DCAMKL2 (DCLK2) (NM_001040261) Human Tagged ORF Clone

CAT#: RG219994

DCLK2 (GFP-tagged) - Human doublecortin-like kinase 2 (DCLK2), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: DCLK2
• Synonyms: CL2; CLICK-II; CLICK2; CLIK2; DCAMKL2; DCDC3; DCDC3B; DCK2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG219994 representing NM_001040261
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCAGCACCAGGAGTATCGAGCTGGAGCACTTTGAGGAACGGGACAAAAGGCCGCGGCCGGGGTCGC
  GGAGAGGGGCCCCCAGCTCCTCCGGGGGCAGCAGCAGCTCGGGCCCCAAGGGGAACGGGCTCATCCCCAG
  TCCGGCGCACAGTGCCCACTGCAGCTTCTACCGCACGCGGACCCTGCAGGCCCTCAGCTCGGAGAAGAAG
  GCCAAGAAGGCGCGCTTCTACCGGAACGGGGACCGCTACTTCAAGGGCCTGGTGTTTGCCATCTCCAGCG
  ACCGCTTCCGGTCCTTCGATGCGCTCCTCATAGAGCTCACCCGCTCCCTGTCGGACAACGTGAACCTGCC
  CCAGGGTGTCCGCACTATCTACACCATCGACGGCAGCCGGAAGGTCACCAGCCTGGACGAGCTGCTGGAA
  GGTGAGAGTTACGTGTGTGCATCCAATGAACCATTTCGTAAAGTCGATTACACCAAAAATATTAATCCAA
  ACTGGTCTGTGAACATCAAGGGTGGGACATCCCGAGCGCTGGCTGCTGCCTCCTCTGTGAAAAGTGAAGT
  AAAAGAAAGTAAAGATTTCATCAAACCCAAGTTAGTGACTGTGATTCGAAGTGGAGTGAAGCCTAGAAAA
  GCCGTGCGGATCCTTCTGAATAAAAAGACTGCTCATTCCTTTGAACAAGTCTTAACAGATATCACCGAAG
  CCATTAAACTAGACTCAGGAGTCGTCAAGAGGCTCTGCACCCTGGATGGAAAGCAGGTTACTTGTCTGCA
  AGACTTTTTTGGTGATGACGATGTTTTTATTGCATGTGGACCAGAAAAATTTCGTTATGCCCAAGATGAC
  TTTGTCCTGGATCATAGTGAATGTCGTGTCCTGAAGTCATCTTATTCTCGATCCTCAGCTGTTAAGTATT
  CTGGATCCAAAAGCCCTGGGCCCTCTCGACGCAGCAAATCACCAGCTTCAGTAAAGCGGGGTGGCCACTA
  CTCCAGTGCCTATTCTACAGCCAAATCCCCAGTTAATGGAACTCCCAGCAGCCAACTTTCTACTCCTAAA
  TCTACGAAATCCTCCAGTTCCTCTCCAACTAGTCCAGGAAGTTTCAGAGGATTAAAGCAGATTTCTGCTC
  ATGGCAGATCTTCTTCCAATGTAAACGGTGGACCTGAGCTTGACCGTTGCATAAGTCCTGAAGGTGTGAA
  TGGAAACAGATGCTCTGAATCATCAACTCTTCTTGAGAAATACAAAATTGGAAAGGTCATTGGTGATGGC
  AATTTTGCAGTAGTCAAAGAGTGTATAGACAGGTCCACTGGAAAGGAGTTTGCCCTAAAGATTATAGACA
  AAGCCAAATGTTGTGGAAAGGAACACCTGATTGAGAATGAAGTGTCAATACTGCGCCGAGTGAAACATCC
  CAATATCATTATGCTGGTCGAGGAGATGGAAACAGCAACTGAGCTCTTTCTGGTGATGGAATTGGTCAAA
  GGTGGAGATCTCTTTGATGCAATTACTTCGTCGACCAAGTACACTGAGAGAGATGGCAGTGCCATGGTGT
  ACAACTTAGCCAATGCCCTCAGGTATCTCCATGGCCTCAGCATCGTGCACAGAGACATCAAACCAGAGAA
  TCTCTTGGTGTGTGAATATCCTGATGGAACCAAGTCTTTGAAACTGGGAGACTTTGGGCTTGCGACTGTG
  GTAGAAGGCCCTTTATACACAGTCTGTGGCACACCCACTTATGTGGCTCCAGAAATCATTGCTGAAACTG
  GCTATGGCCTGAAGGTGGACATTTGGGCAGCTGGTGTGATCACATACATACTTCTCTGTGGATTCCCACC
  ATTCCGAAGTGAGAACAATCTCCAGGAAGATCTCTTCGACCAGATCTTGGCTGGGAAGCTGGAGTTTCCG
  GCCCCCTACTGGGATAACATCACGGACTCTGCCAAGGAATTAATCAGTCAAATGCTTCAGGTAAATGTTG
  AAGCTCGGTGTACCGCGGGACAAATCCTGAGTCACCCCTGGGTGTCAGATGATGCCTCCCAGGAGAATAA
  CATGCAAGCTGAGGTGACAGGTAAACTAAAACAGCACTTTAATAATGCGCTCCCCAAACAGAACAGCACT
  ACCACCGGGGTCTCCGTCATCATGAACACGGCTCTAGATAAGGAGGGGCAGATTTTCTGCAGCAAGCACT
  GTCAAGACAGCGGCAGGCCTGGGATGGAGCCCATCTCTCCAGTTCCTCCCTCAGTGGAGGAGATCCCTGT
  GCCTGGGGAAGCAGTCCCGGCCCCCACCCCTCCGGAATCTCCCACCCCCCACCCTCCTCCCGCTGCCCCG
  GGTGGTGAGCGGGCAGGAACCTGGCGCCGCCACCGAGAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG219994 representing NM_001040261
  Red=Cloning site Green=Tags(s)
  
