SCRIBBLE (SCRIB) (NM_182706) Human Tagged ORF Clone

CAT#: RG220211

SCRIB (GFP-tagged) - Human scribbled homolog (Drosophila) (SCRIB), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: SCRIB
• Synonyms: CRIB1; SCRB1; SCRIB1; Vartul
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG220211 representing NM_182706
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTCAAGTGCATCCCGCTGTGGCGCTGCAACCGGCACGTGGAGTCGGTGGACAAGCGGCACTGTTCGC
  TGCAGGCCGTGCCGGAGGAGATCTACCGCTACAGCCGCAGCCTGGAGGAGCTGCTGCTCGACGCCAACCA
  GCTGCGCGAGCTGCCCAAGCCTTTTTTCCGGCTGCTGAACTTGCGCAAGCTGGGCCTGAGCGACAACGAG
  ATCCAGCGGTTGCCTCCCGAGGTGGCCAACTTCATGCAGCTGGTGGAGCTGGACGTGTCCCGGAACGATA
  TCCCTGAGATCCCGGAGAGCATCAAGTTCTGCAAGGCTCTGGAGATCGCGGACTTCAGCGGGAACCCCCT
  CTCCAGGCTCCCTGATGGCTTCACTCAGCTGCGCAGCCTGGCTCACCTGGCCCTGAATGATGTGTCTCTG
  CAGGCACTGCCCGGGGACGTGGGCAACCTCGCCAACCTGGTGACCCTGGAGCTCCGGGAGAACCTGCTCA
  AGTCCCTGCCAGCGTCCCTGTCATTTCTGGTCAAGCTGGAACAGCTGGATCTGGGAGGCAACGATCTGGA
  AGTGCTGCCAGACACTCTGGGGGCTCTGCCCAATCTTCGGGAGCTGTGGCTTGACCGGAACCAGCTGTCA
  GCACTGCCCCCGGAGCTCGGGAACCTGCGGCGCCTGGTGTGCCTGGACGTGTCGGAAAACCGGCTGGAGG
  AGCTGCCTGCTGAGCTCGGCGGGCTGGTGCTGCTCACTGACCTGCTGCTGTCCCAGAACCTGCTGCGGAG
  GCTGCCCGACGGCATCGGTCAGCTGAAGCAGCTATCCATCCTAAAGGTAGACCAGAATCGGCTGTGCGAG
  GTGACTGAGGCCATCGGGGACTGTGAGAACCTCTCTGAGCTGATCCTCACGGAGAACCTGCTGATGGCCC
  TGCCCCGCTCCCTGGGAAAGCTGACTAAGCTGACCAACCTCAACGTGGACCGGAACCACCTCGAGGCGCT
  GCCGCCCGAGATCGGGGGCTGTGTGGCACTCAGCGTCCTCTCCTTGAGGGACAACCGCCTGGCCGTCCTG
  CCACCAGAGCTGGCCCACACGACAGAGCTGCACGTGCTGGACGTGGCGGGGAACCGCCTGCAGAGTCTGC
  CGTTCGCGCTCACCCACCTCAATCTCAAGGCCCTGTGGCTGGCAGAGAACCAGGCGCAGCCCATGCTCCG
  GTTCCAGACGGAGGATGATGCCCGGACCGGCGAGAAGGTGCTCACCTGCTACTTGCTGCCCCAGCAGCCC
  CCACTCAGCCTCGAGGATGCTGGGCAGCAGGGGAGCCTCTCGGAGACCTGGAGCGATGCCCCGCCGAGCC
  GCGTCAGCGTCATCCAGTTCCTGGAGGCCCCCATAGGTGATGAGGACGCTGAGGAAGCTGCAGCTGAGAA
  GCGGGGCCTACAGCGCCGGGCCACACCTCACCCCAGCGAGCTCAAGGTGATGAAGAGGAGCATCGAGGGG
  CGGCGGAGCGAGGCCTGCCCTTGCCAGCCAGACTCTGGGTCGCCCTTGCCTGCAGAGGAGGAGAAGCGGC
  TGAGTGCCGAGTCTGGCCTGAGTGAAGACTCTCGCCCATCTGCCAGCACAGTCTCTGAGGCTGAGCCCGA
