MAGI1 (NM_004742) Human Tagged ORF Clone

CAT#: RG220279

MAGI1 (GFP-tagged) - Human membrane associated guanylate kinase, WW and PDZ domain containing 1 (MAGI1), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: MAGI1
• Synonyms: AIP-3; AIP3; BAIAP1; BAP-1; BAP1; MAGI-1; MAGI-1b; Magi1d; TNRC19; WWP3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG220279 representing NM_004742
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCAAAGTGATCCAGAAGAAGAACCACTGGACTAGCAGGGTTCACGAATGCACCGTGAAGCGGGGAC
  CCCAGGGCGAGCTGGGGGTGACGGTGCTGGGAGGCGCGGAGCACGGGGAGTTTCCGTACGTCGGAGCGGT
  GGCGGCGGTCGAGGCAGCGGGGCTTCCCGGCGGCGGCGAGGGCCCGAGGCTGGGCGAAGGGGAGCTGCTT
  CTGGAGGTGCAGGGGGTCCGGGTGTCCGGCTTGCCCCGCTATGACGTGCTGGGGGTCATCGACAGCTGCA
  AGGAGGCCGTCACCTTCAAGGCCGTCAGACAAGGAGGAAGGCTGAACAAGGACCTGCGACATTTTCTCAA
  TCAGCGATTCCAGAAGGGGTCTCCTGATCATGAGCTCCAGCAGACCATAAGGGATAACCTTTACCGCCAT
  GCTGTGCCTTGCACAACCCGATCTCCCAGAGAAGGAGAAGTGCCTGGCGTGGACTATAACTTTCTGACTG
  TGAAGGAGTTCTTGGACCTCGAGCAGAGTGGGACTCTTCTGGAAGTCGGCACCTATGAAGGAAACTATTA
  TGGGACACCCAAGCCTCCTAGCCAGCCAGTCAGTGGGAAAGTGATCACGACGGATGCCTTGCACAGCCTT
  CAGTCTGGCTCTAAGCAGTCGACCCCGAAGCGAACCAAGTCCTACAATGATATGCAAAATGCTGGCATAG
  TCCACGCGGAGAATGAGGAGGAGGATGACGTTCCTGAAATGAACAGCAGCTTTACAGCCGATTCTGGTGA
  ACAAGAGGAGCACACTCTCCAAGAAACAGCATTACCACCTGTGAATAGTAGCATCATCGCTGCTCCCATC
  ACGGACCCTTCTCAGAAGTTCCCTCAATACCTACCTCTTTCTGCAGAGGATAATTTAGGTCCTCTACCTG
  AAAACTGGGAGATGGCCTATACTGAAAATGGAGAAGTCTATTTTATAGACCATAACACGAAAACAACATC
  TTGGTTAGACCCTCGGTGCCTAAACAAGCAGCAGAAGCCACTGGAAGAGTGTGAAGATGATGAAGGGGTA
  CACACCGAGGAGCTGGACAGTGAACTAGAACTGCCTGCTGGTTGGGAAAAGATTGAAGACCCTGTCTATG
  GTATCTACTATGTAGACCACATCAACAGGAAGACACAATATGAGAACCCGGTTCTAGAAGCCAAACGGAA
  GAAGCAGCTTGAGCAGCAGCAGCAGCAGCAACAGCAGCAGCAACAGCAGCAGCAGCAGCAGCAGCAGCAG
  CAGACAGAAGAATGGACAGAAGATCACTCAGCCCTTGTGCCTCCTGTTATTCCAAACCACCCTCCAAGCA
  ATCCAGAGCCAGCCAGAGAAGTTCCACTTCAGGGCAAACCCTTTTTTACACGAAACCCTTCTGAGTTGAA
  AGGCAAGTTCATTCACACAAAGCTGCGGAAAAGCAGTCGTGGCTTTGGCTTCACGGTGGTTGGAGGGGAT
  GAACCTGATGAGTTTCTCCAGATCAAGAGCTTGGTCCTAGATGGTCCTGCTGCATTGGATGGCAAGATGG
  AAACAGGGGATGTGATTGTAAGTGTGAATGACACCTGTGTTTTGGGACACACACATGCTCAAGTTGTGAA
  GATCTTCCAGTCCATCCCCATTGGTGCCAGCGTGGACCTTGAACTCTGCCGAGGTTATCCATTGCCTTTT
