATGAGATCATGTGGAATAACTTAACAGCAGAAA
  GTTTGCAAGTAATGTTGAAAACGTTAGCGGAAGAACTTATGCAAACACAGCAGATGCTTTTAAACAAGGA
  GGAGGCTGTTTTGGAGTTAGAGAAGAGAATTGAAGAATCTTCTGAAACTTGTGAGAAGAAGTCTGATATT
  GTGCTTCTGCTAAGCCAAAAACAGGCACTTGAAGAACTGAAACAGTCAGTCCAGCAGCTGAGATGCACTG
  AAGCAAAGTTTTCAGCACAGAAGGAATTACTAGAGCAAAAAGTGCAAGAAAATGATGGGAAAGAGCCACC
  TCCAGTAGTAAATTATGAAGAAGATGCACGATCAGTTACATCTATGGAAAGAAAGGAGAGGCTATCCAAA
  TTTGAATCTATTCGTCATTCAATTGCTGGAATAATTAGGTCTCCAAAATCTGCAGTGGGCTCTTCAGCAC
  TTTCTGATATGATCTCCATCAGTGAGAAGCCTTTGGCAGAACAGGACTGGTACCATGGTGCAATTCCCAG
  AATAGAAGCTCAAGAACTGTTAAAAAAACAAGGAGACTTTTTGGTGCGAGAGAGTCATGGGAAACCTGGT
  GAATATGTCCTTTCTGTATATTCTGATGGACAGAGGAGACATTTTATCATACAATATGTTGATAACATGT
  ATCGATTCGAGGGCACTGGGTTTTCAAACATTCCTCAACTTATAGATCATCACTATACAACAAAACAGGT
  CATCACTAAGAAATCAGGTGTAGTTCTGCTGAATCCTATTCCTAAGGACAAGAAATGGATTCTCAGTCAT
  GAAGATGTCATATTGGGAGAATTACTGGGCAAGGGAAATTTTGGTGAAGTATATAAGGGCACATTAAAGG
  ATAAAACTTCTGTTGCTGTTAAAACATGTAAAGAAGATCTTCCTCAGGAATTGAAAATAAAATTTTTACA
  AGAAGCCAAAATTCTCAAGCAATATGATCATCCCAATATTGTCAAACTTATAGGAGTTTGCACACAAAGA
  CAGCCTGTCTACATCATTATGGAACTGGTTTCAGGAGGTGATTTCCTCACCTTTCTGAGAAGGAAGAAGG
  ATGAACTAAAACTCAAACAGTTAGTGAAATTTTCATTAGACGCTGCTGCTGGTATGTTGTATCTCGAGAG
  TAAAAACTGTATACACAGGGACCTTGCTGCAAGAAACTGCCTGGTAGGTGAAAATAATGTTCTGAAAATC
  AGTGACTTTGGAATGTCTCGTCAAGAGGATGGTGGAGTGTATTCATCTTCTGGCTTAAAGCAGATTCCCA
  TTAAATGGACAGCACCGGAAGCTCTTAATTATGGGAGATACAGTTCAGAGAGTGACGTGTGGAGCTTTGG
  CATCCTTCTCTGGGAGACCTTCAGCTTAGGGGTTTGTCCGTACCCTGGAATGACAAATCAGCAAGCAAGA
  GAGCAAGTAGAAAGAGGATACCGGATGTCAGCTCCCCAGCACTGTCCAGAGGATATTTCCAAAATCATGA
  TGAAGTGTTGGGATTATAAACCTGAAAATCGCCCTAAGTTCAGTGAACTTCAGAAAGAGCTCACTATCAT
  CAAGAGAAAACTCACA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG219948 representing NM_005246
  Red=Cloning site Green=Tags(s)
  
  MGFGSDLKNSHEAVLKLQDWELRLLETVKKFMALRIKSDKEYASTLQNLCNQVDKESTVQMNYVSNVSKS
  WLLMIQQTEQLSRIMKTHAEDLNSGPLHRLTMMIKDKQQVKKSYIGVHQQIEAEMIKVTKTELEKLKCSY
  RQLIKEMNSAKEKYKEALAKGKETEKAKERYDKATMKLHMLHNQYVLALKGAQLHQNQYYDITLPLLLDS
  LQKMQEEMIKALKGIFDEYSQITSLVTEEIVNVHKEIQMSVEQIDPSTEYNNFIDVHRTTAAKEQEIEFD
  TSLLEENENLQANEIMWNNLTAESLQVMLKTLAEELMQTQQMLLNKEEAVLELEKRIEESSETCEKKSDI
  VLLLSQKQALEELKQSVQQLRCTEAKFSAQKELLEQKVQENDGKEPPPVVNYEEDARSVTSMERKERLSK
  FESIRHSIAGIIRSPKSAVGSSALSDMISISEKPLAEQDWYHGAIPRIEAQELLKKQGDFLVRESHGKPG
  EYVLSVYSDGQRRHFIIQYVDNMYRFEGTGFSNIPQLIDHHYTTKQVITKKSGVVLLNPIPKDKKWILSH
  EDVILGELLGKGNFGEVYKGTLKDKTSVAVKTCKEDLPQELKIKFLQEAKILKQYDHPNIVKLIGVCTQR
  QPVYIIMELVSGGDFLTFLRRKKDELKLKQLVKFSLDAAAGMLYLESKNCIHRDLAARNCLVGENNVLKI
  SDFGMSRQEDGGVYSSSGLKQIPIKWTAPEALNYGRYSSESDVWSFGILLWETFSLGVCPYPGMTNQQAR
  EQVERGYRMSAPQHCPEDISKIMMKCWDYKPENRPKFSELQKELTIIKRKLT
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005246
• ORF Size: 2466 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005246.1, NP_005237.1
• RefSeq Size: 2950 bp
• RefSeq ORF: 2469 bp
• Locus ID: 2241
• Cytogenetics: 5q21.3
• Domains: pkinase, SH2, FCH, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Adherens junction
• Gene Summary: 'The protein encoded by this gene is a member of the FPS/FES family of non-transmembrane receptor tyrosine kinases. It regulates cell-cell adhesion and mediates signaling from the cell surface to the cytoskeleton via growth factor receptors. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome X. [provided by RefSeq, Apr 2015]'