  GGGCCCGTCGGCTGAGGCACAGGGTGGGAGCCAGCAGGAAGCCACGACTGCTGGCGGGGAGGAAGACGCC
  GAAGAGGACTACCAGGAGCCCACGGTGCATTTCGCAGAGGACGCACTGCTGCCCGGGGATGACAGGGAGA
  TCGAGGAGGGGCAGCCTGAGGCCCCCTGGACCCTGCCAGGCGGGAGGCAGCGGCTCATCCGCAAGGACAC
  ACCTCACTACAAAAAGCACTTCAAGATCTCCAAGCTGCCCCAGCCCGAGGCCGTTGTGGCTCTGCTGCAG
  GGCATGCAGCCTGATGGGGAGGGCCCTGTGGCTCCCGGGGGCTGGCACAATGGCCCCCACGCACCCTGGG
  CTCCTCGGGCCCAGAAGGAGGAGGAGGAGGAGGAAGAGGGTAGTCCTCAGGAGGAGGAGGAAGAGGAGGA
  GGAGGAAAACAGGGCTGAAGAGGAAGAGGCCAGCACTGAGGAGGAGGACAAGGAGGGGGCCGTGGTTTCT
  GCGCCCTCTGTCAAGGGAGTGTCGTTTGACCAGGCCAATAACCTGCTGATAGAGCCTGCTCGCATTGAGG
  AGGAAGAGCTGACCCTCACCATCCTGCGGCAGACTGGGGGCCTGGGCATCAGCATTGCGGGCGGCAAGGG
  CTCCACACCCTATAAGGGGGACGACGAGGGCATATTCATCTCTCGGGTGTCCGAGGAAGGCCCTGCGGCC
  CGGGCTGGAGTCCGTGTGGGTGACAAGCTCCTGGAGGTGAATGGTGTGGCTCTGCAGGGCGCCGAGCACC
  ACGAGGCCGTGGAGGCGCTCCGGGGGGCCGGCACTGCCGTGCAGATGCGAGTGTGGCGGGAGCGCATGGT
  GGAGCCTGAGAACGCGGTCACCATCACGCCGCTGCGGCCCGAGGATGATTACAGCCCCCGAGAGCGGCGG
  GGAGGGGGGCTGCGCCTGCCCCTGCTCCCGCCTGAGAGCCCCGGGCCCCTCCGTCAGCGCCACGTGGCCT
  GCCTGGCACGCAGCGAGAGGGGGCTGGGCTTCAGCATTGCTGGTGGGAAAGGCTCCACACCCTACAGGGC
  TGGTGATGCGGGCATCTTCGTCTCCCGCATTGCCGAGGGCGGTGCTGCTCACCGCGCGGGCACACTGCAG
  GTTGGCGACCGCGTCCTCTCTATTAATGGAGTGGACGTGACTGAGGCCAGGCATGACCACGCCGTCTCCC
  TGCTGACCGCTGCCTCCCCCACCATCGCCCTGCTGTTGGAGCGGGAGGCTGGGGGCCCTCTTCCTCCCAG
  CCCTCTGCCACATTCCTCACCCCCCACCGCTGCTGTTGCCACCACCAGCATAACCACTGCCACCCCCGGG
  GTGCCTGGGTTGCCGAGCCTGGCCCCCAGCCTGCTGGCTGCCGCGTTGGAAGGGCCATACCCAGTGGAGG
  AGATCCGTCTGCCAAGAGCTGGGGGCCCTCTGGGGCTTAGTATTGTCGGAGGCTCCGACCATTCCAGCCA
  CCCGTTTGGTGTCCAGGAGCCTGGTGTGTTCATCTCCAAGGTGCTCCCGCGGGGCCTGGCCGCTCGCAGC
  GGCCTGCGGGTTGGGGACCGCATCCTGGCAGTGAACGGGCAAGACGTGCGGGATGCCACGCACCAAGAAG
  CAGTCAGTGCCCTGCTCCGGCCCTGCCTGGAGCTGTCGCTGCTGGTGCGGAGGGACCCGGCACCCCCGGG
  CCTACGGGAACTGTGCATCCAGAAGGCACCTGGGGAGAGGCTGGGCATCAGCATCCGCGGGGGTGCCAGG
  GGCCACGCTGGCAACCCCCGCGACCCCACAGACGAGGGCATCTTCATCTCCAAGGTGAGCCCCACGGGGG
  CAGCCGGGCGCGACGGTCGGCTGCGTGTGGGTTTGCGGCTGTTGGAGGTGAACCAGCAGAGCCTGCTGGG
  CCTGACGCACGGCGAGGCGGTGCAGCTGCTCCGCAGTGTGGGCGACACCCTCACCGTGCTGGTCTGTGAC