  GATCCAGATGACCCCAATACAAGTTTAGTGACCTCGGTAGCCATTTTGGATAAAGAACCAATTATTGTGA
  ATGGGCAAGAGACCTATGATTCACCAGCTAGCCACAGTAGTAAAACAGGCAAAGTCAATGGCATGAAGGA
  TGCCAGGCCAAGCAGCCCAGCGGACGTGGCTTCAAATAGTTCTCATGGTTATCCTAATGACACTGTTTCT
  TTGGCTTCCTCCATAGCCACTCAGCCAGAACTCATAACTGTTCATATTGTCAAAGGGCCAATGGGCTTTG
  GTTTTACTATCGCAGACAGTCCTGGTGGGGGTGGCCAAAGAGTGAAACAGATTGTTGACAGCCCAAGGTG
  CCGAGGCCTGAAAGAAGGGGATCTCATAGTGGAAGTTAATAAGAAGAACGTGCAGGCCCTAACTCACAAC
  CAAGTGGTGGATATGCTGGTTGAGTGTCCTAAGGGAAGTGAGGTCACATTGTTGGTGCAACGAGGAGGGC
  TGCCAGTTCCCAAGAAGAGCCCAAAGTCGCAACCACTGGAAAGGAAAGACAGCCAGAATAGTTCTCAGCA
  CAGTGTTTCCAGCCACCGAAGCCTGCACACAGCATCCCCAAGCCACAGCACACAGGTGCTCCCCGAGTTC
  CCACCTGCAGAGGCCCAAGCTCCAGATCAAACTGACAGCTCTGGCCAGAAAAAACCAGATCCTTTTAAAA
  TCTGGGCCCAGTCCAGGAGCATGTATGAAAACCGACCTATGTCACCTTCACCTGCGTCGGGATTGAGCAA
  GGGTGAGAGAGAGAGAGAAATCAATTCCACGAATTTTGGAGAATGTCCGATTCCAGATTACCAGGAACAG
  GACATCTTCCTCTGGAGAAAAGAGACTGGATTTGGATTTAGGATTCTGGGTGGAAATGAACCAGGGGAAC
  CTATTTATATTGGTCACATCGTACCACTGGGTGCTGCTGATACTGACGGCCGCCTGAGGTCTGGAGATGA
  ATTAATCTGTGTGGATGGGACGCCAGTAATTGGAAAATCACACCAGCTTGTGGTCCAGCTTATGCAACAA
  GCTGCCAAGCAAGGCCACGTCAATCTCACGGTGCGGCGTAAAGTGGTTTTTGCGGTGCCCAAAACCGAGA
  ACGAGGTGCCCTCGCCAGCCTCCTCTCATCACAGTAGCAACCAGCCGGCCTCGCTGACAGAAGAGAAGCG
  CACTCCGCAGGGCAGCCAGAACTCGCTGAACACGGTGAGCTCGGGCAGCGGCAGCACCAGCGGCATCGGC
  AGTGGCGGCGGCGGGGGCAGCGGCGTGGTCAGCACCGTGGTGCAGCCCTACGACGTGGAGATCCGGCGCG
  GGGAGAACGAGGGCTTCGGCTTCGTCATCGTGTCCTCGGTGAGCAGGCCCGAAGCAGGCACGACCTTTGC
  AGGCAATGCATGTGTGGCTATGCCTCACAAAATAGGTCGGATTATTGAGGGGAGCCCTGCTGACCGCTGT
  GGCAAGCTGAAAGTAGGAGACCGGATCTTGGCAGTAAATGGATGTTCCATCACCAACAAATCCCATTCAG
  ACATTGTGAACCTAATCAAGGAAGCGGGAAACACAGTTACCCTCCGCATCATTCCTGGGGATGAGTCCTC
  GAATGCCACCTTGCTGACCAATGCAGAGAAGATTGCCACCATCACCACCACACACACCCCTTCTCAGCAA
  GGGACCCAGGAGACAAGGAATACCACCAAACCAAAGCAGGAATCTCAATTTGAGTTCAAAGCACCCCAAG
  CAACACAGGAGCAAGATTTTTACACTGTGGAACTGGAAAGAGGAGCCAAGGGATTTGGCTTTAGCCTTCG
  AGGAGGCCGAGAGTATAACATGGACCTCTATGTTCTGCGCTTAGCAGAGGACGGTCCTGCGGAGAGGTGT
  GGAAAGATGAGGATTGGTGATGAAATTTTAGAGATCAATGGTGAGACCACCAAAAACATGAAGCATTCTC
  GAGCTATAGAACTGATTAAGAATGGTGGCCGCAGAGTTCGTCTGTTTCTGAAGCGGGGAGACGGCTCAGT
