PDE11A (NM_016953) Human Tagged ORF Clone

CAT#: RG220304

PDE11A (GFP-tagged) - Human phosphodiesterase 11A (PDE11A), transcript variant 4

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PDE11A
• Synonyms: PPNAD2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG220304 representing NM_016953
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGCCTCCCGCCTGGACTTTGGGGAGGTGGAAACTTTCCTGGACAGGCACCCAGAGTTGTTTGAAG
  ATTACTTGATGCGGAAGGGGAAGCAGGAGATGGTTGAAAAGTGGCTGCAGAGGCACAGTCAGGGTCAGGG
  GGCTTTAGGTCCAAGGCCCTCTTTGGCTGGTACCAGCAGCTTGGCTCACAGCACCTGCAGAGGTGGCAGC
  AGCGTTGGTGGTGGCACTGGACCAAATGGCTCTGCCCACAGCCAGCCCCTTCCCGGTGGCGGGGACTGTG
  GTGGGGTTCCCTTGAGTCCCAGCTGGGCCGGTGGCAGCAGGGGCGATGGGAACCTGCAGCGGAGAGCTTC
  TCAGAAAGAGCTAAGGAAGAGTTTTGCCCGCTCCAAGGCCATCCACGTGAACAGGACCTACGATGAACAG
  GTGACCTCCCGGGCTCAGGAACCCCTGAGTAGTGTACGACGGAGGGCACTTCTCCGGAAGGCAAGCTCCC
  TGCCCCCCACCACAGCCCATATTCTCAGTGCGCTGCTGGAATCGAGAGTGAATCTGCCTCAGTATCCCCC
  TACAGCCATCGACTACAAGTGCCATCTGAAAAAGCATAATGAGCGTCAGTTCTTTCTGGAATTGGTCAAA
  GATATCTCCAATGACCTTGACCTCACCAGCCTGAGCTACAAGATTCTCATCTTTGTCTGCCTTATGGTGG
  ATGCTGACCGCTGCTCTCTTTTCCTGGTGGAAGGGGCAGCTGCTGGCAAGAAGACCTTGGTCTCCAAATT
  CTTTGATGTGCATGCAGGAACCCCTCTGCTGCCTTGCAGCAGCACAGAGAACTCAAATGAGGTGCAGGTC
  CCCTGGGGCAAAGGTATCATTGGCTATGTCGGGGAGCATGGAGAAACGGTCAACATTCCTGATGCCTACC
  AGGATCGACGATTCAATGATGAAATCGACAAGCTAACTGGATACAAGACAAAATCATTATTGTGCATGCC
  TATCCGAAGCAGTGATGGTGAGATTATTGGTGTGGCCCAAGCGATAAATAAGATTCCTGAAGGAGCTCCA
  TTTACTGAAGATGATGAAAAAGTTATGCAGATGTATCTTCCATTTTGTGGAATCGCCATATCTAACGCTC
  AGCTCTTTGCTGCCTCAAGGAAAGAATATGAAAGAAGCAGAGCTTTGCTAGAGGTGGTTAATGACCTCTT
  TGAAGAACAGACTGACCTGGAGAAAATTGTCAAGAAAATAATGCATCGGGCCCAAACTCTGCTGAAATGT
  GAACGCTGTTCTGTTTTACTCCTAGAGGACATCGAATCACCAGTGGTGAAATTTACCAAATCCTTTGAAT
  TGATGTCCCCAAAGTGCAGTGCTGATGCTGAGAACAGTTTCAAAGAAAGCATGGAGAAATCATCATACTC
  CGACTGGCTAATAAATAACAGCATTGCTGAGCTGGTTGCTTCAACAGGCCTTCCAGTGAACATCAGTGAT
  GCCTACCAGGATCCGCGCTTTGATGCAGAGGCAGACCAGATATCTGGTTTTCACATAAGATCTGTTCTTT
  GTGTCCCTATTTGGAATAGCAACCACCAAATAATTGGAGTGGCTCAAGTGTTAAACAGACTTGATGGGAA
  ACCTTTTGATGATGCAGATCAACGACTTTTTGAGGCTTTTGTCATCTTTTGTGGACTTGGCATCAACAAC
  ACAATTATGTATGATCAAGTGAAGAAGTCCTGGGCCAAGCAGTCTGTGGCTCTTGATGTGCTATCATACC
  ATGCAACATGTTCAAAAGCTGAAGTTGACAAGTTTAAGGCAGCCAACATCCCTCTGGTGTCAGAACTTGC
  CATCGATGACATTCATTTTGATGACTTTTCTCTCGACGTTGATGCCATGATCACAGCTGCTCTCCGGATG
  TTCATGGAGCTGGGGATGGTACAGAAATTTAAAATTGACTATGAGACACTGTGTAGGTGGCTTTTGACAG
  TGAGGAAAAACTATCGGATGGTTCTATACCACAACTGGAGACATGCCTTCAACGTGTGTCAGCTGATGTT
  CGCGATGTTAACCACTGCTGGGTTTCAAGACATTCTGACCGAGGTGGAAATTTTAGCGGTGATTGTGGGA
  TGCCTGTGTCATGACCTCGACCACAGGGGAACCAACAATGCCTTCCAAGCTAAGAGTGGCTCTGCCCTGG
  CCCAACTCTATGGAACCTCTGCTACCTTGGAGCATCACCATTTCAACCACGCCGTGATGATCCTTCAAAG
  TGAGGGTCACAATATCTTTGCTAACCTGTCCTCCAAGGAATATAGTGACCTTATGCAGCTTTTGAAGCAG
  TCAATATTGGCAACAGACCTCACGCTGTACTTTGAGAGGAGAACTGAATTCTTTGAACTTGTCAGTAAAG
  GAGAATACGATTGGAACATCAAAAACCATCGTGATATATTTCGATCAATGTTAATGACAGCCTGTGACCT
  TGGAGCCGTGACCAAACCGTGGGAGATCTCCAGACAGGTGGCAGAACTTGTAACCAGTGAGTTCTTCGAA
  CAAGGAGATCGGGAGAGATTAGAGCTCAAACTCACTCCTTCAGCAATTTTTGATCGGAACCGGAAGGATG
  AACTGCCTCGGTTGCAACTGGAGTGGATTGATAGCATCTGCATGCCTTTGTATCAGGCACTGGTGAAGGT
  CAACGTGAAACTGAAGCCGATGCTAGATTCAGTAGCTACAAACAGAAGTAAGTGGGAAGAGCTACACCAA
  AAACGACTGCTGGCCTCAACTGCCTCATCCTCCTCCCCTGCCAGTGTTATGGTAGCCAAGGAAGACAGGA
  AC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG220304 representing NM_016953
  Red=Cloning site Green=Tags(s)
  