  GGCTTCGAGGCCAGCACCGACGCAGCCCTGGAGGTGTCCCCAGGTGTCATTGCCAACCCCTTTGCGGCAG
  GCATCGGCCACCGGAACAGCCTGGAGAGCATCTCTTCCATCGACCGGGAGCTGAGCCCTGAGGGCCCAGG
  CAAGGAGAAGGAGCTGCCTGGACAGACCCTGCACTGGGGGCCCGAGGCCACAGAAGCCGCAGGTCGGGGT
  CTGCAGCCCCTGAAGCTGGACTACCGCGCCCTGGCCGCCGTGCCCAGCGCTGGCAGCGTGCAGAGGGTAC
  CGTCTGGAGCAGCTGGAGGGAAGATGGCTGAATCTCCCTGCTCCCCTAGTGGCCAGCAGCCGCCCTCCCC
  GCCTTCTCCGGATGAGCTGCCCGCCAATGTGAAGCAGGCCTACAGGGCCTTCGCGGCCGTGCCCACTTCT
  CACCCGCCTGAGGATGCCCCTGCCCAGCCCCCCACGCCTGGGCCTGCAGCCTCCCCGGAGCAGCTGTCCT
  TCCGGGAGCGGCAGAAGTACTTTGAGCTGGAGGTGCGCGTGCCCCAGGCCGAGGGCCCCCCTAAGCGCGT
  GTCCCTGGTGGGTGCTGACGACCTGCGGAAGATGCAGGAGGAGGAAGCCAGAAAACTACAGCAGAAGAGA
  GCGCAGATGCTGCGGGAGGCGGCAGAGGCTGGGGCCGAAGCGAGGCTCGCCCTGGACGGGGAGACGCTGG
  GCGAGGAGGAACAGGAGGATGAGCAGCCACCCTGGGCCAGCCCGAGCCCCACCTCAAGGCAGAGCCCGGC
  GTCCCCCCCGCCCCTGGGAGGTGGCGCCCCGGTGCGGACGGCCAAAGCTGAACGGCGCCACCAGGAGCGG
  CTGCGCGTGCAGAGTCCGGAGCCACCGGCACCCGAGCGTGCCCTGTCCCCTGCCGAGCTCCGGGCCCTGG
  AGGCCGAGAAGCGTGCGCTGTGGAGGGCAGCCAGGATGAAGTCATTGGAACAGGACGCTCTCCGAGCACA
  GATGGTCCTCAGCAGGTCCCAGGAAGGCCGGGGCACGCGGGGGCCCCTGGAGCGACTGGCCGAGGCCCCT
  TCCCCTGCGCCCACCCCGTCGCCCACCCCTGTGGAAGACCTCGGCCCCCAGACCAGCACCTCCCCGGGAC
  GCCTGCCCTTGTCTGGAAAGAAGTTTGACTACAGGGCCTTTGCGGCCCTGCCTTCTTCCAGACCTGTCTA
  TGACATCCAGTCACCGGACTTTGCTGAGGAGTTGAGGTCCCTGGAACCATCTCCCAGCCCTGGCCCGCAG
  GAGGAGGATGGAGAAGTGGCTCTGGTGCTTCTGGGCAGGCCCTCACCCGGCGCTGTGGGCCCTGAAGATG
  TGGCACTGTGCAGCAGCCGCCGCCCCGTAAGGCCTGGGCGCCGTGGCCTGGGCCCTGTGCCCTCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG220211 representing NM_182706
  Red=Cloning site Green=Tags(s)
  
  MLKCIPLWRCNRHVESVDKRHCSLQAVPEEIYRYSRSLEELLLDANQLRELPKPFFRLLNLRKLGLSDNE
  IQRLPPEVANFMQLVELDVSRNDIPEIPESIKFCKALEIADFSGNPLSRLPDGFTQLRSLAHLALNDVSL
  QALPGDVGNLANLVTLELRENLLKSLPASLSFLVKLEQLDLGGNDLEVLPDTLGALPNLRELWLDRNQLS
  ALPPELGNLRRLVCLDVSENRLEELPAELGGLVLLTDLLLSQNLLRRLPDGIGQLKQLSILKVDQNRLCE
  VTEAIGDCENLSELILTENLLMALPRSLGKLTKLTNLNVDRNHLEALPPEIGGCVALSVLSLRDNRLAVL
  PPELAHTTELHVLDVAGNRLQSLPFALTHLNLKALWLAENQAQPMLRFQTEDDARTGEKVLTCYLLPQQP