  ACCAGAATATGGTGGGTCAAACTATGAAAACATTCCTTCCTTCCCTGGCATGACTCCA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG220279 representing NM_004742
  Red=Cloning site Green=Tags(s)
  
  MSKVIQKKNHWTSRVHECTVKRGPQGELGVTVLGGAEHGEFPYVGAVAAVEAAGLPGGGEGPRLGEGELL
  LEVQGVRVSGLPRYDVLGVIDSCKEAVTFKAVRQGGRLNKDLRHFLNQRFQKGSPDHELQQTIRDNLYRH
  AVPCTTRSPREGEVPGVDYNFLTVKEFLDLEQSGTLLEVGTYEGNYYGTPKPPSQPVSGKVITTDALHSL
  QSGSKQSTPKRTKSYNDMQNAGIVHAENEEEDDVPEMNSSFTADSGEQEEHTLQETALPPVNSSIIAAPI
  TDPSQKFPQYLPLSAEDNLGPLPENWEMAYTENGEVYFIDHNTKTTSWLDPRCLNKQQKPLEECEDDEGV
  HTEELDSELELPAGWEKIEDPVYGIYYVDHINRKTQYENPVLEAKRKKQLEQQQQQQQQQQQQQQQQQQQ
  QTEEWTEDHSALVPPVIPNHPPSNPEPAREVPLQGKPFFTRNPSELKGKFIHTKLRKSSRGFGFTVVGGD
  EPDEFLQIKSLVLDGPAALDGKMETGDVIVSVNDTCVLGHTHAQVVKIFQSIPIGASVDLELCRGYPLPF
  DPDDPNTSLVTSVAILDKEPIIVNGQETYDSPASHSSKTGKVNGMKDARPSSPADVASNSSHGYPNDTVS
  LASSIATQPELITVHIVKGPMGFGFTIADSPGGGGQRVKQIVDSPRCRGLKEGDLIVEVNKKNVQALTHN
  QVVDMLVECPKGSEVTLLVQRGGLPVPKKSPKSQPLERKDSQNSSQHSVSSHRSLHTASPSHSTQVLPEF
  PPAEAQAPDQTDSSGQKKPDPFKIWAQSRSMYENRPMSPSPASGLSKGEREREINSTNFGECPIPDYQEQ
  DIFLWRKETGFGFRILGGNEPGEPIYIGHIVPLGAADTDGRLRSGDELICVDGTPVIGKSHQLVVQLMQQ
  AAKQGHVNLTVRRKVVFAVPKTENEVPSPASSHHSSNQPASLTEEKRTPQGSQNSLNTVSSGSGSTSGIG
  SGGGGGSGVVSTVVQPYDVEIRRGENEGFGFVIVSSVSRPEAGTTFAGNACVAMPHKIGRIIEGSPADRC
  GKLKVGDRILAVNGCSITNKSHSDIVNLIKEAGNTVTLRIIPGDESSNATLLTNAEKIATITTTHTPSQQ
  GTQETRNTTKPKQESQFEFKAPQATQEQDFYTVELERGAKGFGFSLRGGREYNMDLYVLRLAEDGPAERC
  GKMRIGDEILEINGETTKNMKHSRAIELIKNGGRRVRLFLKRGDGSVPEYGGSNYENIPSFPGMTP
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004742
• ORF Size: 3768 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_004742.2, NP_004733.2
• RefSeq Size: 6178
• RefSeq ORF: 3771
• Locus ID: 9223
• Domains: WW, PDZ, Guanylate_kin, GuKc
• Protein Pathways: Tight junction
• Gene Summary: The protein encoded by this gene is a member of the membrane-associated guanylate kinase homologue (MAGUK) family. MAGUK proteins participate in the assembly of multiprotein complexes on the inner surface of the plasma membrane at regions of cell-cell contact. The product of this gene may play a role as scaffolding protein at cell-cell junctions. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]