  MASTRSIELEHFEERDKRPRPGSRRGAPSSSGGSSSSGPKGNGLIPSPAHSAHCSFYRTRTLQALSSEKK
  AKKARFYRNGDRYFKGLVFAISSDRFRSFDALLIELTRSLSDNVNLPQGVRTIYTIDGSRKVTSLDELLE
  GESYVCASNEPFRKVDYTKNINPNWSVNIKGGTSRALAAASSVKSEVKESKDFIKPKLVTVIRSGVKPRK
  AVRILLNKKTAHSFEQVLTDITEAIKLDSGVVKRLCTLDGKQVTCLQDFFGDDDVFIACGPEKFRYAQDD
  FVLDHSECRVLKSSYSRSSAVKYSGSKSPGPSRRSKSPASVKRGGHYSSAYSTAKSPVNGTPSSQLSTPK
  STKSSSSSPTSPGSFRGLKQISAHGRSSSNVNGGPELDRCISPEGVNGNRCSESSTLLEKYKIGKVIGDG
  NFAVVKECIDRSTGKEFALKIIDKAKCCGKEHLIENEVSILRRVKHPNIIMLVEEMETATELFLVMELVK
  GGDLFDAITSSTKYTERDGSAMVYNLANALRYLHGLSIVHRDIKPENLLVCEYPDGTKSLKLGDFGLATV
  VEGPLYTVCGTPTYVAPEIIAETGYGLKVDIWAAGVITYILLCGFPPFRSENNLQEDLFDQILAGKLEFP
  APYWDNITDSAKELISQMLQVNVEARCTAGQILSHPWVSDDASQENNMQAEVTGKLKQHFNNALPKQNST
  TTGVSVIMNTALDKEGQIFCSKHCQDSGRPGMEPISPVPPSVEEIPVPGEAVPAPTPPESPTPHPPPAAP
  GGERAGTWRRHRD
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001040261
• ORF Size: 2349 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001040261.4, NP_001035351.4
• RefSeq Size: 4316
• RefSeq ORF: 2352
• Locus ID: 166614
• Protein Families: Druggable Genome, Protein Kinase
• Gene Summary: This gene encodes a member of the protein kinase superfamily and the doublecortin family. The protein encoded by this gene contains two N-terminal doublecortin domains, which bind microtubules and regulate microtubule polymerization, a C-terminal serine/threonine protein kinase domain, which shows substantial homology to Ca2+/calmodulin-dependent protein kinase, and a serine/proline-rich domain in between the doublecortin and the protein kinase domains, which mediates multiple protein-protein interactions. The microtubule-polymerizing activity of the encoded protein is independent of its protein kinase activity. Mouse studies show that the DCX gene, another family member, and this gene share function in the establishment of hippocampal organization and that their absence results in a severe epileptic phenotype and lethality, as described in human patients with lissencephaly. Multiple alternatively spliced transcript variants have been identified. [provided by RefSeq, Sep 2010]