  MAASRLDFGEVETFLDRHPELFEDYLMRKGKQEMVEKWLQRHSQGQGALGPRPSLAGTSSLAHSTCRGGS
  SVGGGTGPNGSAHSQPLPGGGDCGGVPLSPSWAGGSRGDGNLQRRASQKELRKSFARSKAIHVNRTYDEQ
  VTSRAQEPLSSVRRRALLRKASSLPPTTAHILSALLESRVNLPQYPPTAIDYKCHLKKHNERQFFLELVK
  DISNDLDLTSLSYKILIFVCLMVDADRCSLFLVEGAAAGKKTLVSKFFDVHAGTPLLPCSSTENSNEVQV
  PWGKGIIGYVGEHGETVNIPDAYQDRRFNDEIDKLTGYKTKSLLCMPIRSSDGEIIGVAQAINKIPEGAP
  FTEDDEKVMQMYLPFCGIAISNAQLFAASRKEYERSRALLEVVNDLFEEQTDLEKIVKKIMHRAQTLLKC
  ERCSVLLLEDIESPVVKFTKSFELMSPKCSADAENSFKESMEKSSYSDWLINNSIAELVASTGLPVNISD
  AYQDPRFDAEADQISGFHIRSVLCVPIWNSNHQIIGVAQVLNRLDGKPFDDADQRLFEAFVIFCGLGINN
  TIMYDQVKKSWAKQSVALDVLSYHATCSKAEVDKFKAANIPLVSELAIDDIHFDDFSLDVDAMITAALRM
  FMELGMVQKFKIDYETLCRWLLTVRKNYRMVLYHNWRHAFNVCQLMFAMLTTAGFQDILTEVEILAVIVG
  CLCHDLDHRGTNNAFQAKSGSALAQLYGTSATLEHHHFNHAVMILQSEGHNIFANLSSKEYSDLMQLLKQ
  SILATDLTLYFERRTEFFELVSKGEYDWNIKNHRDIFRSMLMTACDLGAVTKPWEISRQVAELVTSEFFE
  QGDRERLELKLTPSAIFDRNRKDELPRLQLEWIDSICMPLYQALVKVNVKLKPMLDSVATNRSKWEELHQ
  KRLLASTASSSSPASVMVAKEDRN
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016953
• ORF Size: 2802 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_016953.2, NP_058649.2
• RefSeq Size: 4476
• RefSeq ORF: 2802
• Locus ID: 50940
• Domains: PDEase, GAF, HDc
• Protein Families: Druggable Genome
• Protein Pathways: Progesterone-mediated oocyte maturation, Purine metabolism
• Gene Summary: The 3',5'-cyclic nucleotides cAMP and cGMP function as second messengers in a wide variety of signal transduction pathways. 3',5'-cyclic nucleotide phosphodiesterases (PDEs) catalyze the hydrolysis of cAMP and cGMP to the corresponding 5'-monophosphates and provide a mechanism to downregulate cAMP and cGMP signaling. This gene encodes a member of the PDE protein superfamily. Mutations in this gene are a cause of Cushing disease and adrenocortical hyperplasia. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]