  PLSLEDAGQQGSLSETWSDAPPSRVSVIQFLEAPIGDEDAEEAAAEKRGLQRRATPHPSELKVMKRSIEG
  RRSEACPCQPDSGSPLPAEEEKRLSAESGLSEDSRPSASTVSEAEPEGPSAEAQGGSQQEATTAGGEEDA
  EEDYQEPTVHFAEDALLPGDDREIEEGQPEAPWTLPGGRQRLIRKDTPHYKKHFKISKLPQPEAVVALLQ
  GMQPDGEGPVAPGGWHNGPHAPWAPRAQKEEEEEEEGSPQEEEEEEEEENRAEEEEASTEEEDKEGAVVS
  APSVKGVSFDQANNLLIEPARIEEEELTLTILRQTGGLGISIAGGKGSTPYKGDDEGIFISRVSEEGPAA
  RAGVRVGDKLLEVNGVALQGAEHHEAVEALRGAGTAVQMRVWRERMVEPENAVTITPLRPEDDYSPRERR
  GGGLRLPLLPPESPGPLRQRHVACLARSERGLGFSIAGGKGSTPYRAGDAGIFVSRIAEGGAAHRAGTLQ
  VGDRVLSINGVDVTEARHDHAVSLLTAASPTIALLLEREAGGPLPPSPLPHSSPPTAAVATTSITTATPG
  VPGLPSLAPSLLAAALEGPYPVEEIRLPRAGGPLGLSIVGGSDHSSHPFGVQEPGVFISKVLPRGLAARS
  GLRVGDRILAVNGQDVRDATHQEAVSALLRPCLELSLLVRRDPAPPGLRELCIQKAPGERLGISIRGGAR
  GHAGNPRDPTDEGIFISKVSPTGAAGRDGRLRVGLRLLEVNQQSLLGLTHGEAVQLLRSVGDTLTVLVCD
  GFEASTDAALEVSPGVIANPFAAGIGHRNSLESISSIDRELSPEGPGKEKELPGQTLHWGPEATEAAGRG
  LQPLKLDYRALAAVPSAGSVQRVPSGAAGGKMAESPCSPSGQQPPSPPSPDELPANVKQAYRAFAAVPTS
  HPPEDAPAQPPTPGPAASPEQLSFRERQKYFELEVRVPQAEGPPKRVSLVGADDLRKMQEEEARKLQQKR
  AQMLREAAEAGAEARLALDGETLGEEEQEDEQPPWASPSPTSRQSPASPPPLGGGAPVRTAKAERRHQER
  LRVQSPEPPAPERALSPAELRALEAEKRALWRAARMKSLEQDALRAQMVLSRSQEGRGTRGPLERLAEAP
  SPAPTPSPTPVEDLGPQTSTSPGRLPLSGKKFDYRAFAALPSSRPVYDIQSPDFAEELRSLEPSPSPGPQ
  EEDGEVALVLLGRPSPGAVGPEDVALCSSRRPVRPGRRGLGPVPS
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_182706
• ORF Size: 4965 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_182706.2, NP_874365.2
• RefSeq Size: 5221
• RefSeq ORF: 4968
• Locus ID: 23513
• Gene Summary: This gene encodes a protein that was identified as being similar to the Drosophila scribble protein. The mammalian protein is involved in tumor suppression pathways. As a scaffold protein involved in cell polarization processes, this protein binds to many other proteins. The encoded protein binds to papillomavirus E6 protein via its PDZ domain and the C-terminus of E6. Two alternatively spliced transcript variants that encode different protein isoforms have been found for this gene. [provided by RefSeq, Nov 2011]